Antimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cells

Menoli, R.; Mantovani, Mário Sérgio; Ribeiro, Lúcia Regina; Speit, Günter; Jordão, Berenice Quinzani

doi:10.1016/s1383-5718(01)00227-3

Cited by 97 publications

(57 citation statements)

References 17 publications

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“…AB SP extract showed the highest efficiency, while those of AB 97/29 (young phase) and AB mix did not exhibit any antigenotoxic activity in pre-treatment. These variable responses observed in the comet assay correspond with those reported by Menoli et al (2001).…”

Section: Discussionsupporting

confidence: 89%

“…A selective tumoricidal effect of soluble proteoglucan extracted from A. blazei Murrill, mediated via natural killer-cell activation and by the induction of apoptosis, was demonstrated by Fujimiya et al (1999). The A. blazei mushroom teas prepared from mixed strains (AB 96/07, AB 96/09 and AB 97/11) studied in vitro and in vivo, by Menoli et al (2001) and Delmanto et al (2001) respectively, showed antimutagenic activity against methyl methanesulfonate (MMS) and cyclophosphamide. On the other hand, a previous study conducted by Guterres et al (2004), which assessed the antimutagenicity effect of aqueous extracts from young and sporulated basidiocarps of the AB 97/29 strain in vitro, could not show a protective effect against MMS.…”

Section: Introductionmentioning

confidence: 99%

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Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

et al. 2005

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Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 ® 6)b-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

et al. 2005

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“…These effects have been identified by our group mainly using aqueous extracts and tests for the induction of macrolesions in DNA, such as chromosomal aberrations (Bellini et al, 2003;Bellini et al, 2006) and micronuclei formation (Menoli et al, 2001;Oliveira et al, 2002). However, the chemoprotective effect in A. blazei aqueous extracts has not always been demonstrated and our group has shown that the antimutagenicity effects of water extracts of A. blazei can vary because while a V79 Chinese hamster lung cell micronucleus test showed an antigenotoxic effect of A. blazei aqueous extracts against the mutagen methanesulfonate (MMS) a comet test using the same cells did not (Guterrez et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Various A. blazei extracts obtained using different solvents have been evaluated and the protective potential of compounds present in these extracts has been the subject of much published research, some by our group (Delmanto et al, 2001;Menoli et al, 2001;Luiz et al, 2003a;Machado et al, 2005;Bellini et al, 2006) and some by other researchers (Sorimachi et al, 2001;Kasai et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…The basidiomycete A. blazei has been extensively consumed as a food and therapeutic agent and aqueous extracts of this fungi have been substantially investigated for their antimutagenic, antigenotoxic, anticarcinogenic and anticlastogenic potentials in various test systems by our group (Delmanto et al, 2001; Menoli et al, 2001;Barbisan et al, 2002;Bellini et al, 2003;Machado et al, 2005;Bellini et al, 2006) and by other researchers (Osaki et al, 1994).…”

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Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays

et al. 2008

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Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). Different methanolic extract fractions (ME) of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN) clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT) assay for gene mutation, both using Chinese hamster ovary cells clone K1 (CHO-K1). The results suggest that all the fractions tested possess cytotoxic and mutagenic potential but no clastogenic effects. Further information is needed on the biochemical components of the A. blazei methanol fractions to identify any substances with cytotoxic and/or mutagenicity potential. These findings indicate that A. blazei methanolic extract should not be used due to their genotoxicity and care should be taken in the use of A. blazei by the general population until further biochemical characterization of this fungi is completed.

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Mutagenicity and Antimutagenicity of Medicinal Plants

Musarrat

Aqil

Ahmad

2006

Modern Phytomedicine

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Plants synthesize an array of structurally and functionally diverse bioactive secondary metabolites. These phytocompounds are subject to wide experimental scrutiny for their pharmacological and therapeutic potential. Substantial work has been reported on the screening of medicinal and edible plants for their mutagenicity and/or antimutagenic properties. Most of these natural products are regarded as potential sources of novel therapeutic agents against mutational disorders in humans. For a successful screening program, it is essential to evaluate critically the biological activity of plant extracts through appropriate assay systems. This chapter presents information related to traditionally used medicinal and edible plants endowed with a variety of phytochemicals conferring mutagenicity and antimutagenicity. 13.1

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Antimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cells

Cited by 97 publications

References 17 publications

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays

Mutagenicity and Antimutagenicity of Medicinal Plants

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