Antimycobacterial activity of two natural alkaloids, vasicine acetate and 2-acetyl benzylamine, isolated from Indian shrub Adhatoda vasica Ness. leaves

Abstract

In folk medicine, Adhatoda vasica Ness. (Acanthaceae) is used to treat asthma and cough. The leaves of A. vasica were powdered and extracted with hexane, ethyl acetate and methanol. The hexane extract showed 97 percent reduction in colony-forming units (CFU) at 100 microg/ml. The hexane extract was subjected to column chromatography. Two natural compounds, vasicine acetate and 2-acetyl benzylamine, were isolated from it. They were bioassayed against Mycobacterium tuberculosis. The two compounds showed strong antimycobacterial activity. Vasicine acetate and 2-acetyl benzylamine isolated from hexane extract of A. vasica leaves, significantly inhibited M. tuberculosis and one multi-drug-resistant (MDR) strain and one sensitive strain at 200 and 50 microg/ml, respectively. Our study demonstrated that both the compounds, vasicine acetate and 2-acetyl benzylamine, could be evaluated further for developing a drug to control M. tuberculosis.

Antimicrobial, antioxidant, cytotoxic and molecular docking properties of N-benzyl-2,2,2-trifluoroacetamide

“…In our earlier communication, we have isolated 2-acetyl benzylamine from Adhatoda vasica leaves and investigated its antimycobacterial activity (Ignacimuthu and Shanmugam 2010). This resulted in the formation of N-benzyl-2,2,2-trifluoroacetamide as expected (Table 1; Fig.…”

Documentation and quantitative analysis of the local knowledge on medicinal plants among traditional Siddha healers in Virudhunagar district of Tamil Nadu, India

“…An open trial (Govindan et al, 1999(Govindan et al, , 2004 indicated that the Solanum trilobatum and Solanum virginianum had significantly lowered the parameters of asthma in mild-moderate asthmatic subjects. The effect of vasicine as bronchodilator (Atal, 1980), vasicine acetate and 2-acetylbenzyl amine as antimycobacterial (Ignacimuthu and Shanmugam, 2010) and an arabinogalactan as antitussive (Chattopadhyay et al, 2011) were reported previously. Zingiber officinale (Ahui et al, 2008), Ocimum tenuiflorum (Sridevi et al, 2009), Calotropis gigantea (Vadnere et al, 2010), Plectranthus amboinicus were reported previously for anti-inflammatory and mast cell stabilizing effect.…”

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“…Vasicine acetate was more effective than 2-acetyl benzylamine at all concentrations. [43] In support of above studies, Barry et al [44] and Gupta and Chopra [45] have also reported the antitubercular potential of AV.…”