Antimutagenic potential of harpagoside and Harpagophytum procumbens against 1-nitropyrene

Abstract

Background:1-nitropyrene (1-NPy) is one of the most abundant nitro-polycyclic aromatic hydrocarbons particularly in diesel exhausts. It is a mutagenic and carcinogenic pollutant very widespread in the environment. So the discovery of antimutagenic agents is essential. Harpagophytum procumbens (HP) is traditionally used as anti-inflammatory and analgesic particularly against painful osteoarthritis. Harpagoside (HS), its major iridoid glycoside, is considered as the main active component.Objective:The aim of the present study was to evaluate the antimutagenic activity of HS and HP extracts against mutagenic activity of 1-NPy.Materials and Methods:The antimutagenic activity was investigated using the in vitro cytokinesis-block micronucleus assay in cultured human lymphocytes. Cells were exposed to HS or HP extracts before (pretreatment), during (co-treatment), and after (posttreatment) treatment with 1-NPy.Results:Results showed that HS significantly reduced the mutagenicity of 1-NPy in pretreatment and particularly in co-treatment, whereas all HP extracts significantly reduced the genotoxicity in the three protocols.Conclusion:These results suggested that HS was strongly involved in antimutagenic activity of HP extracts in co-treatment, but other components in HP extracts participated in this activity in pre- and post-treatment.

The Use of Harpagophytum Procumbens (Martynia Fragrans) in Rheumatology: a Look through the Prism of Comorbidity (Literature Review)

“…In particular, Mncwangi et al (2012), in addition to antimalarial and uterotonic properties, also indicate to anticancer ones [32]. Harpagosides of this plant demonstrate antimutagenic effect at environmental damages [31]. A number of researchers have reported on its ability to lower blood pressure, heart rate and increase force of contraction, promote blood thinning [18,32,37].…”

“…It should also be considered that most patients with osteoarthritis are of elderly and senile age often with a predisposition to oncopathology. This plant has antimutagenic properties in environmental damages [31].…”

The Use of Harpagophytum Procumbens (Martynia Fragrans) in Rheumatology: a Look through the Prism of Comorbidity (Literature Review)

“…Показано, що застосування екстракту кореня мартинії запашної дозволяє прискорити регрес больового синдрому та зменшити потребу у застосуванні НПЗП, а згодом і відмовитися від вживання останніх, застосовуючи у подальшому тільки екстракт кореня мартинії в дозах 1000-3000 мг/ добу тривалістю від двох до шести місяців залежно від клінічної ситуації [7,9] [32]. Гарпагозиди цієї рослини виявляють антимутагенну дію при середовищному пошкодженні [31]. Про здатність знижувати артеріальний тиск, частоту серцевих скорочень, збільшувати силу серцевих скорочень та сприяти розрідженню крові повідомляє низка дослідників [18,32,37].…”

“…Слід також мати на увазі, що більшість хворих на ОА -це люди літнього і старечого віку зі схильністю також і до онкопатології. Зазначеній рослині притаманні антимутагенні властивості при середовищному пошкодженні [31].…”

Bioactive Compounds and Extracts from Traditional Herbs and Their Potential Anti-Inflammatory Health Effects

“…Its bioactivity is conferred by iridoids, a family of monoterpenoids. The glycoside fraction (containing mainly harpagoside; Figure 3 ) in devil’s claw has been proven to be antimutagenic against environmental injury [ 23 ].…”