Antimicrobial susceptibility patterns of thermophilic Campylobacter spp. from humans, pigs, cattle, and broilers in Denmark

Abstract

The MICs of 16 antimicrobial agents were determined for 202 Campylobacter jejuni isolates, 123 Campylobacter coli isolates, and 6 Campylobacter lari isolates from humans and food animals in Denmark. The C. jejuni isolates originated from humans (75), broilers (95), cattle (29), and pigs (3); the C. coli isolates originated from humans (7), broilers (17), and pigs (99); and the C. lari isolates originated from broilers (5) and cattle (1). All isolates were susceptible to apramycin, neomycin, and gentamicin. Only a few C. jejuni isolates were resistant to one or more antimicrobial agents. Resistance to tetracycline was more common among C. jejuni isolates from humans (11%) than among C. jejuni isolates from animals (0 to 2%). More resistance to streptomycin was found among C. jejuni isolates from cattle (10%) than among those from humans (4%) or broilers (1%). A greater proportion of C. coli than of C. jejuni isolates were resistant to the other antimicrobial agents tested. Isolates were in most cases either coresistant to tylosin, spiramycin, and erythromycin or susceptible to all three antibiotics. More macrolide-resistant isolates were observed among C. coli isolates from swine (79%) than among C. coli isolates from broilers (18%) and humans (14%). Twenty-four percent of C. coli isolates from pigs were resistant to enrofloxacin, whereas 29% of C. coli isolates from humans and none from broilers were resistant. More resistance to streptomycin was observed among C. coli isolates from swine (48%) than among C. coli isolates from broilers (6%) or humans (0%). The six C. lari isolates were susceptible to all antimicrobial agents except ampicillin and nalidixic acid. This study showed that antimicrobial resistance was found only at relatively low frequencies among C. jejuni and C. lari isolates. Among C. coli isolates, especially from swine, there was a high level of resistance to macrolides and streptomycin. Furthermore, this study showed differences in the resistance to antimicrobial agents among Campylobacter isolates of different origins.

Antimicrobial susceptibilities of Campylobacter isolated from food-producing animals on farms (1999–2001): results from the Japanese Veterinary Antimicrobial Resistance Monitoring Program

“…jejuni was isolated mainly from faeces of cattle, broilers and layers whereas C. coli was isolated mainly from faeces of pigs, as has been the case in previous studies in Japan [11], Denmark [19] and Spain [4], indicating that the frequencies of Campylobacter species differ depending on the animal of origin.…”

“…In some previous papers [8,19], the NCCLS breakpoints for other Vertical lines indicate breakpoints for resistance. * * χ 2 (P < 0.01).…”

Prevalence, Molecular Characterization and Antimicrobial Resistance of Thermophilic Campylobacter Isolates from Cattle, Hens, Broilers and Broiler Meat in South-eastern Italy

“…There was a statistically significant difference between the resistance rate to macrolides (erythromycin) of C. coli and C. jejuni isolated in poultry which amounted to 23% and 3% ( p = 0.02). This provided further evidence that the two species express different propensities to become resistant to macrolides (Aarestrup et al, 1997;Ge et al, 2003). There was also a statistically significant difference between the resistance rate to colistin of C. jejuni (48%) and C. coli (15%) in poultry ( p = 0.01).…”

Resistance to three antimicrobial agents of Campylobacter isolated from organically- and intensively-reared chickens purchased from retail outlets

“…Suspected colonies were confirmed using standard criteria, which include growth conditions, colony morphology (flat, glossy, effuse colonies with a tendency to spread), typical characteristics shown under a light microscope at 1000× magnification, negative Gram stain, motility, oxidase test, catalase test and hippurate hydrolysis test [22][23][24][25].…”