Antimikrobielle Wirksamkeit einer Silberbeschichtung von Hydrogellinsen

Nissen, S.E.; Furkert, Franz H.

doi:10.1007/s003470070054

Cited by 20 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibacterial properties of silver coating on contact lens have been widely studied, but the material can leak out of the lenses and its antimicrobial effect fades over time. [ 12 ] Martinez‐Gutierrez et al. [ 13 ] reported the use of silver nanoparticle against various microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Novel Antimicrobial Coating on Silicone Contact Lens Using Glycidyl Methacrylate and Polyethyleneimine Based Polymers

Pillai

Reghu

Vikhe

et al. 2020

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Contact lenses are medical devices commonly used to correct refractive errors and to maintain ocular health. Microorganisms such as bacteria that grow on the lens surface cause irritation to the eyes and can even cause loss of vision. In this paper, two different coating strategies are employed to form an efficient antimicrobial coating on contact lenses. In the first method, a presynthesized copolymer of polyethyleneimine‐graft‐polyethylene glycol methacrylate (PEI‐PEGMA) is used and the coated lenses show antimicrobial activity (in vitro) against methicillin‐resistant Staphylococcus aureus (MRSA) bacteria with killing efficacy >99.99% and log reduction of 5.1 and proxy host cell viability of 79%. In the second method, commercially available monomers/polymers such as glycidyl methacrylate (GMA), sulfobetaine methacrylate, and polyethyleneimine are used. A typical formulation consisting of 1% GMA shows antibacterial activity against MRSA with killing efficacy >99.99% and log reduction of 6.3. Proxy host cell viability for the coated lenses is found to be 90% indicating that the coating is nontoxic. Antibacterial coating reported here is very effective in killing gram‐positive bacteria such as MRSA and S. aureus. The second method using commercially available monomers/polymers involving a simple coating procedure is also easily scalable.

show abstract

Section: Introductionmentioning

confidence: 99%

Novel Antimicrobial Coating on Silicone Contact Lens Using Glycidyl Methacrylate and Polyethyleneimine Based Polymers

Pillai

Reghu

Vikhe

et al. 2020

Macromol. Rapid Commun.

View full text Add to dashboard Cite

show abstract

“…Possible adverse effects of locally released silver include argyria, the graying of tissue associated with silver ion exposure to light, increased incidence of bacterial resistance to silver via efflux pumps [24], lack of efficacy due to dose formulation issues, and local cell toxicity. Development of a silver-based antibiotic contact lens formulation requires sustained release of silver from the contact lens material sufficient to inhibit bacterial proliferation, while having minimal adverse effects on several different ocular cells [25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Minimal In Vitro Antimicrobial Efficacy and Ocular Cell Toxicity from Silver Nanoparticles

2007

View full text Add to dashboard Cite

Silver in various forms has long been recognized for antimicrobial properties, both in biomedical devices and in eyes. However, soluble drugs used on the ocular surface are rapidly cleared through tear ducts and eventually ingested, resulting in decreased efficacy of the drug on its target tissue and potential concern for systemic side effects. Silver nanoparticles were studied as a source of anti-microbial silver for possible controlled-release contact lens controlled delivery formulations. Silver ion release over a period of several weeks from nanoparticle sources of various sizes and doses in vitro was evaluated in vitro against Pseudomonas aeruginosa strain PA01. Mammalian cell viability and cytokine expression in response to silver nanoparticle exposure is evaluated using corneal epithelial cells and eye-associated macrophages cultured in vitro in serum-free media. Minimal microcidal and cell toxic effects were observed for several silver nanoparticle suspensions and aqueous extraction times for bulk total silver concentrations commensurate with comparative silver ion (e.g., Ag(+) ((aq))) toxicity. This indicates that (1) silver particles themselves are not microcidal under conditions tested, and (2) insufficient silver ion is generated from these particles at these loadings to produce observable biological effects in these in vitro assays. If dosing allows substantially increased silver particle loading in the lens, the bactericidal efficacy of silver nanoparticles in vitro is one possible approach to limiting bacterial colonization problems associated with extended-wear contact lenses.

show abstract

“…94 Silver ions interact with the DNA of bacteria preventing cell reproduction, disturb bacterial cell permeability and respiration, and create defects in the bacterial cell wall, resulting in loss of cell contents and cell death. 94 Nissen and Furkert 95 has shown that nonionic, 55% water, hydrophilic lenses with a silver layer decreased the number of adhered P. aeruginosa organisms by four logs and S. aureus organisms by one log compared with uncoated lenses. In an attempt to decrease case contamination, a silver impregnated lens case has been available in the United Kingdom since 2004 and cleared by the United States Food and Drug Administration in 2005 under the name PRO-GUARD.…”

Section: Prevention Of Contaminationmentioning

confidence: 99%

Microbial Contamination of Contact Lenses, Lens Care Solutions, and Their Accessories: A Literature Review

Szczotka-Flynn

Pearlman

Ghannoum

2010

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

207

155

View full text Add to dashboard Cite

Purpose A contact lens (CL) can act as a vector for microorganisms to adhere to and transfer to the ocular surface. Commensal microorganisms that uneventfully cohabitate on lid margins and conjunctivae and potential pathogens that are found transiently on the ocular surface can inoculate CLs in vivo. In the presence of reduced tissue resistance, these resident microorganisms or transient pathogens can invade and colonize the cornea or conjunctiva to produce inflammation or infection. Methods The literature was reviewed and used to summarize the findings over the last 30 years on the identification, enumeration, and classification of microorganisms adherent to CLs and their accessories during the course of normal wear and to hypothesize the role that these microorganisms play in CL infection and inflammation. Results Lens handling greatly increases the incidence of lens contamination, and the ocular surface has a tremendous ability to destroy organisms. However, even when removed aseptically from the eye, more than half of lenses are found to harbor microorganisms, almost exclusively bacteria. Coagulase-negative Staphylococci are most commonly cultured from worn lenses; however, approximately 10% of lenses harbor Gram-negative and highly pathogenic species, even in asymptomatic subjects. In storage cases, the incidence of positive microbial bioburden is also typically greater than 50%. All types of care solutions can become contaminated, including up to 30% of preserved products. Conclusions The process of CL-related microbial keratitis and inflammation is thought to be preceded by the presence or transfer or both of microorganisms from the lens to the ocular surface. Thus, this detailed understanding of lens-related bioburden is important in the understanding of factors associated with infectious and inflammatory complications. Promising mechanisms to prevent bacterial colonization on lenses and lens cases are forthcoming, which may decrease the incidence of microbially driven CL complications.

show abstract

Antimikrobielle Wirksamkeit einer Silberbeschichtung von Hydrogellinsen

Cited by 20 publications

References 0 publications

Novel Antimicrobial Coating on Silicone Contact Lens Using Glycidyl Methacrylate and Polyethyleneimine Based Polymers

Novel Antimicrobial Coating on Silicone Contact Lens Using Glycidyl Methacrylate and Polyethyleneimine Based Polymers

Minimal In Vitro Antimicrobial Efficacy and Ocular Cell Toxicity from Silver Nanoparticles

Microbial Contamination of Contact Lenses, Lens Care Solutions, and Their Accessories: A Literature Review

Contact Info

Product

Resources

About