Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment

Obe, Tomi; Nannapaneni, Ramakrishna; Schilling, Wes; Zhang, L.; Kiess, Aaron S.

doi:10.1016/j.japr.2021.100195

Cited by 17 publications

(23 citation statements)

References 66 publications

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“…Also, similar findings were reported by Zishiri et al [45] and Beshiru et al [25]. All isolates by Obe et al [66] were resistant to multiple antibiotics, similar to our findings. In addition, 64% of Obe et al [66] isolates exhibited resistance to aminoglycosides and beta-lactams, which was way higher than our study's.…”

Section: Plos Onesupporting

confidence: 93%

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Identification and characterization of MDR virulent Salmonella spp isolated from smallholder poultry production environment in Edo and Delta States, Nigeria

et al. 2023

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Salmonella is responsible for some foodborne disease cases worldwide. It is mainly transmitted to humans through foods of animal origin through the consumption of poultry products. The increased international trade and the ease of transboundary movement could propel outbreaks of local origin to translate into severe global threats. The present study aimed to characterize Salmonella serovars isolated from poultry farms in Edo and Delta States, Nigeria. A total of 150 samples (faecal, water and feed) were collected from ten poultry farms between January and August 2020 and analyzed for Salmonella characterization using standard bacteriological and molecular methods. Salmonella serovars identified include: Salmonella Enteritidis [n = 17 (39.5%)], Salmonella Typhimurium [n = 13 (30.2%)] and other Salmonella serovars [n = 13 (30.2%)]. All Salmonella serovars were cefotaxime and ampicillin resistant. The presence of the invA gene ranged from 9(69.2%) to 15(88.2%). The spvC gene ranged from 2(14.4%) to 10(58.8%). All Salmonella serovars had sdiA gene. The Salmonella isolates produced some extracellular virulence factors (such as protease, lipase, β-hemolytic activity, and gelatinase), while 13(30.2%) of the overall isolates formed strong biofilms. In conclusion, the detection of multiple antibiotic-resistant Salmonella serovars in faecal sources, which also exhibited virulence determinants, constituted a public health risk as these faecal samples have the potential as manure in the growing of crops. These pathogens can be transmitted to humans nearby and through poultry products, resulting in difficult-to-treat infections and economic loss.

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Section: Plos Onesupporting

confidence: 93%

“…All isolates by Obe et al [66] were resistant to multiple antibiotics, similar to our findings. In addition, 64% of Obe et al [66] isolates exhibited resistance to aminoglycosides and beta-lactams, which was way higher than our study's. A lower MDR of 52.3% compared to our study was reported previously [67].…”

Section: Plos Onesupporting

confidence: 91%

Identification and characterization of MDR virulent Salmonella spp isolated from smallholder poultry production environment in Edo and Delta States, Nigeria

et al. 2023

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“…Some studies have suggested that the presence of organic matter in food-processing environments can reduce the effectiveness of antimicrobial agents, resulting in pathogens being exposed to sublethal concentrations of these agents [21,36,37]. In our study, all the isolates with high chlorine tolerance (F1R13/7, F3R13/1, F2R21, and F4R15/3) were recovered from ready-to-eat salads in which chlorine is often used to disinfect vegetables.…”

Section: Discussionmentioning

confidence: 70%

“…using the broth microdilution method in 96well polystyrene microtiter plates, as described by Obe et al[21], with minor alterations. Colonies of each Acinetobacter spp.…”

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confidence: 99%

Tolerance of foodborne Acinetobacter spp. to sanitizer agents

Fernandes

Ramos

Malta

et al. 2022

J Infect Dev Ctries

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Introduction: The bacteria Acinetobacter spp. are extremely relevant in clinical settings. Recently, they have emerged as potential food-borne opportunistic pathogens. Their ability to form biofilms contributes to antibiotic resistance by generating an environment that facilitates the acquisition and transfer of resistance genes. Studies on the tolerance of Acinetobacter spp. from food sources to sanitizers used in the food industry and homes are necessary to help mitigate food contamination by these microorganisms. Results: The minimum inhibitory (MIC) and bactericidal (MBC) concentrations of NaClO were above 312.5 ppm for all isolates tested and ≥ 2,500 ppm for four isolates from salads. Only three isolates from salads and four isolates from goat milk were inhibited by an MIC lower than 200 ppm of PAA. QAC/BG presented the lowest MIC and MCB values (9.37/6.25 ppm for all isolates tested), suggesting that it is the most effective agent against the isolates used in this study. Conclusions: Our results demonstrate that Acinetobacter spp. isolates from food can be tolerant to the recommended concentrations of NaClO and PAA, which highlights the health risks to consumers.

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“…Biocides aiming to be efficient in eradicating Salmonella in food processing environments will have to overcome the increased expression of efflux pump genes and its high capacity for biofilm formation. It was suggested that biofilm-forming bacteria must also be considered for complete and efficient sanitation of food-contact surfaces besides the planktonic cell targeting [ 135 ]. The biological mechanisms involved in the eradication of Salmonella spp.…”

Section: The Effect Of Biocides Against Foodborne Pathogenic Bacteriamentioning

confidence: 99%

Biocides as Biomedicines against Foodborne Pathogenic Bacteria

et al. 2022

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Biocides are currently considered the first line of defense against foodborne pathogens in hospitals or food processing facilities due to the versatility and efficiency of their chemical active ingredients. Understanding the biological mechanisms responsible for their increased efficiency, especially when used against foodborne pathogens on contaminated surfaces and materials, represents an essential first step in the implementation of efficient strategies for disinfection as choosing an unsuitable product can lead to antibiocide resistance or antibiotic–biocide cross-resistance. This review describes these biological mechanisms for the most common foodborne pathogens and focuses mainly on the antipathogen effect, highlighting the latest developments based on in vitro and in vivo studies. We focus on biocides with inhibitory effects against foodborne bacteria (e.g., Escherichia spp., Klebsiella spp., Staphylococcus spp., Listeria spp., Campylobacter spp.), aiming to understand their biological mechanisms of action by looking at the most recent scientific evidence in the field.

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Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment

Cited by 17 publications

References 66 publications

Identification and characterization of MDR virulent Salmonella spp isolated from smallholder poultry production environment in Edo and Delta States, Nigeria

Identification and characterization of MDR virulent Salmonella spp isolated from smallholder poultry production environment in Edo and Delta States, Nigeria

Tolerance of foodborne Acinetobacter spp. to sanitizer agents

Biocides as Biomedicines against Foodborne Pathogenic Bacteria

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