Antimicrobial Susceptibility to Natural Substances of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Isolated from Italian Poultry

Ščerbová, Jana; Lauková, Andrea; Losasso, Carmen; Barco, Lisa

doi:10.1089/fpd.2021.0085

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…isolates from chickens and pigs were multidrug-resistant (MDR), and 99% were resistant to at least one antimicrobial agent (Choi et al, 2021 ). Nalidixic acid, ciprofloxacin, tetracycline, ampicillin, azithromycin, chloramphenicol, and gentamicin were typically the antimicrobials with the highest rate of resistance (Shakir et al, 2021 ; Asuming-Bediako et al, 2022 ; Behailu et al, 2022 ; Ščerbová et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, genomic characterization and antimicrobial resistance of Campylobacter spp. isolates in pets in Shenzhen, China

Bi³

et al. 2023

Front. Microbiol.

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The prevalence of Campylobacter spp.in pets is a potential concern for human health. However, little is known about the pet-related Campylobacter spp. in China. A total of 325 fecal samples were collected from dogs, cats, and pet foxes. Campylobacter spp. were isolated by culture, and MALDI-TOF MS was used to identify 110 Campylobacter spp. isolates in total. C. upsaliensis (30.2%, 98/325), C. helveticus (2.5%, 8/325), and C. jejuni (1.2%, 4/325) were the three found species. In dogs and cats, the prevalence of Campylobacter spp. was 35.0% and 30.1%, respectively. A panel of 11 antimicrobials was used to evaluate the antimicrobial susceptibility by the agar dilution method. Among C. upsaliensis isolates, ciprofloxacin had the highest rate of resistance (94.9%), followed by nalidixic acid (77.6%) and streptomycin (60.2%). Multidrug resistance (MDR) was found in 55.1% (54/98) of the C. upsaliensis isolates. Moreover, 100 isolates, including 88 C. upsaliensis, 8 C. helveticus, and 4 C. jejuni, had their whole genomes sequenced. By blasting the sequence against the VFDB database, virulence factors were identified. In total, 100% of C. upsaliensis isolates carried the cadF, porA, pebA, cdtA, cdtB, and cdtC genes. The flaA gene was present in only 13.6% (12/88) of the isolates, while the flaB gene was absent. By analyzing the sequence against the CARD database, we found that 89.8% (79/88) of C. upsaliensis isolates had antibiotic target alteration in the gyrA gene conferring resistance to fluoroquinolone, 36.4% (32/88) had the aminoglycoside resistance gene, and 19.3% (17/88) had the tetracycline resistance gene. The phylogenetic analysis using the K-mer tree method obtained two major clades among the C. upsaliensis isolates. All eight isolates in subclade 1 possessed the gyrA gene mutation, the aminoglycoside and tetracycline resistance genes, and were phenotypically resistant to six classes of antimicrobials. It has been established that pets are a significant source of Campylobacter spp. strains and a reservoir for them. This study is the first to have documented the presence of Campylobacter spp. in pets in Shenzhen, China. In this study, C. upsaliensis of subclade 1 required additional attention due to its broad MDR phenotype and relatively high flaA gene prevalence.

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Section: Introductionmentioning

confidence: 99%

Prevalence, genomic characterization and antimicrobial resistance of Campylobacter spp. isolates in pets in Shenzhen, China

Bi³

et al. 2023

Front. Microbiol.

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“…Enterocins are another example of bacteriocins, derived from Enterococci spp., which in combination with herbal extracts can be applied as a new ecological approach to Campylobacter reduction in livestock commodities, therefore improving human health safety (Ščerbová et al, 2022 ). Lactobacillus reuteri could also produce inhibitory compounds, under anaerobic fermentation conditions, such as reuterin, which has broad-spectrum antimicrobial activity against various foodborne pathogens (Asare et al, 2020 ).…”

Section: Types Of Probiotics Used Against Campylobacter ...mentioning

confidence: 99%

Anti-Campylobacter Probiotics: Latest Mechanistic Insights

Balta

Butucel

Ştef

et al. 2022

Foodborne Pathogens and Disease

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The Campylobacter genus is the leading cause of human gastroenteritis, with the consumption of contaminated poultry meat as the main route of infection. Probiotic bacteria, such as Lactobacillus , Bacillus , Escherichia coli Nissle, and Bifidobacterium species, have a great immunomodulatory capacity and exhibit antipathogenic effects through various molecular mechanisms. Reducing Campylobacter levels in livestock animals, such as poultry, will have a substantial benefit to humans as it will reduce disease transmissibility through the food chain. Moreover, probiotic-based strategies might attenuate intestinal inflammatory processes, which consequently reduce the severity of Campylobacter disease progression. At a molecular level, probiotics can also negatively impact on the functionality of various Campylobacter virulence and survival factors (e.g., adhesion, invasion), and on the associated colonization proteins involved in epithelial translocation. The current review describes recent in vitro , in vivo , and preclinical findings on probiotic therapies, aiming to reduce Campylobacter counts in poultry and reduce the pathogen's virulence in the avian and human host. Moreover, we focused in particular on probiotics with known anti- Campylobacter activity seeking to understand the biological mechanisms involved in their mode of action.

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Antibiotic resistance in Italian poultry meat production chain: a one-health perspective comparing antibiotic free and conventional systems from the farming to the slaughterhouse

Ferri

Buonavoglia²,

Farooq

et al. 2023

Front. Food. Sci. Technol.

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In this study a multidisciplinary approach was applied in order to determine the diffusion of resistant bacteria and selected antibiotic resistance genes in antibiotic-free and conventional broiler farms. Litter samples coming from the two farming types and surface sponges obtained from carcasses at slaughterhouse level were screened by end-point PCR targeting specific resistance for tetracycline, ampicillin, sulfonamide, aminoglycoside, carbapenem, nitrofurantoin, vancomycin, quinupristin-dalfopristin, lincomycin, linezolid, chloramphenicol molecules. Microbiological investigations were conducted from the carcasses to determine phenotypical and genetic resistance patterns from pathogenic and commensal Gram-negative and Gram-positive strains. At farm level, catA1, sul2, blaTEM and aadA2 genes were amplified in all samples, while from carcasses the most representative genes were sul2, blaTEM, along with the vatD, relative to quinupristin-dalfopristin resistance. Gram-negative isolates included Aeromonas, Salmonella, Proteus spp. And Escherichia coli, while the Gram-positive were represented by Enterococcus strains. Phenotypical and genetic analysis revealed multidrug resistance patterns in Salmonella, E. coli and Serratia isolates, followed by the Enterococcus species. The comparison between antibiotic-free and conventional farming systems showed some difference regarding the distribution of resistance genes at farm level but no significance was obtained comparing the phenotypical resistance profiles of bacterial strains from both groups of samples, suggesting a poor influence of farming model on the diffusion of antibiotic resistance in poultry meat production chain.

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Antimicrobial Susceptibility to Natural Substances of Campylobacter jejuni and Campylobacter coli Isolated from Italian Poultry

Cited by 3 publications

References 22 publications

Prevalence, genomic characterization and antimicrobial resistance of Campylobacter spp. isolates in pets in Shenzhen, China

Prevalence, genomic characterization and antimicrobial resistance of Campylobacter spp. isolates in pets in Shenzhen, China

Anti-Campylobacter Probiotics: Latest Mechanistic Insights

Antibiotic resistance in Italian poultry meat production chain: a one-health perspective comparing antibiotic free and conventional systems from the farming to the slaughterhouse

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