Antimycobacterial and cytotoxic activity of selected medicinal plant extracts

Nguta, Joseph Mwanzia; Appiah–Opong, Regina; Nyarko, Alexander Kwadwo; Yeboah-Manu, Dorothy; Addo, Phyllis; Otchere, Isaac Darko; Kissi-Twum, Abena

doi:10.1016/j.jep.2016.02.010

Cited by 64 publications

(47 citation statements)

References 19 publications

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“…Previous studies that have investigated crude extracts derived from natural product have reported that they exhibit cytotoxic effects against pathogens (24,25). In the current study, PEE treatment on RANKL-stimulated BMMs suppressed TRAP + MNC formation and TRAP activities in a dose-and time-dependent manner.…”

Section: Discussionsupporting

confidence: 52%

Peucedanum japonicum Thunb. ethanol extract suppresses RANKL-mediated osteoclastogenesis

Kim

Erkhembaatar

Lee

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. The constituents of Peucedanum japonicum Thunb. (PJ) exhibit biological and pharmacological activities, including anti-obesity, anti-oxidant and anti-allergic activities. The aim of the present study was to examine in vitro effects of PJ in RANKL-induced signaling pathways, which determine osteoclast differentiation. PJ ethanol extract (PEE) exhibited anti-osteoporotic activity by disrupting the phospholipase C (PLC)-Ca 2+ -c-Fos/cAMP response element-binding protein (CREB)-nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) signaling pathway during osteoclastogenesis. Murine bone marrow-derived macrophages (BMMs) were cultured and used to determine the effects of PJ in the receptor activator of nuclear factor κB ligand (RANKL)-mediated osteoclastogenesis. The effects of PEE in the RANKL-mediated signaling cascade were evaluated using a standard in vitro osteoclastogenesis system. PEE treatment of BMMs significantly reduced the number of RANKL-mediated tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells (P<0.05 for 5 and 10 µg/ml PEE, P<0.01 for 25 and 50 µg/ml PEE), without cytot ox ic ef fe ct s. F u r t her mor e, t he exp r ession of differentiation-related marker genes, including TRAP, Oscar, Cathepsin K, dendrocyte expressed seven ] i increase consequently resulted in the suppression of c-Fos, CREB and NFATc1 activities. These findings highlight the potential use of PJ in treating bone disorders caused by osteoclast overgrowth.

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Section: Discussionsupporting

confidence: 52%

Peucedanum japonicum Thunb. ethanol extract suppresses RANKL-mediated osteoclastogenesis

Kim

Erkhembaatar

Lee

et al. 2017

Experimental and Therapeutic Medicine

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“…Our results were comparable to some of the studies conducted earlier. In a study conducted by Nguta et al, (2016), five selected medicinal plants were assessed for their antimycobacterial activities, one plant extract of Solanum torvum exhibited an MIC below 200 µg/ml, whereas the other extracts' MIC's ranged between 2500 and 10 000 µg/ml on the tested Mycobacterium spp.…”

Section: In Vitro Antimycobacterial Antioxidant Cytotoxic Immunomomentioning

confidence: 99%

“…An IC 50 below 50 µg/ml has been considered to be moderately toxic and samples with a toxicity value higher than 100 µg/ml have been considered to be non-toxic (Vijayarathna and Sasidharan, 2012). In the study mentioned above, the cytotoxicity of the ethanolic extract of Solanum torvum was assessed on human fetal lung fibroblasts and the IC 50 of the extract was found to be 31.25 µg/ml (Nguta et al, 2016). The cytokine levels produced by the treated PBMC's were analysed and evaluated.…”

Section: In Vitro Antimycobacterial Antioxidant Cytotoxic Immunomomentioning

confidence: 99%

In vitro and In vivo antimycobacterial, hepatoprotective and immunomodulatory activity of Euclea natalensis and its mode of action

Lall

Kumar

Meyer

et al. 2016

Journal of Ethnopharmacology

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Ethnopharmacological relevance: The Natal gwarri or Natal ebony (Euclea natalensis A.DC.) is a deciduous tree found widespread throughout southern Africa, especially in Kwazulu-Natal and the southern cost. It has been widely used by indigenous communities such as the Zulus, Tsongas and Vendas for symptoms related to tuberculosis (TB). The decoctions made from the plant parts are administered for chest diseases to treat complications such as chest pains, bronchitis, pleurisy and asthma. TB is prevalent in immune-compromised patients and it is evident that TB-drugs cause hepatotoxicity. The objective of the present study was therefore to evaluate the antimycobacterial activity of the 2 ethanolic extract of E. natalensis against TB and its hepatoprotective and immunomodulatory activities. Materials and methods:The antimycobacterial, antioxidant, hepatoprotective, immunomodulatory activity and cytotoxicity of the ethanolic extract of the shoots of E. natalensis were determined in vitro. The mechanism of action of the antituberculosis activity was determined by investigating the inhibitory effect on mycothiol disulfide reductase enzyme. Furthermore, the acute, sub-acute toxicity (50-2000 mg/kg) and antimycobacterial effect (300 mg/kg) of E. natalensis shoot extract were investigated in Balb/c mice.Hepatoprotective activity of the extract (50-150 mg/kg) was evaluated on isoniazid and rifampicin (50 mg/kg; i.p.) induced hepatic damage in a rat model. Results:The minimum inhibitory concentration of the extract was found to be 125 µg/ml against Mycobacterium tuberculosis. The extracts fifty percent inhibitory concentration (IC 50 ) against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical was found to be 22.55 µg/ml. The plant showed a hepatoprotective effect (50% at 12.5 µg/ml) and the ability to increase Thelper 1 cell cytokines; Interleukin 12, Interleukin 2 and Interferon α by up to 12 fold and the ability to decrease the T-helper 2 cell cytokine Interleukin 10 4 fold when compared to baseline cytokine production. No cellular toxicity was observed in primary peripheral blood mononuclear cells (PBMC's) and two secondary cell lines; U937 monocytes and Chang liver cells (a derivative of the HepG2 cell line). During mechanistic studies, the extract showed a 50% inhibition of mycothiol reductase activity at 38.62 µg/ml. During the acute and subacute studies, E. natalensis exhibited no toxic effect and the fifty percent lethal dose (LD 50 ) was established to be above 2000 mg/kg. The extract was able to reduce the mycobacterial load (1.5-fold reduction) in infected mice. Isoniazid and rifampicin caused significant hepatic damage in rats, and the extract was able to reduce the toxicity by 15% and 40% at 50 and 150 mg/kg respectively. Conclusion:The present study supports the traditional usage of the plant against tuberculosis symptoms. The study showed the ability of E. natalensis shoot extract to inhibit mycobacterial growth, stimulate an appropriate immune response and have a hepatic protective effect. Due to the ...

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“…by using the methanolic extracts of leaves on the model organism female BALB/c mice against M. tuberculosis H 37 Rv at a dose of 200 mg/kg orally administered for 14 days but found no inhibition . However, the high cytotoxicity of hydro‐ethanolic leaf extracts of A. vera is observed against MRC‐5 fibroblast cell line at a dose of 3.20 mg/ml which is a signal to the fact that the investigated crude extract may not be safe for use as a remedy against tuberculosis . According to ethnomedicinal data, other plants being used for the cure of tuberculosis in Himalaya region include Artemisia afra Jacq.…”

Section: Discussionmentioning

confidence: 99%

Review on antibacterial activity of Himalayan medicinal plants traditionally used to treat pneumonia and tuberculosis

Adnan

Ali

Sheikh

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives The main objective of this review was to collect scattered literature on ethnomedicinal plants used to treat pneumonia and tuberculosis in the Himalayan region and their in‐vitro validation against bacterial pathogens. Key findings Current review contains information on ethnomedicines of total 137 plants from Himalaya region. Out of these, 59 plants have been studied in vitro against bacteria while seven plants extracts have been checked for their toxicological effects. The most commonly used plant families for pneumonia and tuberculosis therapy in the study region were Asteraceae, Bignoniaceae and Fabaceae (seven plants in each); of these, Curcuma longa L., Punica granatum L. and Justicia adhatoda L. carried the most inhibiting potential against Staphylococcus aureus and Streptococcus pneumoniae while that of Acalypha indica L. against Mycobacterium tuberculosis. Different compounds such as ascorbic acid, curcumin, vasicine, piperine, quercetin, myricetin and gallic acid being reportedly isolated from these plants possess antibacterial potential. Summary Himalayan region has variety of ethnomedicinal plants used against pneumonia and tuberculosis; however, studies on in‐vivo activity, toxicology and mechanism of action are very limited. Hence, detailed investigation on these aspects needs to be carried out for the development of novel antibacterial drugs from the studied plant species.

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Antimycobacterial and cytotoxic activity of selected medicinal plant extracts

Cited by 64 publications

References 19 publications

Peucedanum japonicum Thunb. ethanol extract suppresses RANKL-mediated osteoclastogenesis

Peucedanum japonicum Thunb. ethanol extract suppresses RANKL-mediated osteoclastogenesis

In vitro and In vivo antimycobacterial, hepatoprotective and immunomodulatory activity of Euclea natalensis and its mode of action

Review on antibacterial activity of Himalayan medicinal plants traditionally used to treat pneumonia and tuberculosis

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