Antimicrobial, toxicity, and anti-inflammatory activities of Buddleja perfoliata Kunth

Cuevas-Cianca, Sofía Isabel; Lorenzo-Leal, Ana C.; Hernández, Lucina; Sánchez‐Arreola, Eugenio; Bach, Horacio

doi:10.1016/j.phyplu.2022.100357

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…The phytoconstituents of K. fedtschenkoi extracts were identified using a Varian CP-3800 gas chromatograph coupled to a Varian 1200 quadrupole mass spectrometer [ 77 ]. Samples were injected into a Factor Four (VF-5 ms, 30 m × 0.25 mm, 0.25 μm thickness) capillary column.…”

Section: Methodsmentioning

confidence: 99%

Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts

Mejía-Méndez

Bach

Lorenzo-Leal

et al. 2023

Plants

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In this study, the leaves of Kalanchoe fedtschenkoi were consecutively macerated with hexane, chloroform, and methanol. These extracts were used to assess the bioactivities of the plant. The antimicrobial activity was tested against a panel of Gram-positive and -negative pathogenic bacterial and fungal strains using the microdilution method. The cytotoxicity of K. fedtschenkoi extracts was investigated using human-derived macrophage THP-1 cells through the MTT assay. Finally, the anti-inflammatory activity of extracts was studied using the same cell line by measuring the secretion of IL-10 and IL-6. The phytoconstituents of hexane and chloroform extracts were evaluated using gas chromatography–mass spectrometry (GC/MS). In addition, high-performance liquid chromatography (HPLC) was used to study the phytochemical content of methanol extract. The total flavonoid content (TFC) of methanol extract is also reported. The chemical composition of K. fedtschenkoi extracts was evaluated using Fourier-transform infrared spectroscopy (FTIR). Results revealed that the chloroform extract inhibited the growth of Pseudomonas aeruginosa at 150 μg/mL. At the same concentration, methanol extract inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). Regarding their cytotoxicity, the three extracts were highly cytotoxic against the tested cell line at IC50 < 3 μg/mL. In addition, the chloroform extract significantly stimulated the secretion of IL-10 at 50 μg/mL (p < 0.01). GC/MS analyses revealed that hexane and chloroform extracts contain fatty acids, sterols, vitamin E, and triterpenes. The HPLC analysis demonstrated that methanol extract was constituted by quercetin and kaempferol derivatives. This is the first report in which the bioactivities and chemical profiles of K. fedtschenkoi are assessed for non-polar and polar extracts.

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Section: Methodsmentioning

confidence: 99%

Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts

Mejía-Méndez

Bach

Lorenzo-Leal

et al. 2023

Plants

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“…The extracts' chemical profiles of T. vanhouttei were established using a Varian CP-3800 gas chromatograph coupled to a Varian 1200 quadrupole mass spectrometer. The extracts were analyzed according to published protocols [55]. Briefly, 1 µL of samples prepared at 1% (w/v) chloroform were injected into a Factor Four capillary column: VF-5MS (5% phenylmethyl polysiloxane-95% polydimethylsiloxane; Agilent Technologies), 30 m × 0.25 mm, and 0.25 µm thickness.…”

Section: Analysis Of Phytoconstituents By Gc-msmentioning

confidence: 99%

Antimicrobial, Cytotoxic, and Anti-Inflammatory Activities of Tigridia vanhouttei Extracts

Mejía-Méndez,

Lorenzo-Leal,

Bach

et al. 2023

Plants

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In this work, bulb extracts of Tigridia vanhouttei were obtained by maceration with solvents of increasing polarity. The extracts were evaluated against a panel of pathogenic bacterial and fungal strains using the minimal inhibitory concentration (MIC) assay. The cytotoxicity of the extracts was tested against two cell lines (THP-1 and A549) using the MTT assay. The anti-inflammatory activity of the extracts was evaluated in THP-1 cells by measuring the secretion of pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines by ELISA. The chemical composition of the extracts was recorded by FTIR spectroscopy, and their chemical profiles were evaluated using GC-MS. The results revealed that only hexane extract inhibited the growth of the clinical isolate of Pseudomonas aeruginosa at 200 μg/mL. Against THP-1 cells, hexane and chloroform extracts were moderately cytotoxic, as they exhibited LC50 values of 90.16, and 46.42 μg/mL, respectively. Treatment with methanol extract was weakly cytotoxic at LC50 443.12 μg/mL against the same cell line. Against the A549 cell line, hexane, chloroform, and methanol extracts were weakly cytotoxic because of their LC50 values: 294.77, 1472.37, and 843.12 μg/mL. The FTIR analysis suggested the presence of natural products were confirmed by carboxylic acids, ketones, hydroxyl groups, or esters. The GC-MS profile of extracts revealed the presence of phytosterols, tetracyclic triterpenes, multiple fatty acids, and sugars. This report confirms the antimicrobial, cytotoxic, and anti-inflammatory activities of T. vanhouttei.

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