Antimicrobial susceptibility, virulence potential and sequence types associated with Arcobacter strains recovered from human faeces

Pérez-Cataluña, Alba; Tapiol, J.; Benavent, Clara; Sarvisé, Carolina; Gómez, Frederic; Martinez, Bruno Prata; Terrón-Puig, Margarida; Recio, Gemma; Vilanova, Àngels; Pujol, Isabel; Ballester, Frédéric; Rezusta, Antonio; Figueras, María José

doi:10.1099/jmm.0.000638

Cited by 19 publications

(26 citation statements)

References 45 publications

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“…Additionally, 23 isolates showed resistance to streptomycin, while 16 strains were resistant to ciprofloxacin. This result is also largely consistent with previous studies (Van den Abeele et al, 2016;Pérez-Cataluña et al, 2017;Ferreira et al, 2019). However, previous studies used the disk diffusion method to determine antimicrobial susceptibility.…”

Section: Genetic Prediction Of Antibiotic Resistance and Concordance supporting

confidence: 92%

Genomic Analysis and Antimicrobial Resistance of Aliarcobacter cryaerophilus Strains From German Water Poultry

Müller

Hotzel

Ahlers³

et al. 2020

Front. Microbiol.

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Aliarcobacter cryaerophilus (formerly Arcobacter cryaerophilus) is a globally emerging foodborne and zoonotic pathogen. However, little is known about the species' genomic features and diversity, antibiotic resistance and virulence. In this study, 27 A. cryaerophilus strains from water poultry in Thuringia, Germany, were investigated using whole-genome sequencing. Four of these strains were sequenced using longand short-read sequencing methods to obtain circularized genomes. The German strains belong to the A. cryaerophilus cluster I. Cluster I genomes exhibited a high degree of genetic diversity in which variable sites comprised 9.1% of the core genome. The German strains formed three subgroups that contained 2, 6, and 9 strains, respectively. The genomic analysis of cluster I revealed variable presence of mobile elements and that 65% of the strains lack CRISPR systems. The four circularized genomes carried a ∼2 Mbp chromosome and a single megaplasmid (size 98.1-154.5 Kbp). The chromosome was densely packed with coding sequences (∼92%) and showed inversions and shifts in the gene blocks between different strains. Antimicrobial resistance was assessed using a gradient strip diffusion method and showed that all 27 strains were resistant to cefotaxime and susceptible to erythromycin, gentamicin, and ampicillin. Sixteen strains were also resistant to ciprofloxacin, whereas 23 were resistant to streptomycin. The genetic prediction of antibiotic resistance identified numerous efflux pumps similar to those found in A. butzleri. All strains harbored two beta-lactamase genes which may explain the cefotaxime resistance. A correlation between the gyrA point mutation (Thr-85-Ile) and ciprofloxacin resistance was partially discovered in 15 out of 16 strains. In silico virulence profiling showed a wide range of virulence factors including a full chemotaxis system and most of the flagellar genes. In contrast to A. butzleri, no urease cluster was found. This study provides new insights into the genomic variability of A. cryaerophilus strains of cluster I. The different genetic makeup of these strains may contribute to the virulence of strains and the severity of the infections in humans.

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Section: Genetic Prediction Of Antibiotic Resistance and Concordance supporting

confidence: 92%

Genomic Analysis and Antimicrobial Resistance of Aliarcobacter cryaerophilus Strains From German Water Poultry

Müller

Hotzel

Ahlers³

et al. 2020

Front. Microbiol.

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“…We found hecB, hecA and irgA less frequently and at lower rates as compared to other reports [11,23,25,27,29,31,32]. The least frequently detected gene in our survey was irgA, which is in line with other studies [11,26,27,33], although irgA rates of 25-46% have also been reported [23,25,29,31]. The presence of iroE has rarely been investigated with prevalences of 17-60% [11,23,26,27], which is slightly higher than the detected 13% in our study.…”

Section: Virulence Genessupporting

confidence: 91%

Characterization of Arcobacter strains isolated from human stool samples: results from the prospective German prevalence study Arcopath

et al. 2020

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Background: Arcobacter constitute emerging food-and waterborne pathogens causing gastroenteritis in humans, but the underlying mechanisms are only incompletely understood. We therefore characterized Arcobacter isolates derived from human stool samples that had been collected during a prospective prevalence study in Germany in vitro. Thirty-six bacterial isolates belonging to the species A. butzleri (n = 24), A. cryaerophilus (n = 10) and A. lanthieri (n = 2) were genotyped by ERIC-PCR, the presence of 10 putative virulence genes was assessed and cytotoxic effects on the human intestinal cell line HT-29/B6 were analyzed applying the WST-assay. Results: Genotyping revealed high genetic diversity within the species A. butzleri, A. cryaerophilus and A. lanthieri. Both, A. butzleri and A. lanthieri encoded for a large number of putative virulence genes, while fewer genes were detectable in A. cryaerophilus isolates. Notably, the three cytolethal distending toxin (CDT) genes cdtA, cdtB and cdtC were abundant in both A. lanthieri isolates. Furthermore, all A. butzleri and A. lanthieri, but only one of the A. cryaerophilus isolates exerted cytotoxic effects. Conclusions: Our study provides evidence for the abundance of putative virulence genes in Arcobacter isolates and prominent cytotoxic effects of A. butzleri and A. lanthieri in vitro. The presence of cdtA, cdtB, cdtC in A. lanthieri points towards CDT secretion as potential mechanism underlying cytotoxicity as opposed to A. butzleri. However, the association of the Arcobacter virulence factors detected and human morbidity should be addressed in future studies.

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“…Therefore, for the majority of the cases of bacterial diarrhoea, ciprofloxacin is one of the best treatment options even though more than 10% of the Arcobacter spp. isolates from patients with diarrhoea can be resistant [8]. In the case of our patient, even if she developed symptoms, she would never be treated with this antibiotic, due to her allergy to the fluoroquinolones, and she would probably receive tetracycline instead, due to the fact that the MIC for beta-lactams was elevated.…”

Section: Arcobacter Butzleri and Intestinal Colonizationmentioning

confidence: 71%

“…Since 2017, the Arcobacter genus encompasses a total of 26 species that have been isolated around the planet, mainly from meat and dairy products, but also from vegetables or seafood [5,6]. Species of the Arcobacter genus are the fourth most encountered bacteria that can be isolated from stool cultures (from patients with diarrhea) in countries like Belgium and France [7], for that reason being considered as a new emerging pathogen and as a possible zoonotic pathogen [8]. Four species have been recorded as pathogenic for humans: A. butzleri, A. cryaerophilus, A. thereius and A. skirrowii, and the infection produced by the first one being manifested by acute persistent diarrhea, nausea, vomiting and intestinal rhythm disturbances, although cases of bacteremia not accompanied by diarrhea have been, as well, described [7,[9][10][11].…”

Section: Arcobacter Butzleri and Intestinal Colonizationmentioning

confidence: 99%

Arcobacter butzleri and intestinal colonization

Jiménez‐Guerra¹,

MorenoTorres²,

Moldovan³

et al. 2020

Rev Esp Quimioter

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Antimicrobial susceptibility, virulence potential and sequence types associated with Arcobacter strains recovered from human faeces

Cited by 19 publications

References 45 publications

Genomic Analysis and Antimicrobial Resistance of Aliarcobacter cryaerophilus Strains From German Water Poultry

Genomic Analysis and Antimicrobial Resistance of Aliarcobacter cryaerophilus Strains From German Water Poultry

Characterization of Arcobacter strains isolated from human stool samples: results from the prospective German prevalence study Arcopath

Arcobacter butzleri and intestinal colonization

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