Antimutagenic and antioxidant activity of a protein fraction from aerial parts of<i>Urtica dioica</i>

Sotto, Antonella Di; Mazzanti, Gabriela; Savickienė, Nijolė; Staršelskytė, Rasa; Baksenskaite, Vaida; Giacomo, Silvia Di; Vitalone, Annabella

doi:10.3109/13880209.2014.950386

Cited by 14 publications

(10 citation statements)

References 14 publications

(22 reference statements)

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“…After 24 h incubation, the cytotoxicity of the treatment was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay according to previous published methods [25]. The assay was carried out at least in three biological replicates, and in each experiment each concentration was tested in six technical triplicates.…”

Section: Methodsmentioning

confidence: 99%

SPC Liposomes as Possible Delivery Systems for Improving Bioavailability of the Natural Sesquiterpene β-Caryophyllene: Lamellarity and Drug-Loading as Key Features for a Rational Drug Delivery Design

et al. 2018

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The natural sesquiterpene β-caryophyllene (CRY) has been highlighted to possess interesting pharmacological potentials, particularly due to its chemopreventive and analgesic properties. However, the poor solubility of this sesquiterpene in aqueous fluids can hinder its uptake into cells, resulting in inconstant responses of biological systems, thus limiting its application. Therefore, identifying a suitable pharmaceutical form for increasing CRY bioavailability represents an important requirement for exploiting its pharmacological potential. In the present study, the ability of soybean phosphatidylcholine (SPC) liposomes to improve bioavailability and absorption of CRY in cancer cells has been evaluated. Liposomal formulations of CRY, differing for lamellarity (i.e., unilamellar and multilamellar vesicles or ULV and MLV) and for the drug loading (i.e., 1:0.1, 1:0.3 and 1:0.5 mol/mol between SPC and CRY) were designed with the aim of maximizing CRY amount in the liposome bilayer, while avoiding its leakage during storage. The low-loaded formulations significantly potentiated the antiproliferative activity of CRY in both HepG2 and MDA-MB-468 cells, reaching a maximum IC50 lowering (from two to five folds) with 1:0.3 and 1:0.1 SPC/CRY MLV. Conversely, increasing liposome drug-loading reduced the ability for CRY release, likely due to a possible interaction between SPC and CRY that affects the membrane properties, as confirmed by physical measures.

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Section: Methodsmentioning

confidence: 99%

SPC Liposomes as Possible Delivery Systems for Improving Bioavailability of the Natural Sesquiterpene β-Caryophyllene: Lamellarity and Drug-Loading as Key Features for a Rational Drug Delivery Design

et al. 2018

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“…Several studies have established that extracts of U. dioica possess various pharmacological effects [37,38,39], including anti-inflammatory [40,41,42], anti-oxidant [43,44,45,46,47], anti-microbial [46,47,48,49], anti-diabetic [39,50,51], cardiovascular [39], anti-ulcer, analgesic [36], immuno-modulatory [35,52], anti-mutagenic [44] and anti-cancer properties. Moreover, this edible plant has considerable chemopreventive capacities and disease-preventing effects on animals and humans.…”

Section: Phytochemical Investigations and Biological Activities Ofmentioning

confidence: 99%

“…The anti-mutagenic activity of a protein fraction from the aerial parts of U. dioica was demonstrated, via the Ames test in various bacteria strains, against the mutagen 2-aminoanthracene; the anti-mutagenic activity can be due to the inhibition of CYP450-isoenzymes, involved in the 2-aminoanthracene mutagen bioactivation [44].…”

Section: Anticancer Activities Of Urtica Dioicamentioning

confidence: 99%

“…The anti-oxidant and radical scavenging activities of U. dioica were reported by ABTS and superoxide-radical scavenger assays and on analysis of the changes in antioxidant enzymes [43,44,45,46,47]. In mice, the treatment with U. dioica methanolic extract from the aerial parts demonstrated hepatoprotective and nephroprotective activities against cisplatin-induced [74,75] toxicity, most likely due to increasing antioxidant defense mechanisms, in fact, this extract has been shown to increase the activities of catalase (CAT) and superoxide dismutase (SOD) enzymes and the content of glutathione (GSH) [76].…”

Section: Anticancer Activities Of Urtica Dioicamentioning

confidence: 99%

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Therapeutic Perspectives of Molecules from Urtica dioica Extracts for Cancer Treatment

et al. 2019

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A large range of chronic and degenerative diseases can be prevented through the use of food products and food bioactives. This study reports the health benefits and biological activities of the Urtica dioica (U. dioica) edible plant, with particular focus on its cancer chemopreventive potential. Numerous studies have attempted to investigate the most efficient anti-cancer therapy with few side effects and high toxicity on cancer cells to overcome the chemoresistance of cancer cells and the adverse effects of current therapies. In this regard, natural products from edible plants have been assessed as sources of anti-cancer agents. In this article, we review current knowledge from studies that have examined the cytotoxic, anti-tumor and anti-metastatic effects of U. dioica plant on several human cancers. Special attention has been dedicated to the treatment of breast cancer, the most prevalent cancer among women and one of the main causes of death worldwide. The anti-proliferative and apoptotic effects of U. dioica have been demonstrated on different human cancers, investigating the properties of U. dioica at cellular and molecular levels. The potent cytotoxicity and anti-cancer activity of the U. dioica extracts are due to its bioactive natural products content, including polyphenols which reportedly possess anti-oxidant, anti-mutagenic and anti-proliferative properties. The efficacy of this edible plant to prevent or mitigate human cancers has been demonstrated in laboratory conditions as well as in experimental animal models, paving the way to the development of nutraceuticals for new anti-cancer therapies.

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“…Urtica dioica L. (stinging nettle) has been accepted in traditional herbal pharmacy for decreasing blood glucose levels in patients with diabetes in some countries including Iran and Turkey (4,5). In addition to its anti-hyperglycemic effect, some other effects including anti-hyperlipidemia, antioxidant, antiinflammatory and anti-cancer have been reported for nettle (6)(7)(8). According to previous researches, nettle extract may decrease blood glucose via increasing insulin secretion in diabetic rats (5).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Ethanolic Extract of Urtica dioica Leaves on High Levels of Blood Glucose and Gene Expression of Glucose Transporter 2 (Glut2) in Liver of Alloxan-Induced Diabetic Mice

et al. 2015

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Background: Urtica dioica L. (stinging nettle) has been accepted for decreasing blood glucose levels; however, the exact mechanism of its anti-hyperglycemic effect remains to be understood. Objectives: We aimed to examine the effects of ethanolic extract of stinging nettle leaves on blood glucose levels and gene expression of Glucose Transporter 2 (Glut2) in liver of alloxan-induced diabetic mice. Materials and Methods: Twenty-four male Naval medical research institute (NMRI) mice were randomly divided to three groups. The control group received saline (10 mL/kg, intraperitoneally) for eight days, the diabetic group received three days of injections of alloxan (200 mg/kg, intraperitoneally) followed by five days of injections of ethanol (20%), and the diabetic + nettle extract group received alloxan for three days followed by five days of injections of the nettle extract (150 mg/kg). Mice were weighed before and after treatments (on days one and nine). On day nine, mice were sacrificed, blood samples were collected for measuring glucose levels and liver was dissected to examine changes in the Glut2 gene expression with the semi-quantitative reverse-transcription-polymerase chain reaction (RT-PCR) method. Results:The results showed that the nettle extract significantly decreased high levels of blood glucose (P < 0.001). The nettle extract also had a preventive effect on decrease in body weight. In addition, the results showed that the Glut2 gene expression was increased in liver of diabetic mice (P < 0.05) and was significantly prevented by the nettle extract in diabetic + nettle extract group (P < 0.05). Conclusions: It can be concluded that the nettle extract can reduce blood glucose levels in diabetic mice, at least partly, by influencing the Glut2 gene expression in mice liver.

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Antimutagenic and antioxidant activity of a protein fraction from aerial parts ofUrtica dioica

Cited by 14 publications

References 14 publications

SPC Liposomes as Possible Delivery Systems for Improving Bioavailability of the Natural Sesquiterpene β-Caryophyllene: Lamellarity and Drug-Loading as Key Features for a Rational Drug Delivery Design

SPC Liposomes as Possible Delivery Systems for Improving Bioavailability of the Natural Sesquiterpene β-Caryophyllene: Lamellarity and Drug-Loading as Key Features for a Rational Drug Delivery Design

Therapeutic Perspectives of Molecules from Urtica dioica Extracts for Cancer Treatment

The Effect of Ethanolic Extract of Urtica dioica Leaves on High Levels of Blood Glucose and Gene Expression of Glucose Transporter 2 (Glut2) in Liver of Alloxan-Induced Diabetic Mice

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