Antimutagenic effects of red apple and watermelon juices on cyclophosphamide-induced genotoxicity in mice

Asita,

doi:10.5897/ajb11.756

Cited by 7 publications

(1 citation statement)

References 25 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Grape seed extract acts as a potent antioxidant prevented genotoxicity of bone marrow cells by reducing a total number of aberrant cells and different types of structural chromosomal aberrations caused by mutagen [33]. Also, [34] reported that grape seed inhibits the mutagenic effects of cyclophosphamide and/or other mutagens in rats and mice, both in vitro and/or in vivo.…”

Section: Methodsmentioning

confidence: 98%

Antimutagenic effect of grape seed extracted oil on diazinon induced genotoxicity in albino mice

Al-Attar¹

2018

IJCMG

View full text Add to dashboard Cite

Background: Human health hazard due to exposure to chemical pollutants is a global and chronic problem. The pesticides constitute the primary toxic chemicals in the environment. The present study explored the effects of grape seed oil supplementation on diazinon (DZN) induced chromosomal aberrations and sperm abnormalities in male mice. Materials and methods: The tests were carried out on 35 adult male albino mice (16-20 weeks, weighed 30-35 g). Animals were categorized into seven groups each with five mice; the first group was treated with phos¬phate buffer saline (PBS) as negative control group. The second and third groups were treated daily and orally with two different doses of the DZN (60 and 90 mg/kg body weight) respectively dissolved in corn oil. Animals of fourth, fifth and sixth groups were orally administered grape seed oil at a dose of 2 g/kg body weight, after 6 hours the fourth and fifth groups subjected to DZN at the same dose given to second and third groups. Animals of the seventh group were supplied with corn oil. The treatments were continued for five weeks. Results: The results showed that DZN increased the frequency of both chromatid and chromosome aberrations in bone marrow cells and sperm abnormalities. Treatment with grape seed oil extract showed a significant decrease (P<0.01) on both total abnormal chromosomes and sperms, and there was no significant difference between these groups and negative control in most analyzed parameters. The dose of grape seed oil has significantly minimized the effect of first dose of DZN concerning chromosome structure while it worked in the second dose regarding sperm abnormalities. Conclusions: This finding suggests the protective action of grape seed oil against DZN induced clastogenecity and sperm abnormalities.

show abstract

Section: Methodsmentioning

confidence: 98%

Antimutagenic effect of grape seed extracted oil on diazinon induced genotoxicity in albino mice

Al-Attar¹

2018

IJCMG

View full text Add to dashboard Cite

show abstract

In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage

Casanova

Ariagno

Nigro

et al. 2012

J of Applied Toxicology

View full text Add to dashboard Cite

The present study was carried out to investigate the genotoxicity as well as possible protective activity against damage induced by cyclophosphamide (CP) of the aqueous juice of watercress (Nasturtium officinale, W.T. Aiton) in vivo. Male and female Swiss mice 7-8 weeks old (N = 48) were treated by gavage with 1 g kg(-1) body weight and 0.5 g kg(-1) body weight of watercress juice during 15 consecutive days. Genotoxicity and its possible protective effect were tested by the comet assay in peripheral blood cells and the micronucleus test in bone marrow. In addition, biopsies of the bladder, epididymis and testicles of mice were performed to extend the experimental design. Watercress juice per se did not induce genetic damage according to the comet assay and micronucleus study, exhibiting a protective activity against CP (P < 0.05 and P < 0.001, respectively). The comparative analysis of bladder histological changes obtained in the watercress plus CP group against those treated with CP alone suggests a probable protective effect. Further studies are needed in order to establish the protective role of watercress juice against DNA damage.

show abstract

Effects of Watermelon Powder and l-arginine Supplementation on Azoxymethane-Induced Colon Carcinogenesis in Rats

Glenn

Klarich

Kalaba

et al. 2018

Nutrition and Cancer

View full text Add to dashboard Cite

Diets high in fruits and vegetables may help prevent colorectal cancer (CRC). Watermelon consumption may reduce CRC risk due to its concentration of l-citrulline and its role in endothelial nitric oxide (NO) production. Research suggests that increased NO levels have tumoricidal effects. The purpose of this study was to determine the effects of watermelon powder supplementation on aberrant crypt foci (ACF) formation, precancerous lesions, and expression of genes associated with colon carcinogenesis. Thirty-two male Sprague-Dawley rats were assigned into three groups: control, 0.36% l-arginine, or 0.5% watermelon powder and injected with azoxymethane (15 mg/kg body weight). Both l-arginine and watermelon powder groups exhibited lower total numbers of ACF and high multiplicity ACF (P < 0.01). The watermelon powder group exhibited higher NO levels and lower 8-hydroxyguanosine DNA damage (P < 0.05). Watermelon powder and l-arginine downregulated 8-oxoguanine DNA glycosylase gene expression and upregulated O-methylguanine DNA methyltransferase gene expression (P < 0.05). Cyclooxgenase-2 gene expression was lower for rats fed with watermelon powder (P < 0.05). These results suggest that watermelon powder or l-arginine supplementation may reduce the risk of colon cancer by suppressing ACF formation through lowering oxidative DNA damage and inflammation, modulating DNA repair enzyme expression, and/or enhancing NO production.

show abstract

Antimutagenic effects of red apple and watermelon juices on cyclophosphamide-induced genotoxicity in mice

Cited by 7 publications

References 25 publications

Antimutagenic effect of grape seed extracted oil on diazinon induced genotoxicity in albino mice

Antimutagenic effect of grape seed extracted oil on diazinon induced genotoxicity in albino mice

In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage

Effects of Watermelon Powder and l-arginine Supplementation on Azoxymethane-Induced Colon Carcinogenesis in Rats

Contact Info

Product

Resources

About