Antimutagenicity of Stevia pilosa and Stevia eupatoria evaluated with the Ames test

Cariño-Cortés, Raquel; Hernández-Ceruelos, Alejandra; Torres-Valencia, J. Martı́n; González-Ávila, Marisela; Arriaga-Alba, Myriam; Madrigal-Bujaidar, Eduardo

doi:10.1016/j.tiv.2006.12.001

Cited by 28 publications

(13 citation statements)

References 36 publications

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“…However, their chemical composition has been reported, which helped us to explain their effects. Stevia pilosa and Stevia eupatoria extracts have compounds such as luteolin, quercetin, β-sitosterol, stigmasterol [11], seco-triterpenes [17], and longipinenes [18]. Luteolin has shown cellular arrest and apoptosis induction in many types of cancer cell lines, such as prostate cancer (PC-3), liver cancer (SMMC7721), colon cancer (COLO205), and cervical cancer (HeLa) [19].…”

Section: Discussionmentioning

confidence: 99%

“…Of all the Stevia species that exist, approximately 70 species are native to Mexico, and their anticancer activity has rarely been studied. Stevia pilosa and Stevia eupatoria are used for the treatment of stomach pains as a hypoglycemic agent, and as a diuretic, analgesic, anti-inflammatory, antihypertensive, and mutagenesis protector in cells exposed to alkylating agents [11]. These effects are attributed to their compounds such as flavonoids, sterols, sesquiterpenes, and longipinenes, which possess important biological activities [12].…”

Section: Introductionmentioning

confidence: 99%

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Stevia Eupatoria and Stevia Pilosa Extracts Inhibit the Proliferation and Migration of Prostate Cancer Cells

et al. 2020

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Background and Objectives: Prostate cancer is the second most harmful disease in men worldwide and the number of cases is increasing. Therefore, new natural agents with anticancer potential should be examined and the response of existing therapeutic drugs must be enhanced. Stevia pilosa and Stevia eupatoria are two species that have been widely used in traditional medicine, but their effectiveness on cancer cells and their interaction with antineoplastic drugs have not been studied. The aim of this study was to evaluate the anticancer activity of Stevia pilosa methanolic root extract (SPME) and Stevia eupatoria methanolic root extract (SEME) and their effect, combined with enzalutamide, on prostate cancer cells. Materials and Methods: The study was conducted on a human fibroblast cell line, and on androgen-dependent (LNCaP) and androgen-independent (PC-3) prostate cancer cell lines. The cell viability was evaluated using a Trypan Blue exclusion test for 48 h, and the migration by a wound-healing assay for 24, 48, and 72 h. Results: The results indicate that SPME and SEME were not cytotoxic at concentrations less than 1000 μg/mL in the human fibroblasts. SPME and SEME significantly reduced the viability and migration of prostate cancer cells in all concentrations evaluated. The antiproliferative effect of the Stevia extracts was higher in cancer cells than in normal cells. The enzalutamide decreased the cell viability in all concentrations tested (10–50 µM). The combination of the Stevia extracts and enzalutamide produced a greater effect on the inhibition of the proliferation and migration of cancer cells than the Stevia extracts alone, but not of the enzalutamide alone. Conclusion: The results indicate that SPME and SEME have an inhibitory effect on the viability and migration of prostate cancer cells and do not interfere with the enzalutamide anticancer effect. The data suggest that Stevia extracts may be a potential source of molecules for cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stevia Eupatoria and Stevia Pilosa Extracts Inhibit the Proliferation and Migration of Prostate Cancer Cells

et al. 2020

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“…Many studies of natural plants, such as Stevia pilosa , Stevia eupatoria , and Castela texana , have exhibited antimutagenic and antigenotoxic properties [1, 2]. Among these plants, it has been reported that antioxidants, a mixture of components that can reduce reactive oxygen species (ROS), induce mutagens or improve alkylated DNA damage [3, 4].…”

Section: Introductionmentioning

confidence: 99%

Antimutagenicity of Methanolic Extracts from Anemopsis californica in Relation to Their Antioxidant Activity

Del‐Toro‐Sánchez

Bautista-Bautista

Blasco-Cabal

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Anemopsis californica has been used empirically to treat infectious diseases. However, there are no antimutagenic evaluation reports on this plant. The present study evaluated the antioxidant activity in relation to the mutagenic and antimutagenic activity properties of leaf (LME) and stem (SME) methanolic extracts of A. californica collected in the central Mexican state of Querétaro. Antioxidant properties and total phenols of extracts were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Folin-Ciocalteu methods, respectively. Mutagenicity was evaluated using the Ames test employing Salmonella enterica serovar Typhimurium strains (TA98, TA100, and TA102), with and without an aroclor 1254 (S9 mixture). Antimutagenesis was performed against mutations induced on the Ames test with MNNG, 2AA, or 4NQO. SME presented the highest antioxidant capacity and total phenolic content. None of the extracts exhibited mutagenicity in the Ames test. The extracts produced a significant reduction in 2AA-induced mutations in S. typhimurium TA98. In both extracts, mutagenesis induced by 4NQO or methyl-N′-nitro-N-nitrosoguanidine (MNNG) was reduced only if the exposure of strains was <10 μg/Petri dish. A. californca antioxidant properties and its capacity to reduce point mutations render it suitable to enhance medical cancer treatments. The significant effect against antimutagenic 2AA suggests that their consumption would provide protection against carcinogenic polycyclic aromatic compounds.

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“…In the Ames test, Stevia eupatoria (Spreng.) Wild (Asteraceae) extracts significantly reduce mutations induced by 2-amino-anthracene (2AA) added to Salmonella typhimurium TA98, which is sensitive to the frameshift mutagens (Cariño-Cortés et al, 2007). Because of its ability to reduce CYP450 expression, affinin may reduce mutations induced by polycyclic aromatic hydrocarbons, which require metabolic activation for mutagenic transformation (Rodeiro et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Antimutagenic properties of affinin isolated fromHeliopsis longipesextract

Arriaga-Alba

Rios

Déciga-Campos

2013

Pharmaceutical Biology

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Context: Heliopsis longipes (A. Gray) Blake (Asteraceae), commonly known in Mexico as ''chilcuage'' or ''chilcuan'', is widely used as an analgesic and anesthetic agent. Affinin, the major metabolite of this plant, and the ethanol extract of the plant have shown antinociceptive properties in mice. H. longipes plant produces a complex mixture of antioxidant chlorophylls and polyamines as well as a number of possible antimutagens. Objective: The current study evaluated the potential utilization of the natural product affinin isolated from H. longipes ethanol extract as an antimutagenic and possibly anticarcinogenic agent. Materials and methods: The Ames assay was used to assess the mutagenic properties of affinin (12.5, 25 and 50 mg/plate) that was added to several mutagens with or without S9 metabolic activation in Salmonella typhimurium (TA98, TA100 and TA102 strains). Results: Heliopsis longipes extract and affinin were not toxic as a reduction in the number of His þ revertant bacteria colonies. Affinin (25 and 50 mg/plate) significantly reduced the frameshift mutations that were generated by 2-aminoanthracene (2AA) (40%) and reduced the oxidative DNA damage generated by norfloxacin (NOR) (37-50%). Affinin possessed antioxidant properties that were able to reduce 2AA-and NOR-induced mutations in S. typhimurium TA98 and TA102, respectively. Discussion and conclusion: Affinin, the principal metabolite of H. longipes, is not mutagenic and possesses antimutagenic activity. These plants are currently used to treat some pain symptoms in Mexico; and antimutagen activity determined could be important to treat some pain symptoms related to antiradical activity.

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Antimutagenicity of Stevia pilosa and Stevia eupatoria evaluated with the Ames test

Cited by 28 publications

References 36 publications

Stevia Eupatoria and Stevia Pilosa Extracts Inhibit the Proliferation and Migration of Prostate Cancer Cells

Stevia Eupatoria and Stevia Pilosa Extracts Inhibit the Proliferation and Migration of Prostate Cancer Cells

Antimutagenicity of Methanolic Extracts from Anemopsis californica in Relation to Their Antioxidant Activity

Antimutagenic properties of affinin isolated fromHeliopsis longipesextract

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