Antimicrobial susceptibility of Trueperella pyogenes isolated from food-producing ruminants

Galán‐Relaño, Ángela; Gómez-Gascón, Lídia; Barrero-Domínguez, Belén; Luque, Inmaculada; Jurado-Martos, Francisco; Vela, Ana Isabel; Sanz-Tejero, Celia; Tarradas, Carmen

doi:10.1016/j.vetmic.2020.108593

Cited by 21 publications

(20 citation statements)

References 25 publications

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“…A special attention was paid to Trueperella pyogenes , as a pathogen that often causes uterine infections and mastitis at dairy farms. In a study conducted by Galán-Relaño et al, [ 3 ] bimodal MIC distribution was detected for oxytetracycline against Trueperella pyogenes and MIC 90 values of 32 µg mL −1 were found out. Another study reported MIC values of oxytetracycline within a wide range between 0.25 to ≥128 µg mL −1 [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

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Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with Trueperella Pyogenes Infection

et al. 2020

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Systemic therapy with oxytetracycline is often used for treatment of clinical metritis although data about its penetration into the uterus and uterine secretion are lacking. Uterine secretions and milk from six cows with clinical metritis were collected for microbiological assay. The animals were treated intramuscularly with long-acting oxytetracycline (20 mg/kg) and samples of plasma, milk and uterine secretions were collected for determination of the antibiotic concentrations by HPLC-PDA analysis. Pharmacokinetics of the antibiotic and in silico prediction of its penetration into the uterus were described. Trueperella pyogenes with MIC values of 16–64 µg mL−1 was isolated (n of cows = 4) from uterine secretions. Oxytetracycline showed fast absorption and penetration in the uterine secretions and milk. No change of withdrawal time for milk was necessitated in cows with clinical metritis. Maximum levels in uterine secretions and predicted concentrations of oxytetracycline in the uterus were lower than MIC values. Systemic administration of long-acting oxytetracycline did not guarantee clinical cure and was not a suitable choice for treatment of clinical metritis associated with Trueperella pyogenes. The appropriate approach to antibiotic treatment of uterine infections of cows requires knowledge on penetration of the antibiotics at the site of infection and sensitivity of pathogens.

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Section: Discussionmentioning

confidence: 99%

“…The costs of treatment and the emergence of resistance to antimicrobial drugs are some serious concerns. Clinical metritis is often associated with mixed infections [ 2 ] and isolation of pathogenic bacteria such as Escherichia coli and Trueperella pyogenes [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with Trueperella Pyogenes Infection

et al. 2020

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“…Zhang reported that T. pyogenes isolated from dairy cows was highly resistant to tetracycline and doxycycline [ 10 ]. High MIC 90 values for oxytetracycline (32 μg/mL), tylosin (64 μg/mL) and erythromycin (1024 μg/mL) have been observed among T. pyogenes isolates from ruminants [ 11 ]. Most T. pyogenes isolates from domestic animals and European bison were nonsusceptible to enrofloxacin and ciprofloxacin [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Luteolin increases susceptibility to macrolides by inhibiting MsrA efflux pump in Trueperella pyogenes

Guo

Huang

et al. 2022

Vet Res

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Trueperella pyogenes (T. pyogenes) is an opportunistic pathogen associated with a variety of diseases in many domestic animals. Therapeutic treatment options for T. pyogenes infections are becoming limited due to antimicrobial resistance, in which efflux pumps play an important role. This study aims to evaluate the inhibitory activity of luteolin, a natural flavonoid, on the MsrA efflux pump and investigate its mechanism. The results of antimicrobial susceptibility testing indicated that the susceptibility of msrA-positive T. pyogenes isolates to six macrolides increased after luteolin treatment, while the susceptibility of msrA-negative isolates showed no change after luteolin treatment. It is suspected that luteolin may increase the susceptibility of T. pyogenes isolates by inhibiting MsrA activity. After 1/2 MIC luteolin treatment for 36 h, the transcription level of the msrA gene and the expression level of the MsrA protein decreased by 55.0–97.7% and 36.5–71.5%, respectively. The results of an affinity test showed that the equilibrium dissociation constant (KD) of luteolin and MsrA was 6.462 × 10–5 M, and hydrogen bonding was predominant in the interaction of luteolin and MsrA. Luteolin may inhibit the ATPase activity of the MsrA protein, resulting in its lack of an energy source. The current study illustrates the effect of luteolin on MsrA in T. pyogenes isolates and provides insight into the development of luteolin as an innovative agent in combating infections caused by antimicrobial-resistant bacteria.

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“…It was formerly classified in the Actinomycetaceae family and was known as Arcanobacterium pyogenes but was reclassified as T. pyogenes according to the 16S rRNA sequence ( Yassin et al, 2011 ). Normally, T. pyogenes is found on the skin and mucous membranes of urogenital tracts, the upper respiratory tract, and udders of healthy animals and is known as an opportunistic pathogen that leads to a variety of infections in animals, including pneumonia, liver abscessation, mastitis, metritis, endocarditis, and osteoarthritis ( Galán-Relaño et al, 2020 ). Infections caused by T. pyogenes often lead to substantial economic losses in livestock industries, and an understanding of how T. pyogenes leads to infection is crucial to reduce these losses.…”

Section: Introductionmentioning

confidence: 99%

TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes

Zhang

et al. 2021

Front. Microbiol.

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TatD DNases are conserved proteins in a variety of organisms and are considered potential virulence factors in Plasmodium falciparum and Streptococcus pneumoniae. However, the function of TatD DNases has not yet been determined in Trueperella pyogenes, which causes various infections in animals and leads to economic losses. In this study, we describe the roles of TatD DNases in T. pyogenes (TpTatDs). A bioinformatics analysis was performed to investigate the sequence characteristics of TpTatDs, and then the ability of recombinant TatD proteins to hydrolyze DNA was determined in the presence of divalent cations. Moreover, we constructed tatD-deficient mutants. The biofilms formed by the wild-type and mutant strains were observed under a microscope. The mortality and bacterial load in the spleen of mice infected with the wild-type strain and tatD-deficient mutants were determined to obtain insights into the role of TatDs in the virulence of T. pyogenes. Two TatD DNases were identified in T. pyogenes. They were Mg2+-dependent DNases and exhibited DNA endonuclease activity. Compared with those formed by the parental strain, biofilms formed by mutants showed a significantly reduced thickness and biomass. Moreover, mutants produced a lower bacterial load in the spleen of mice and compromised virulence. Our data indicated that TatD DNases in T. pyogenes are involved in biofilm formation and required for virulence during infections.

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Antimicrobial susceptibility of Trueperella pyogenes isolated from food-producing ruminants

Cited by 21 publications

References 25 publications

Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with Trueperella Pyogenes Infection

Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with Trueperella Pyogenes Infection

Luteolin increases susceptibility to macrolides by inhibiting MsrA efflux pump in Trueperella pyogenes

TatD DNases Contribute to Biofilm Formation and Virulence in Trueperella pyogenes

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