Antimicrobial susceptibility surveillance of obligate anaerobic bacteria in the Kinki area

Shimura, Satoshi; Watari, Hideo; Komatsu, Masaru; Kuchibiro, Tomokazu; Fukuda, Saori; Nishio, Hisaaki; Kita, Machiko; Kida, Kaneyuki; Oohama, Masanobu; Toda, Hirofumi; Nishio, M.; Nishi, Isao; Kimura, Keigo; Sawa, Kana; Fukuda, Nozomi; Kofuku, Tomomi; Nakai, Isako; Niki, Makoto; Ono, Tamotsu; Nakamura, Tatsuya; Wada, Yasunao

doi:10.1016/j.jiac.2019.07.018

Cited by 14 publications

(21 citation statements)

References 18 publications

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“…Of final note, during this study, we came across susceptibility data of other Porphyromonas species also, and it must be concluded that P. asaccharolytica, P. levii , and P. uenonis were less susceptible to antibiotics [ 35 , 66 ]. For instance, two P. asaccharolytica and two P. levii strains from California were resistant to clindamycin (>32 mg/L).…”

Section: Discussionmentioning

confidence: 99%

The Antimicrobial Susceptibility of Porphyromonas gingivalis: Genetic Repertoire, Global Phenotype, and Review of the Literature

et al. 2021

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The in vitro antimicrobial susceptibility of 29 strains of the major periodontal pathogen Porphyromonas gingivalis and three P. gulae (as an ancestor) to nine antibiotics (amoxicillin, amoxicillin/clavulanate, clindamycin, metronidazole, moxifloxacin, doxycycline, azithromycin, imipenem, and cefoxitin) was evaluated by E-testing of minimal inhibitory concentration (MIC) according to international standards. The results were compared with 16 international studies reporting MICs from 1993 until recently. In addition, 77 currently available P. gingivalis genomes were screened for antimicrobial resistance genes. E-testing revealed a 100% sensitivity of P. gingivalis and P. gulae to all antibiotics. This was independent of the isolation year (1970 until 2021) or region, including rural areas in Indonesia and Africa. Regarding studies worldwide (675 strains), several method varieties regarding medium, McFarland inoculation standards (0.5–2) and incubation time (48–168 h) were used for MIC-testing. Overall, no resistances have been reported for amoxicillin + clavulanate, cefoxitin, and imipenem. Few strains showed intermediate susceptibility or resistance to amoxicillin and metronidazole, with the latter needing both confirmation and attention. The only antibiotics which might fail in the treatment of P. gingivalis-associated mixed anaerobic infections are clindamycin, macrolides, and tetracyclines, corresponding to the resistance genes erm(B), erm(F), and tet(Q) detected in our study here, as well as fluoroquinolones. Periodical antibiotic susceptibility testing is necessary to determine the efficacy of antimicrobial agents and to optimize antibiotic stewardship.

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Section: Discussionmentioning

confidence: 99%

The Antimicrobial Susceptibility of Porphyromonas gingivalis: Genetic Repertoire, Global Phenotype, and Review of the Literature

et al. 2021

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“…Therefore we prescribed metronidazole in combination with sulbactam/ampicillin based on the presumption of anaerobic bacteria. It is reported that the susceptibility rate of obligate anaerobes to sulbactam/ampicillin is high ( 24 , 25 ). However, some obligate anaerobes (e.g., Bacteroides species) may show resistance to sulbactam/ampicillin ( 25 , 26 ).…”

Section: Discussionmentioning

confidence: 99%

“…It is reported that the susceptibility rate of obligate anaerobes to sulbactam/ampicillin is high ( 24 , 25 ). However, some obligate anaerobes (e.g., Bacteroides species) may show resistance to sulbactam/ampicillin ( 25 , 26 ). We therefore prescribed metronidazole while being alert for encephalopathy in our case; consequently there was no evidence of antibiotic-associated encephalopathy ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

The First Case Report of Mediastinal Abscess Caused by <i>Gemella bergeri</i>

et al. 2021

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Most cases of mediastinal abscess occur as a postoperative complication of a thoracic surgical procedure or following trauma. The most common causative microorganism is Staphylococcus aureus , but it can be rarely caused by unusual microorganisms, such as Gemella species. These are relatively difficult-to-identify commensal microorganisms of the upper respiratory and gastrointestinal tracts and may cause several infections. A 66-year-old man was diagnosed with Gemella bergeri mediastinal abscess by the molecular detection of bacterial genes. He was successfully treated with penicillin antibiotic for eight weeks. To our knowledge, this is the first case report of mediastinal abscess caused by G. bergeri .

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“…Knowledge of susceptibility patterns to first-line antibiotics and the clinical origin of anaerobic bacteria are necessary to guide empiric therapy choices appropriately and to contribute to the best possible patient outcome. Various studies have shown noticeable variance in the susceptibility to main antibiotic groups between different anaerobic isolates from different countries and very few of these studies document the relevant clinical characteristics of these patients [8,[11][12][13]. In addition, some guidelines such as the European Committee on Antimicrobial Susceptibility Testing (EUCAST) clinical breakpoints lack recommendations for some of the commonly used antibiotics for treatment of anaerobic infections (e.g., moxifloxacin); therefore, interpretation of susceptibility tends to vary between laboratories.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Susceptibility Patterns and Wild-Type MIC Distributions of Anaerobic Bacteria at a German University Hospital: A Five-Year Retrospective Study (2015–2019)

et al. 2020

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Local antimicrobial susceptibility surveys are crucial for optimal empirical therapy guidelines and for aiding in antibiotic stewardship and treatment decisions. For many laboratories, a comprehensive overview of local antimicrobial susceptibility patterns of anaerobic bacteria is still lacking due to the long incubation time and effort involved. The present study investigates the antimicrobial susceptibility patterns and related clinical and demographic data of 2856 clinical isolates of anaerobic bacteria that were submitted for analysis to the Institute for Medical Microbiology and Hygiene of the Freiburg University Medical Center (a tertiary university medical center in Southern Germany) between 2015 and 2019. Antimicrobial susceptibility testing has been carried out according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guideline. Minimum inhibitory concentration (MIC)50 and MIC90 for penicillin, metronidazole, moxifloxacin, and clindamycin were established for Gram-positive anaerobes and for ampicillin-sulbactam, meropenem, metronidazole, moxifloxacin, and clindamycin for Gram-negative anaerobes. The distribution of MIC-values for various antibiotics against anaerobic bacteria was also established, especially for those having no specific breakpoints according to EUCAST guidelines. Most clinically relevant anaerobic bacteria originated from general surgery, neurological, and orthopedic wards. A high proportion of isolates were resistant to moxifloxacin and clindamycin indicating the importance of their susceptibility testing before administration. Based on our study metronidazole and other β-lactam/β-lactamase inhibitor combinations such as ampicillin-sulbactam remain suitable for empirical treatment of infections with anaerobic bacteria.

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Antimicrobial susceptibility surveillance of obligate anaerobic bacteria in the Kinki area

Cited by 14 publications

References 18 publications

The Antimicrobial Susceptibility of Porphyromonas gingivalis: Genetic Repertoire, Global Phenotype, and Review of the Literature

The Antimicrobial Susceptibility of Porphyromonas gingivalis: Genetic Repertoire, Global Phenotype, and Review of the Literature

The First Case Report of Mediastinal Abscess Caused by <i>Gemella bergeri</i>

Antimicrobial Susceptibility Patterns and Wild-Type MIC Distributions of Anaerobic Bacteria at a German University Hospital: A Five-Year Retrospective Study (2015–2019)

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