Antimutagenic activities of naturally occurring polyamines in Chinese hamster ovary cells in vitro

Cozzi, Renata; Perticone, Paolo; Bona, Roberta; Polani, Stefania

doi:10.1002/em.2850180308

Cited by 12 publications

(2 citation statements)

References 19 publications

(4 reference statements)

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“…Table I. Table I. compounds (Cozzi et al, 1991;Musk et al, 1997). Several natural compounds and antioxidant agents, such as vanillin (Sasaki et al, 1987a(Sasaki et al, ,b, 1990, garlic acid (Knasmüller et al, 1989), humic acid (Cozzi et al, 1993), fatty acids (Sasaki et al, 1994), squalene (Fan et al, 1996) and Tochu tea (Nakamura et al, 1997), have shown antimutagenic properties against chromosomal damage in CHO cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Araújo

Dias²,

Kronka

et al. 1999

Genet. Mol. Biol.

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Cell culture and treatmentsChinese hamster ovary cells (CHO-9 cell line) were kindly supplied by Prof. A.T. Natarajan (University of Leiden, The Netherlands). Cells were maintained as monolayers growing at 37°C in 25-cm 2 flasks (Corning) containing HAM-F10 (Sigma) plus DMEM (Dulbecco's modi- ABSTRACT Naturally occurring antioxidants have been extensively studied for their capacity to protect organisms and cells from oxidative damage. Many plant constituents including turmeric and curcumin appear to be potent antimutagens and antioxidants. The effects of turmeric and curcumin on chromosomal aberration frequencies induced by the radiomimetic agent bleomycin (BLM) were investigated in Chinese hamster ovary (CHO) cells. Three concentrations of each drug, turmeric (100, 250 and 500 µg/ ml) and curcumin (2.5, 5 and 10 µg/ml), were combined with BLM (10 µg/ml) in CHO cells treated during the G 1 /S, S or G 2 /S phases of the cell cycle. Neither turmeric nor curcumin prevented BLM-induced chromosomal damage in any phases of the cell cycle. Conversely, a potentiation of the clastogenicity of BLM by curcumin was clearly observed in cells treated during the S and G 2 /S phases. Curcumin was also clastogenic by itself at 10 µg/ml in two protocols used. However, the exact mechanism by which curcumin produced clastogenic and potentiating effects remains unknown.

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Section: Discussionmentioning

confidence: 99%

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Araújo

Dias²,

Kronka

et al. 1999

Genet. Mol. Biol.

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“…The present study addressed the question of whether skin fibroblasts derived from Gy\Y mice exhibit increased sensitivity to oxidative stress and UV irradiation because of their lack of spermine. Studies in itro have shown that spermine can protect DNA against hydrodynamic shear [44], heat denaturation [45], UV-C irradiation [46], singlet oxygen [47,48] and other free radicals [8]. We were therefore surprised to find that Gy\Y cells, which have no detectable spermine, were more resistant to H # O # exposure than their normal counterparts.…”

Section: Discussionmentioning

confidence: 99%

Skin fibroblasts from spermine synthase-deficient hemizygous gyro male (Gy/Y) mice overproduce spermidine and exhibit increased resistance to oxidative stress but decreased resistance to UV irradiation

Nilsson

Gritli-Linde

Heby

2000

Biochemical Journal

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Hemizygous gyro male (Gy/Y) mice are a model for X-linked hypophosphataemic rickets. As in humans, the disease is caused by deletions in the Phex gene, a phosphate-regulating gene having homologies with endopeptidases on the X chromosome. Some phenotypic abnormalities in Gy/Y mice have recently been attributed to the fact that the Gy deletion also includes the neighbouring spermine synthase gene, resulting in spermine deficiency. Spermine and its precursors spermidine and putrescine are essential for cell growth and differentiation. As a novel method for studying the function of spermine, we established primary cultures of skin fibroblasts from hemizygous Gy/Y mice. The Gy/Y cells contained no detectable spermine. In view of the fact that spermine is a free-radical scavenger in vitro, we were surprised to find that Gy/Y cells were more resistant to oxidative stress than their normal (X/Y) counterparts. However, our finding that spermidine accumulates markedly in the spermine-deficient Gy/Y cells can probably explain this increased resistance. It is the first indication that spermidine can serve as a free-radical scavenger in vivo and not only in vitro. When subjecting the Gy/Y cells to UV-C irradiation we made another interesting finding: the mutant cells were more sensitive than the normal X/Y cells. This finding indicates that spermine, probably because of its high-affinity binding to DNA, is important in protection against chromatin damage.

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Effects of spermine on formation of HGPRT⁻ mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C in V79 chinese hamster cells

Fiorio¹,

Vellosi²,

Bronzetti³

1994

Environ and Mol Mutagen

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Spermine is a polyamine found in bacteria, animal, and plant tissues. It is involved in a variety of biological processes, and its interaction with DNA stabilizes the secondary structure of the double helix. Spermine is one of the first reported antimutagens, reducing the mutation rate in several prokaryotic test systems, while in eukaryotic organisms conflicting results have been obtained. In light of the significant antimutagenic effect of spermine, it is important to evaluate its activity in mammalian cells in culture. The present study was undertaken to evaluate the ability of spermine to suppress the level of HGPRT- mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C. Spermine reduced the mutation frequency induced by ethylmethanesulfonate and methylmethanesulfonate but did not affect survival; with mitomycin C survival was reduced but mutation rate was not influenced.

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Antimutagenic activities of naturally occurring polyamines in Chinese hamster ovary cells in vitro

Cited by 12 publications

References 19 publications

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Skin fibroblasts from spermine synthase-deficient hemizygous gyro male (Gy/Y) mice overproduce spermidine and exhibit increased resistance to oxidative stress but decreased resistance to UV irradiation

Effects of spermine on formation of HGPRT⁻ mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C in V79 chinese hamster cells

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Antimutagenic activities of naturally occurring polyamines in Chinese hamster ovary cells in vitro

Cited by 12 publications

References 19 publications

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Skin fibroblasts from spermine synthase-deficient hemizygous gyro male (Gy/Y) mice overproduce spermidine and exhibit increased resistance to oxidative stress but decreased resistance to UV irradiation

Effects of spermine on formation of HGPRT− mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C in V79 chinese hamster cells

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Effects of spermine on formation of HGPRT⁻ mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C in V79 chinese hamster cells