Antimicrobial susceptibility profile of community acquired and nosocomial isolates of Escherichia coli from clinical blood culture specimens at a Nigerian university teaching hospital

Jombo, Gta; Akpan, S; Epoke, J; Akaa, P. Denen; Eyong, Komomo; An, Gyuse

doi:10.1016/s1995-7645(10)60159-9

Cited by 6 publications

(6 citation statements)

References 32 publications

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“…A similar resistance pattern of bacteria isolates from contact surfaces has been reported (41,42). This result, however, contradicts the findings of Jombo et al (2010), who found that commonly isolated organisms from contact surfaces were susceptible to Amoxicillin. The selection pressure that the antibiotics exert results in increased resistance to βlactam antibiotics (44).…”

Section: Discussioncontrasting

confidence: 92%

A Case Study on Antimicrobial Resistance of Bacterial Isolates From High-Touched Surfaces in Hospitals in Madonna Catholic Hospital, Abia State

2022

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When healthcare professionals handle infected materials with their hands and gloves or when patients come into contact with contaminated surfaces directly, environmental contamination enhances the transmission of bacteria. This study aimed to determine the antimicrobial resistance of bacteria isolated from high-touched surfaces in Madonna Catholic hospital, Abia State. Samples of the most commonly contacted surfaces were gathered by rubbing and spinning sterile swabs sticks with normal saline on the surfaces. Direct inoculation of the swab samples using the streak plate method was done on Blood agar and MacConkey agar plates. The serial dilution method enumerated the bacterial species associated with the contact surfaces. The bacterial isolates were subjected to an antibiotic susceptibility test using the Kirby Bauer disc diffusion technique. This study showed that thirty-six (36) bacteria isolates were obtained from fifty (50) swab samples collected from high-touched surfaces. It was also observed that Staphylococcus aureus is the most frequently occurring isolate with 12 (33.3%), followed by coagulase- negative Staphylococci 10 (27.8%). The least occurring isolate was recorded for Pseudomonas spp. 5 (13.9%). It was recorded in this study that door handles showed the highest distribution of bacterial isolates 14 (38.8%), whereas swab samples obtained from the benches showed a minor distribution 2 (5.5%). However, the antibiotics susceptibility profile of bacterial isolate from the sampled surfaces showed that Ciprofloxacin was the most effective antibiotic tested at a rate of 100%. It was revealed in this study that most of the bacterial isolates showed a highest level of resistance to Chloramphenicol, Tetracycline and Ampiclox (100%). An appreciable number of pathogenic bacteria isolates were detected in the frequently touched surfaces in the hospital. The presence of these organisms could be the leading cause of hospital-acquired infections. Suitable hygienic measures must be adopted at the hospital to reduce the level of contamination and spread of disease through these surfaces. It is also vital to promote excellent personal hygiene like hand washing. This study finding suggests that ciprofloxacin can be used for treatment and control bacterial infections. Stamford Journal of Microbiology, Vol.12 (1) 2022: 1-7

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Section: Discussioncontrasting

confidence: 92%

A Case Study on Antimicrobial Resistance of Bacterial Isolates From High-Touched Surfaces in Hospitals in Madonna Catholic Hospital, Abia State

2022

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“…The isolation of multiresistant mecA positive S. haemolyticus from blood samples (septicaemia) and S. sciuri from various clinical materials (Table 3) in some HCIs clearly indicate that they could be of clinical importance in Nigeria. A number of reports have indicated an increase in the resistance of staphylococci to cotrimoxazole in Nigeria [15,18,21,23,37]. In this study, 72.5% of CNS and 82.3% of S. aureus isolates were resistant to cotrimoxazole.…”

Section: Discussionmentioning

confidence: 47%

“…Although there are reports on the characterization of staphylococci in Nigeria [15-18], many of the studies were limited to antibiotic susceptibility profiles. Moreover, one geographical region or health care institution was investigated, and CNS were not identified to species level.…”

Section: Introductionmentioning

confidence: 99%

Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeria

et al. 2012

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BackgroundThe staphylococci are implicated in a variety of human infections; however, many clinical microbiology laboratories in Nigeria do not identify staphylococci (in particular coagulase negative staphylococci - CNS) to the species level. Moreover, data from multi-centre assessment on antibiotic resistance and epidemiology of the staphylococci are not available in Nigeria. This study investigated 91 non-duplicate staphylococcal isolates obtained from the microbiology laboratories of eight hospitals in Nigeria during the period January to April 2010.MethodsIdentification and antibiotic susceptibility testing was performed using the VITEK 2 system, detection of resistance genes by PCR, and molecular characterization was determined by SCCmec typing, spa and multilocus sequence typing (MLST).ResultsAll the isolates were susceptible to mupirocin, tigecycline, vancomycin and linezolid, but 72.5% of CNS and 82.3% of Staphylococcus aureus were resistant to cotrimoxazole, while multiresistance was observed in 37 of the 40 CNS isolates. Untypeable SCCmec types (ccrC/Class A mec and ccr-negative/Class C2 mec gene complex) in two methicillin-resistant S. aureus (MRSA) were identified. Additionally, ccr-negative/Class A mec and ccr type 4/Class C2 mec gene complex was detected in one isolate each of S. sciuri and S. haemolyticus, respectively. The S. aureus isolates were classified into 21 spa types including two new types (t8987, t9008) among the methicillin-susceptible S. aureus (MSSA) isolates. Two (CC8-SCCmecnon-typeable and CC88-SCCmec IV) and four (CC8-SCCmec III/IV/V; CC30-SCCmec II/III; CC88-SCCmec IV; and ST152-SCCmecnon-typeable) MRSA clones were identified in Maiduguri (North-East Nigeria) and South-West Nigeria, respectively. The proportion of Panton-Valentine leukocidin (PVL)-positive MSSA was high (44.4%) and 56.3% of these strains were associated with sequence type (ST) 152.ConclusionsThe identification of multiresistant mecA positive S. haemolyticus and S. sciuri from clinical samples indicates that characterization of CNS is important in providing information on their diversity and importance in Nigeria. There is the need to develop new SCCmec classification methods for non-typeable methicillin-resistant staphylococci, and to curtail the spread and establishment of the S. aureus ST152 clone in Nigeria. The study presents the first report of a PVL-positive ST152-SCCmecnontypeable MRSA and SCCmec typing of methicillin-resistant CNS in Nigeria.

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“…The bacterium is well characterized and known to have a diverse arsenal of virulence factors that causes a prominent inflammatory response. 2,3 This pathogen affects both immune competent and immuno compromised individuals, frequently resulting in high morbidity and with complications, which constitute problem to health care institutions. 4 S. aureus has been reported by several studies as the causative agent of wide variety of diseases of supportive infections such as boil, wound infection, pustule, subcutaneous and sub-mucosa abscesses, osteomyelitis, mastitis, impetigo, septicemia, meningitis, bronchopneumonia, food poisoning, a common cause of vomiting and diarrhea, and urinary tract infections.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiotic Susceptibility Pattern of Staphylococcus Aureus Isolated from Various Clinical Specimens in South East Nigeria

Nsofor¹

2016

MOJCSR

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Antimicrobial susceptibility profile of community acquired and nosocomial isolates of Escherichia coli from clinical blood culture specimens at a Nigerian university teaching hospital

Cited by 6 publications

References 32 publications

A Case Study on Antimicrobial Resistance of Bacterial Isolates From High-Touched Surfaces in Hospitals in Madonna Catholic Hospital, Abia State

A Case Study on Antimicrobial Resistance of Bacterial Isolates From High-Touched Surfaces in Hospitals in Madonna Catholic Hospital, Abia State

Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeria

Prevalence and Antibiotic Susceptibility Pattern of Staphylococcus Aureus Isolated from Various Clinical Specimens in South East Nigeria

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