Antimicrobial susceptibility of Neisseria gonorrhoeae isolates determined by the agar dilution, disk diffusion and Etest methods: comparison of results using GC agar and chocolate agar

Liao, Chun-Hsing; Lai, Chih-Cheng; Hsu, Meng-Shiuan; Chu, Fang-Yeh; Wu, Meiyu; Huang, Yu-Tsung; Hsueh, Po-Ren

doi:10.1016/j.ijantimicag.2010.01.007

Cited by 22 publications

(14 citation statements)

References 15 publications

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“…Compared to the reference Etest, which has previously been shown to correlate well with agar dilution , the MIC Test Strips and M.I.C.Evaluator strips showed an excellent overall essential MIC agreement (range for different antimicrobials) of 99.6% (99.0–100%) and 100%, respectively. The total SIR categorical agreement (range for different antimicrobials) was also high at 88.6% (67.6–100%) and 87.1% (59.8–100%), respectively.…”

Section: Discussionmentioning

confidence: 81%

“…For AMR testing, agar dilution technique for quantitative determination of minimum inhibitory concentration (MIC) of antimicrobials has been the gold standard method. However, particularly when examining lower numbers of isolates the Etest (bioMérieux) is a less laborious, easier, more rapid and effective alternative that produces highly comparable MICs . Etest is unfortunately a relatively expensive test.…”

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confidence: 99%

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Performance characteristics of newer MIC gradient strip tests compared with the Etest for antimicrobial susceptibility testing of Neisseria gonorrhoeae

Jönsson

Jacobsson

Cole

et al. 2018

APMIS

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For Neisseria gonorrhoeae susceptibility testing, Etest, comparable to agar dilution, is frequently used. In recent years, newer MIC gradient strip tests have been commercialized. However, these tests have not been appropriately evaluated for gonococci. We evaluated the sensitivity, specificity, accuracy, quality, availability of antimicrobials and cost of the MIC Test Strip (Liofilchem), M.I.C.Evaluator (Oxoid) and Ezy MIC Strip (HiMedia), compared to the reference Etest (bioMérieux), for gonococcal susceptibility testing. The MICs of eight antimicrobials in 103 gonococcal international reference strains (n = 29) and clinical isolates (n = 74) were examined. Coefficient of determination (R ), complete agreement, essential agreement, SIR categorical agreement, sensitivity, specificity and accuracy were calculated. R of the MICs for the antimicrobials ranged between 0.674-0.996, 0.617-0.993, and 0.643-0.994 for the MIC Test Strip, M.I.C.Evaluator strips and Ezy MIC Strips respectively. The essential agreement (SIR categorical agreement) was 99.6% (88.6%), 100% (87.1%) and 93.0% (83.1%) respectively. M.I.C.Evaluator strips for gonococcal key antimicrobials were lacking and the Ezy MIC Strips showed an inconsistent accuracy, quality and some strips were contaminated. The Liofilchem MIC Test Strips had limitations, but might be relatively accurate alternatives to Etest for gonococci. Strict quality assurance (at manufacturing and testing laboratory), including quality controls, are required.

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Section: Discussionmentioning

confidence: 81%

mentioning

confidence: 99%

Performance characteristics of newer MIC gradient strip tests compared with the Etest for antimicrobial susceptibility testing of Neisseria gonorrhoeae

Jönsson

Jacobsson

Cole

et al. 2018

APMIS

View full text Add to dashboard Cite

show abstract

“…Mean inhibitory concentrations (MICs) were determined by the agar dilution method, and current Clinical and Laboratory Standards Institute cutoffs were used, except for azithromycin, in which we followed the European Committee on Antimicrobial Susceptibility Testing ( http://www.eucast.org ) ( 86 ). A single isolate (from M-2) that did not grow in agar dilution media or GC agar had MICs determined on chocolate agar by Etest: thus, MIC values may be one dilution lower due to enriched agar ( 87 ).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of Neisseria gonorrhoeae during Natural Infection Reveals Differential Expression of Antibiotic Resistance Determinants between Men and Women

Nudel

McClure

Moreau

et al. 2018

mSphere

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Recent emergence of antimicrobial resistance of Neisseria gonorrhoeae worldwide has resulted in limited therapeutic choices for treatment of infections caused by this organism. We performed global transcriptomic analysis of N. gonorrhoeae in subjects with gonorrhea who attended a Nanjing, China, sexually transmitted infection (STI) clinic, where antimicrobial resistance of N. gonorrhoeae is high and increasing. We found that N. gonorrhoeae transcriptional responses to infection differed in genital specimens taken from men and women, particularly antibiotic resistance gene expression, which was increased in men. These sex-specific findings may provide a new approach to guide therapeutic interventions and preventive measures that are also sex specific while providing additional insight to address antimicrobial resistance of N. gonorrhoeae.

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“…Mean inhibitory concentration (MICs) were determined by the agar dilution method and current Clinical and Laboratory Standards Institute cutoffs were used, except for azithromycin, in which we followed the European Committee on Antimicrobial Susceptibility Testing; www.eucast.org (83). A single isolate (from M-2) that did not grow in agar dilution media, nor GC agar, had MICs determined on chocolate agar by E-test, thus MIC values may be one dilution lower due to enriched agar (84).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis ofNeisseria gonorrhoeaeDuring Natural Infection Reveals Differential Expression of Antibiotic Resistance Determinants Between Men and Women

Nudel

McClure

Moreau

et al. 2018

Preprint

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23Neisseria gonorrhoeae is a bacterial pathogen responsible for the sexually transmitted infection, Importance 46Recent emergence of antimicrobial resistance of Neisseria gonorrhoeae worldwide, has resulted 47 in limited therapeutic choices for treating infections caused by this organism. We performed 48 global transcriptomic analysis of N. gonorrhoeae in subjects with gonorrhea who attended the 49 Nanjing (China) STI clinic where antimicrobial resistance of N. gonorrhoeae is high and 50increasing. We found that N. gonorrhoeae transcriptional responses to infection differed in 51 genital specimens taken from men and women, particularly antibiotic-resistance gene expression, 52 which was increased in men. These sex-specific findings may provide a new approach to guide 53 therapeutic interventions and preventive measures that are also sex-specific while also providing 54 additional insight to address antimicrobial resistance of N. gonorrhoeae.

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Antimicrobial susceptibility of Neisseria gonorrhoeae isolates determined by the agar dilution, disk diffusion and Etest methods: comparison of results using GC agar and chocolate agar

Cited by 22 publications

References 15 publications

Performance characteristics of newer MIC gradient strip tests compared with the Etest for antimicrobial susceptibility testing of Neisseria gonorrhoeae

Performance characteristics of newer MIC gradient strip tests compared with the Etest for antimicrobial susceptibility testing of Neisseria gonorrhoeae

Transcriptome Analysis of Neisseria gonorrhoeae during Natural Infection Reveals Differential Expression of Antibiotic Resistance Determinants between Men and Women

Transcriptome Analysis ofNeisseria gonorrhoeaeDuring Natural Infection Reveals Differential Expression of Antibiotic Resistance Determinants Between Men and Women

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