Antimicrobial Susceptibility of &lt;i&gt;Streptococcus gallolyticus&lt;/i&gt; Isolated from Humans and Animals

Nomoto, Ryohei; Tien, Le Hong Thuy; Sekizaki, Tsutomu; Osawa, Ro

doi:10.7883/yoken.66.334

Cited by 14 publications

(8 citation statements)

References 12 publications

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“…Many pathogenic species belonging to the Proteobacteria and Fusobacteria phyla are sensitive to large spectrum β-lactam antibiotics, such as ampicillin and cephalosporins (Stock et al, 2005; Roberts et al, 2006; Poulsen et al, 2012; Nomoto et al, 2013) showing how these antibiotics can drastically improve recovery and mortality rates in malnourished patients (Million et al, 2016). Further, well-designed studies are needed to confirm preliminary results suggesting that cephalosporins, with a broader spectrum on Proteobacteria species, are better than amoxicillin in the routine management of severe acute malnutrition (Trehan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Gut Bacteria Missing in Severe Acute Malnutrition, Can We Identify Potential Probiotics by Culturomics?

et al. 2017

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Severe acute malnutrition is the world-leading cause of children under-five's death. Recent metagenomics studies have established a link between gut microbiota and severe acute malnutrition, describing an immaturity with a striking depletion in oxygen-sensitive prokaryotes. Amoxicillin and therapeutic diet cure most of the children with severe acute malnutrition but an irreversible disruption of the gut microbiota is suspected in the refractory and most severe cases. In these cases, therapeutic diet may be unable to reverse the microbiota alteration leading to persistent impaired development or death. In addition, as enteric sepsis is a major cause of death in this context, identification of missing gut microbes to be tested as probiotics (live bacteria that confer a benefit to the host) to restore rapidly the healthy gut microbiota and prevent the gut pathogenic invasion is of foremost importance. In this study, stool samples of malnourished patients with kwashiorkor and healthy children were collected from Niger and Senegal and analyzed by culturomics and metagenomics. We found a globally decreased diversity, a decrease in the hitherto unknown diversity (new species isolation), a depletion in oxygen-sensitive prokaryotes including Methanobrevibacter smithii and an enrichment in potentially pathogenic Proteobacteria, Fusobacteria and Streptococcus gallolyticus. A complex of 12 species identified only in healthy children using culturomics and metagenomics were identified as probiotics candidates, providing a possible, defined, reproducible, safe, and convenient alternative to fecal transplantation to restore a healthy gut microbiota in malnourished children. Microbiotherapy based on selected strains has the potential to improve the current treatment of severe acute malnutrition and prevent relapse and death by reestablishing a healthy gut microbiota.

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Section: Discussionmentioning

confidence: 99%

Gut Bacteria Missing in Severe Acute Malnutrition, Can We Identify Potential Probiotics by Culturomics?

et al. 2017

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“…Most of the isolates were susceptible to vancomycin, penicillin G and ampicillin, while some were resistant to tetracycline, doxycycline and lincomycin. All the isolates were resistant to tetracycline had tet(M) and/or tet(L) and/or tet(O) genes [65].…”

Section: Streptococcus Speciesmentioning

confidence: 99%

Antibiotic Use in Poultry Production and Its Effects on Bacterial Resistance

Agyare¹,

Boamah²,

Zumbi³

et al. 2019

Antimicrobial Resistance - A Global Threat

186

167

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A surge in the development and spread of antibiotic resistance has become a major cause for concern. Over the past few decades, no major new types of antibiotics have been produced and almost all known antibiotics are increasingly losing their activity against pathogenic microorganisms. The levels of multi-drug resistant bacteria have also increased. It is known that worldwide, more than 60% of all antibiotics that are produced find their use in animal production for both therapeutic and non-therapeutic purposes. The use of antimicrobial agents in animal husbandry has been linked to the development and spread of resistant bacteria. Poultry products are among the highest consumed products worldwide but a lot of essential antibiotics are employed during poultry production in several countries; threatening the safety of such products (through antimicrobial residues) and the increased possibility of development and spread of microbial resistance in poultry settings. This chapter documents some of the studies on antibiotic usage in poultry farming; with specific focus on some selected bacterial species, their economic importance to poultry farming and reports of resistances of isolated species from poultry settings (farms and poultry products) to essential antibiotics.

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“…gallolyticus is susceptible to antimicrobial compounds frequently used for humans. However, previous reports have described resistance to macrolides (45 to 59%) and tetracyclines (56 to 78%) and high-level resistance to aminoglycosides (35 to 43%) (1,(14)(15)(16)42). Therefore, the therapy of S. gallolyticus subsp.…”

Section: Discussionmentioning

confidence: 99%

“…gallolyticus isolates. Resistance to macrolides and tetracyclines and high-level resistance to aminoglycosides are most commonly reported (14)(15)(16). Resistance to glycopeptides has been reported occasionally as a consequence of the acquisition of enterococcal vanA or vanB mechanisms (17)(18)(19).…”

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confidence: 99%

Streptococcus gallolyticus subsp. gallolyticus from Human and Animal Origins: Genetic Diversity, Antimicrobial Susceptibility, and Characterization of a Vancomycin-Resistant Calf Isolate Carrying a vanA -Tn 1546 -Like Element

Romero-Hernández¹,

Tedim²,

Sánchez-Herrero³

et al. 2015

Antimicrob Agents Chemother

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The aim of this work was to characterize the antibiotic susceptibility and genetic diversity of 41 Streptococcus gallolyticus subsp. gallolyticus isolates: 18 isolates obtained from animals and 23 human clinical isolates. Antibiotic susceptibility was determined by the semiautomatic Wider system and genetic diversity by pulsed-field gel electrophoresis (PFGE) with SmaI. Animal isolates grouped separately in the PFGE analysis, but no statistical differences in antimicrobial resistance were found between the two groups. The LMG 17956 sequence type 28 (ST28) strain recovered from the feces of a calf exhibited high levels of resistance to vancomycin and teicoplanin (MIC, >256 mg/liter). Its glycopeptide resistance mechanism was characterized by Southern blot hybridization and a primer-walking strategy, and finally its genome, determined by whole-genome sequencing, was compared with four closely related S. gallolyticus subsp. gallolyticus genomes. Hybridization experiments demonstrated that a Tn1546-like element was integrated into the bacterial chromosome. In agreement with this finding, whole-genome sequencing confirmed a partial deletion of the vanY-vanZ region and partial duplication of the vanH gene. The comparative genomic analyses revealed that the LMG 17956 ST28 strain had acquired an unusually high number of transposable elements and had experienced extensive chromosomal rearrangements, as well as gene gain and loss events. In conclusion, S. gallolyticus subsp. gallolyticus isolates from animals seem to belong to lineages separate from those infecting humans. In addition, we report a glycopeptide-resistant isolate from a calf carrying a Tn1546-like element integrated into its chromosome. N ew taxonomic criteria have recently been applied to the Streptococcus bovis/equinus complex, mainly on the basis of the genetic diversity of the sodA gene, which is considered the best target for adequate identification (1). These taxonomic advances have permitted the study of the epidemiological correlations between particular subspecies and specific human pathologies (2), including gastrointestinal colonization by Streptococcus gallolyticus subsp. gallolyticus (formerly Streptococcus bovis biotype I) and its coincidence with colorectal cancer (3).The rates of colonization by S. gallolyticus subsp. gallolyticus are around 5 to 10% in humans but could be much higher in animals (4). Bacteremia and endocarditis are the main relevant clinical manifestations in humans and in avian species (5, 6). The mortality rate in a broiler flock outbreak was 4.3% (7), and the organism has also been implicated in other veterinary pathologies (8, 9).Similarities and differences between S. gallolyticus subsp. gallolyticus isolates from humans and animals have been described previously (8, 10), including the existence of particular virulent clones with increased invasion and adherence abilities, which favor bloodstream infections (11-13). A recent multilocus sequence typing (MLST)-based study demonstrated a lack of specificity for any particu...

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Antimicrobial Susceptibility of <i>Streptococcus gallolyticus</i> Isolated from Humans and Animals

Cited by 14 publications

References 12 publications

Gut Bacteria Missing in Severe Acute Malnutrition, Can We Identify Potential Probiotics by Culturomics?

Gut Bacteria Missing in Severe Acute Malnutrition, Can We Identify Potential Probiotics by Culturomics?

Antibiotic Use in Poultry Production and Its Effects on Bacterial Resistance

Streptococcus gallolyticus subsp. gallolyticus from Human and Animal Origins: Genetic Diversity, Antimicrobial Susceptibility, and Characterization of a Vancomycin-Resistant Calf Isolate Carrying a vanA -Tn 1546 -Like Element

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