Antineoplastic drug NSC631570 modulates functions of hypoxic macrophages

Skivka, Larysa; Fedorchuk, O. G.; Rudyk, Mariia; Позур, В. В.; Khranovska, N.; Grom, Matic; Nowicky, J W

doi:10.3103/s0095452713050095

Cited by 12 publications

(23 citation statements)

References 38 publications

(47 reference statements)

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“…The precipitate (cell suspension) was resuspended in 1 mL of RPMI medium which contained 10% FCS and 40 μg/mL gentamycin. Cell viability was determined using hemocytometer in 0.4% trypan blue exclusion technique (Skivka et al, 2013). Peritoneal macrophages were resuspended in the medium, as outlined above, and diluted to concentration 5 × 10 6 cells/mL.…”

Section: Methodsmentioning

confidence: 99%

“…NO production assay. The NO production by peritoneal macrophages was determined by the accumulation of stable degradation productsnitrites (Skivka et al, 2013). Cells were inoculated (2 × 10 5 cells/mL) in quadruplicates in wells of a polystyrene flat-bottomed 96-well microplate and cultured in RPMI 1640 medium with addition of 10% fetal bovine serum and 1% gentamicin during 18 hours at 37 °C and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…The ROS production by peritoneal macrophages was estimated by NBT assay (Skivka et al, 2013). Then 100 μL of 0.1% nitro-blue tetrazolium chloride (NBT) (Sigma-Aldrich, USA) was added per well and incubated with 0.1% nitro blue tetrazolium (NBT) (Sigma-Aldrich, USA) at 37 °C forg 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…For colorimetric determination of urea, 40 μL of 6% α-ionitrosopropiophenone (α-ISPP) (Sigma-Aldrich, USA) dissolved in 9% ethanol was added to all wells, including the standards (Skivka et al, 2013). Mixtures were incubated at 95 °C for 30 min and then at 4 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

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Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra

Konopelnuk

Kompanets

Svyatetska

et al. 2019

Regul. Mech. Biosyst.

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Progesterone-induced obesity develops in women who use this drug for contraception and the menopause treatment, though its mechanisms remain poorly understood. We studied functional M1 and M2 polarizations of the abdominal cavity macrophages of rats with progesterone induced obesity during 28 days of administration. The effect of melanin from the Antarctic yeast Nadsoniella nigra (Chaetothyriales, Herpotrichiellaceae, Nadsoniella Issatsch, 1914) was investigated. The NO level was determined by the accumulation of nitrites, ROS level was estimated by the NBT-test, arginase activity was assayed by the reaction of L-arginine hydrolysis. The body weights of rats administrated progesterone increased by 27% and continued to increase one month after withdrawal of progesterone (55% higher than control). Melanin prevents the weight gain when administered during one month after progesterone withdrawal. The NO production by peritoneal macrophages of obese animals intensified by 31% indicating their polarization towards pro-inflammatory M1 type. Production of ROS did not change. A 14% increase in arginase activity was observed, indicating the inhibition of M2 (anti-inflammatory) polarization. In the progesterone withdrawal group all these rates significantly decreased, indicating a reduction in the functional activity of peritoneal macrophages’. Melanin decreased the NO and ROS production by 60% and 18% respectively in comparison with the progesterone group and unexpectedly reduced arginase activity. Our data provide evidence of the spread of inflammation in response to progesterone-induced obesity. Peritoneal macrophages are involved in the inflammation in obesity, undergoing polarization towards the pro-inflammatory phenotype. The long-term consequences of such inflammation include the continuation of weight gain and likely the development of systemic inflammation associated with the exhaustion of the functional capacity of peritoneal cavity macrophages. Melanin has an anti-obesity effect and exhibits anti-inflammatory properties preventing progesterone-induced weight gain and macrophage M1 polarization. This requires detailed elucidation and can be valuable in designing countermeasures to prevent obesity outcomes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra

Konopelnuk

Kompanets

Svyatetska

et al. 2019

Regul. Mech. Biosyst.

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“…Brain tissue, which consisted of substantia nigra part, was removed at autopsy. Total protein loads (100 μg of total proteins) extracted from colonic mucosa and brain tissue in a lysis buffer containing protease and phosphatase inhibitors were processed routinely for Western blot as described previously [19]. The primary antibodies against tyrosine hydroxylase (TH) (1 : 500) and β-actin (1 : 500) (Santa Cruz Biotechnology Inc., German) were used to determine for the protein expression levels in the rat colon mucosa and brain tissue with further incubation with anti-rabbit (anti-TH) and anti-mouse (anti-β-actin) secon dary anti-bodies (1 : 2500), conjugated with horseradish peroxidase.…”

Section: Animalsmentioning

confidence: 99%

Role of peripheral dopaminergic system in the pathogenesis of experimental colitis in rats

Prysiazhniuk¹,

Rudyk²,

Chervinska³

et al. 2017

Ukr.Biochem.J.

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dopamine (dA) is produced and released by immune cells. recent data pointed to dA as a key mediator between the nervous and immune systems.In the present study we tested the hypothesis that peripheral dopaminergic system plays a negative role in ulcerative colitis pathogenesis via the effect on activity of peripheral blood phagocytes. The study was conducted on male Wistar rats (170-200 g). The peripheral dopaminergic system was destroyed by 2,3,

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Introduction of antineoplastic drug NSC631570 in an inpatient and outpatient setting: Comparative evaluation of biological effects

Rudyk

Fedorchuk

Susak

et al. 2016

Asian Journal of Pharmaceutical Sciences

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Antineoplastic drug NSC631570 modulates functions of hypoxic macrophages

Cited by 12 publications

References 38 publications

Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra

Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra

Role of peripheral dopaminergic system in the pathogenesis of experimental colitis in rats

Introduction of antineoplastic drug NSC631570 in an inpatient and outpatient setting: Comparative evaluation of biological effects

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