Antimicrobial susceptibility pattern in diabetic foot ulcer: A pilot study

Sekhar, Sonal M; Vyas, Navya; Unnikrishnan, M.K.; Rodrigues, G. S.; Mukhopadhyay, Chiranjay

doi:10.4103/2141-9248.141541

Cited by 56 publications

(70 citation statements)

References 8 publications

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“…9 Klebsiella was sensitive to macrolides and aminoglycosides, staphylococcus aureus was sensitive to beta-lactam and macrolides. Proteus was sensitive to quinolones and aminoglycosides, which is similar to the study of Sekhar et al 10…”

Section: Discussionsupporting

confidence: 89%

A Study of Bacteriological Profile and Antimicrobial Spectrum of Diabetic Foot Ulcer

Kumar¹,

M²,

Acharya³

2017

jebmh

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BACKGROUND There are various pathophysiological factors, which along with hyperglycaemic state is responsible for diabetic foot ulcer. Out of these, there are three important components, which play important role that is vascular changes, neuropathy and immune changes. Glycosylation of nerve proteins, endothelial dysfunction and increased lymphocyte apoptosis is the major pathology that leads to above dysfunction. MATERIALS AND METHODS A total of 60 cases were included in this study. For collection of sample, transport culture and sensitivity, we have followed standard operative procedure, samples collected were plated on blood agar, nutrient agar and MacConkey agar. Conventional method was followed for identification of organism isolated from agar plate like Gram staining, motility testing, oxidase testing and catalase testing. RESULTS In our study, out of 60 culture positive patient, we have found that Escherichia coli was 12 in number that is 20%. Klebsiella was found in 18 samples that is 30%. Pseudomonas was isolated in 12 specimen that is 20% and 10% of the wound having staphylococcus aureus infection. Proteus was found in nine specimens. Rest like CONSA, citrobacter and candida was present in one sample each. CONCLUSION The most common organism isolated out of 60 culture positive specimen was Klebsiella that is 30% of the isolate followed by Pseudomonas aeruginosa and Escherichia coli that is 20% each. We have come to conclusion that chloramphenicol, piperacillintazobactam, amikacin and imipenem adequately covered such infection.

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Section: Discussionsupporting

confidence: 89%

A Study of Bacteriological Profile and Antimicrobial Spectrum of Diabetic Foot Ulcer

Kumar¹,

M²,

Acharya³

2017

jebmh

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“…[14,15] But few studies from India have reported a higher prevalence of necrotic cases and polymicrobial infections. [16][17][18] Microbiological evaluation of diabetic foot ulcer infections showed that the prevalence of gram-negative organisms was found to be more than gram-positive organisms. Pseudomonas aerugenosa was the most frequent followed by Klebsiella pneumonia and E.coli.…”

Section: Discussionmentioning

confidence: 99%

Study on bacteriological profile and antibiotic susceptibility pattern in patients with diabetic foot ulcers in a tertiary care teaching hospital

Shareef¹,

Sunny²,

Bhagavan

2018

Journal of Social Health and Diabetes

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Introduction:Diabetic foot ulcer and infections are one of the major complications in diabetic patients leading to frequent hospitalization and increased mortality. Knowledge about the microbes that cause infections will be helpful for providing appropriate antimicrobial therapy. Aim:To evaluate the bacteriological profile of patients with diabetic foot ulcers and their antibiotic susceptibility pattern. Methodology:A cross-sectional study was carried out for a period of eight months in the Department of surgery in patients with diabetic foot ulcer at a tertiary care teaching hospital. Patient data relevant to the study were collected using a standard data collection form designed as per the need of the study. Details of the organisms isolated and susceptibility pattern were collected from microbiology department. Results:A total of 122 pathogens were identified from 71 patients with male (63.38%) predominance over females (36.61%). Out of the 71 patients, 38 (53.52%) patients had monomicrobial infections and 33 (46.47%) patients had polymicrobial infections. Of the total 122 organisms, 79(64.75%) organisms were found to be gram negative organisms and 43(35.24%) were gram positive. Pseudomonas aeruginosa found in 22 (18.03%) patients was the predominant pathogen isolated followed by Klebsiella pneumonia found in 18 (14.75%) patients. The gram-positive organisms isolated showed maximum susceptibility towards antibiotics Teicoplanin and Linezolid while the gram-negative organisms showed susceptibility to Imipenem, Meropenem, and Piperacillin/Tazobactum combination. Conclusion:The study showed a preponderance of gram-negative bacilli among the isolates from the diabetic foot ulcers. It is recommended that antimicrobial sensitivity testing is necessary for initiating appropriate antibiotic regimen which will help to reduce the drug resistance and minimize the healthcare costs.

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“…Although previous studies reported Pseudomonas aeruginosa as the most common isolate in DFU [ 31 , 32 ], many others authors from the late 1990s have shown that Gram positive cocci are the most predominant agents responsible for DFI, with S. aureus being the most commonly isolated pathogen with considerably high rates of MRSA [ 33 , 34 ]. According to our results, most isolates were identified as S. aureus (77.3 %) and 48.7 % of them considered MRSA.…”

Section: Discussionmentioning

confidence: 99%

Molecular typing, virulence traits and antimicrobial resistance of diabetic foot staphylococci

et al. 2016

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BackgroundDiabetes mellitus is a major chronic disease that continues to increase significantly. One of the most important and costly complications of diabetes are foot infections that may be colonized by pathogenic and antimicrobial resistant bacteria, harboring several virulence factors, that could impair its successful treatment. Staphylococcus aureus is one of the most prevalent isolate in diabetic foot infections, together with aerobes and anaerobes.MethodsIn this study, conducted in the Lisbon area, staphylococci isolated (n = 53) from diabetic foot ulcers were identified, genotyped and screened for virulence and antimicrobial resistance traits. Genetic relationship amongst isolates was evaluated by pulsed-field-gel-electrophoresis with further multilocus sequence typing of the identified pulsotypes. PCR was applied for detection of 12 virulence genes and e-test technique was performed to determine minimal inhibitory concentration of ten antibiotics.ResultsAmong the 53 isolates included in this study, 41 Staphylococcus aureus were identified. Staphylococcal isolates were positive for intercellular adhesins icaA and icaD, negative for biofilm associated protein bap and pantone-valentine leucocidin pvl. S. aureus quorum sensing genes agrI and agrII were identified and only one isolate was positive for toxic shock syndrome toxin tst.36 % of staphylococci tested were multiresistant and higher rates of resistance were obtained for ciprofloxacin and erythromycin. Clonality analysis revealed high genomic diversity and numerous S. aureus sequence types, both community- and hospital-acquired, belonging mostly to clonal complexes CC5 and C22, widely diffused in Portugal nowadays.ConclusionsThis study shows that diabetic foot ulcer staphylococci are genomically diverse, present resistance to medically important antibiotics and harbour virulence determinants. These properties suggest staphylococci can contribute to persistence and severity of these infections, leading to treatment failure and to the possibility of transmitting these features to other microorganisms sharing the same niche. In this context, diabetic patients may become a transmission vehicle for microorganisms’ clones between community and clinical environments.

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Antimicrobial susceptibility pattern in diabetic foot ulcer: A pilot study

Cited by 56 publications

References 8 publications

A Study of Bacteriological Profile and Antimicrobial Spectrum of Diabetic Foot Ulcer

A Study of Bacteriological Profile and Antimicrobial Spectrum of Diabetic Foot Ulcer

Study on bacteriological profile and antibiotic susceptibility pattern in patients with diabetic foot ulcers in a tertiary care teaching hospital

Molecular typing, virulence traits and antimicrobial resistance of diabetic foot staphylococci

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