Antimycobacterial activity and molecular docking of methanolic extracts and compounds of marine fungi from Saldanha and False Bays, South Africa

Tapfuma, Kudzanai Ian; Nyambo, Kudakwashe; Adu-Amankwaah, Francis; Baatjies, Lucinda; Smith, Liezel; Allie, Nasiema; Keyster, Marshall; Loxton, André G.; Ngxande, Mkhuseli; Malgas-Enus, Rehana; Mavumengwana, Vuyo

doi:10.1016/j.heliyon.2022.e12406

Cited by 7 publications

(11 citation statements)

References 60 publications

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“…C. rhizophaga has been reported to cause black spots in water chestnuts (Yang et al, 2019), necrosis of leaf and stem tissue in Dendrocalamus giganteus (Zazzerini et al, 2010) and wilt in chickpea (Abang et al, 2009). However, C. rogersoniana is primarily associated with the synthesis of bioactive metabolites for treating human diseases (Chanthasena et al, 2021; De Oliveira et al, 2022; Tapfuma et al, 2022; Yin et al, 2017). It is also used in the biological control of fungal diseases, nematode infestations (Pecundo et al, 2021; Zhao et al, 2021) and pathogenic entomopathogenic fungi associated with some agricultural pests (Sahin & Yanar, 2021; Sonmez et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Identification of Clonostachys rogersoniana as a causal agent of sisal leaf blight disease in South China

Li,

Wang,

et al. 2023

Journal of Phytopathology

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A novel strain of leaf blight disease affecting sisal leaves was discovered at the Hongshan farm of Yulin City, Guangxi Province, in 2022. Ten fungal isolates were identified in the infected sisal leaves and classified into six categories (22GX3‐1 to 6). Pathogenicity testing revealed that only 22GX3‐2 and two morphologically similar isolates induced symptoms similar to those detected in the field. Based on morphological analysis and nucleotide sequencing of the internal transcribed spacer (ITS) of the rRNA gene, translation elongation factor 1 alpha (TEF1) and tubulin beta chain 2 (TUB2) genes, the fungal isolate 22GX3‐2 was identified as Clonostachys rogersoniana. To the best of our knowledge, this is the first study reporting sisal leaf blight caused by Clonostachys rogersoniana.

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Section: Resultsmentioning

confidence: 99%

Identification of Clonostachys rogersoniana as a causal agent of sisal leaf blight disease in South China

Li,

Wang,

et al. 2023

Journal of Phytopathology

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“…Penicillium brevicompactum was isolated from an unidentified brown mushroom in a forest that was dominated by Celtis africana according to the method described in the work of Tapfuma [17]. The isolate was revived by carefully slicing a small portion of the fungi that was previously grown on a potato dextrose agar (PDA) plate using a sterile scalpel under a laminar flow hood, where the cutting was transferred to a fresh PDA plate.…”

Section: Isolation and Identification Of The Fungal Organismmentioning

confidence: 99%

“…Fresh cultures of P. brevicompactum were prepared by cutting a portion of the grown culture in PDA and placing it on fresh PDA plates and incubated at 30 • C for 14 days. The ribosomal DNA of the organism was extracted and the internal transcriber spacer 1 and 2 (ITS 1 and 2) was amplified using the primer pairs reported in the study by Tapfuma et al [17] including the phylogenetic analysis approach. However, the outlier organism was Clonostachys rogersoniana MGK33.…”

Section: Isolation and Identification Of The Fungal Organismmentioning

confidence: 99%

“…After the respective incubation periods, the mixture was centrifuged at 4000 rpm for 15 min to remove the cell debris. Thereafter, equal volumes of the supernatant and ethyl acetate were then mixed in a separating funnel and allowed to stand for approximately 30 min [17]. The solvent phase was then concentrated in a rotary evaporator set at 60 • C and the resulting mixture was dried for a period of 7 days in an oven (22-25 • C) to remove any remaining solvent.…”

Section: Gaa Bioconversionmentioning

confidence: 99%

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Untargeted LC-QTOF-MS Analysis of Metabolites Produced by Penicillium brevicompactum during the Bioconversion of Ganoderic Acid A

Naidoo,

Mavumengwana,

Tapfuma

et al. 2023

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The repurposing of expired drugs through bioconversion remains one of the most crucial research milestones, as this practice reduces drug contamination while producing compounds of significance. The present study investigated the bioconversion of ganoderic acid A (GAA) using Penicillium brevicompactum over a period of 3, 6 and 9 days. The GAA intensity reduced from 22,099 cps on day 0 to 11,040, 4700 and 18,126 cps on day 3, 6 and 9, respectively, thus demonstrating the degradation of GAA over time. The produced metabolites that were recovered using ethyl acetate as a solvent were determined using LC-QTOF-MS. P. brevicompactum produced a variety of compounds in the absence of GAA, while in its presence, it was observed that P. brevicompactum was able to convert GAA and produced ganomastenol A/B/D, vitamin E succinate, and aminopregnane on day 3, while on day 6, armillaripin and ganolucidic acid A were produced. After 9 days of operation, vitamin E succinate, ganolucidic acid A and lucilactaene were produced. The present study is the first report on the ability of P. brevicompactum to bioconvert GAA. The identified metabolites have been established to possess bioactivity against various ailments, thus contributing to the discovery of new drugs.

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“…One way to overcome this challenge is to genetically modify M.tb in order to investigate, identify, and validate new TB drug targets whose potential inhibitors can speed up and boost the current regimen ( 11 – 14 ). A second approach is to extract and investigate natural compounds from the environment (sea, soil, and plants) that could sometimes be even more potent than the current drugs ( 15 , 16 ). A third approach is to investigate host enzymes that can be modulated during TB treatment to booster therapy ( 17 – 19 ).…”

Section: Introductionmentioning

confidence: 99%

Spermine enhances the activity of anti-tuberculosis drugs

Sao Emani,

Reiling

2024

Microbiol Spectr

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The discovery of molecules that could shorten the treatment period of tuberculosis (TB) holds the promise of improving the current regimen. Polyamines are organic polycationic alkylamines found in millimolar concentrations in all living cells. Spermine (Spm) is a polyamine that was shown (more than 70 years ago) to be toxic to Mycobacterium tuberculosis (M.tb). In this study, we used various biochemical assays to assess the mechanism of action of Spm. Furthermore, using various drug susceptibility testing methods, we investigated the ability of Spm to enhance the activity of first-line TB drugs, isoniazid (INH) and rifampicin (RIF), as well as second-line TB drugs, para-aminosalicylic acid (PAS) and bedaquiline (BDQ). Our findings revealed that at toxic concentrations, Spm induced the formation of reactive oxygen species (ROS), a reaction that was influenced by the pH and the iron concentration of the media. Furthermore, the generation of ROS by Spm was associated with increased ferric iron and NADP levels. Moreover, the ROS produced by Spm synergized with the ROS generated by the lipophilic peroxide cumene hydroperoxide (CuOOH), as well as with the ROS generated by RIF and PAS, while enhancing their antimycobacterial activity. Additionally, we found that Spm enhanced the combined activity of INH and RIF against M.tb. Lastly, Spm was also able to enhance the activity of BDQ due to another mechanism of action. This study provides data and findings that would fuel future studies on the suitability of Spm as a potential adjunct TB therapy. The enhanced antimycobacterial activity of TB drugs in combination with Spm could offer promising new avenues for improving TB treatment outcomes. IMPORTANCE This is the first study that attempted to demonstrate the mechanisms of reactive oxygen species (ROS) generation by spermine (Spm) in Mycobacterium tuberculosis (M.tb). Furthermore, this is the first study to demonstrate that it is able to enhance the activity of currently available and World Health Organization (WHO)-approved tuberculosis (TB) drugs. Spermine can easily be obtained since it is already found in our diet. Moreover, as opposed to conventional antibiotics, it is less toxic to humans since it is found in millimolar concentrations in the body. Finally, with the difficulty of curing TB with conventional antibiotics, this study suggests that less toxic molecules, such as Spm, could in a long-term perspective be incorporated in a TB regimen to boost the treatment.

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Antimycobacterial activity and molecular docking of methanolic extracts and compounds of marine fungi from Saldanha and False Bays, South Africa

Cited by 7 publications

References 60 publications

Identification of Clonostachys rogersoniana as a causal agent of sisal leaf blight disease in South China

Identification of Clonostachys rogersoniana as a causal agent of sisal leaf blight disease in South China

Untargeted LC-QTOF-MS Analysis of Metabolites Produced by Penicillium brevicompactum during the Bioconversion of Ganoderic Acid A

Spermine enhances the activity of anti-tuberculosis drugs

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