Antimicrobial susceptibility of <i>Pseudomonas aeruginosa</i> isolates from dogs with otitis externa

Mekić, Selma; Matanović, Krešimir; Šeol, Branka

doi:10.1136/vr.d2393

Cited by 61 publications

(62 citation statements)

References 18 publications

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“…These findings were expected and are in agreement with the results of other studies (RIDDLE et al, 2000;TEJEDOR et al, 2003;MEKIĆ et al, 2011).…”

Section: Discussionsupporting

confidence: 83%

“…Compared to this study, a lower susceptibility rate was reported in Croatia a few years ago (MEKIĆ et al, 2011) with one (1.1%) strain susceptible to enrofloxacin. In that study susceptibility testing was performed by Etest ® .…”

Section: Discussionmentioning

confidence: 45%

“…The resistance rate reported in the Croatian study from 2011 (MEKIĆ et al, 2011) was higher (8.7%) but the main reason for that was the difference in the origin of the isolates. A similar difference in susceptibility to ciprofloxacin between isolates of different origin had already been reported by PETERSEN et al (2002).…”

Section: Discussionmentioning

confidence: 85%

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Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

2017

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________________________________________________________________________________________ PINTARIĆ, S., K. MATANOVIĆ, B. ŠEOL MARTINEC:Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs -comparing disk diffusion and microdilution methods. Vet. arhiv 87, 291-300, 2017. ABSTRACTPseudomonas aeruginosa has been determined to be the distinct cause of a number of different infections in both humans and animals. Apart from its high intrinsic resistance, which makes it difficult to treat, it also has a great capacity to acquire further resistance mechanisms. These mechanisms can be present simultaneously in one cell, conferring a multiresistant phenotype. Taking into account that the known risk factor for selection of resistant strains is excessive and inappropriate drug use, especially fluoroquinolones, the aim of this study was to determine the susceptibility of P. aeruginosa isolates to these antibacterial agents. The susceptibility of 90 canine isolates was determined by disk diffusion susceptibility test and the broth microdilution method. Both methods showed that isolates were significantly more sensitive to ciprofloxacin than to marbofloxacin or enrofloxacin, and more sensitive to marbofloxacin than to enrofloxacin. The results of microdilution and disk diffusion testing were in 98.9% agreement for ciprofloxacin and in 91.1% agreement for marbofloxacin, with no statistically significant difference between the two methods (P>0.05) for these two antibiotics. For enrofloxacin, the results of microdilution and disk diffusion testing were in 76.6% agreement, with a statistically significant disagreement between the two methods (P<0.05). In 16 cases, the disk diffusion test overestimated the number of strains susceptible to enrofloxacin compared with the microdilution test. These results suggest that for P. aeruginosa infections in dogs, therapy using enrofloxacin, based on the results of disk diffusion testing, may lead to ineffective treatment and could contribute to selection of resistant and multiresistant strains.

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“…These findings were expected and are in agreement with the results of other studies (RIDDLE et al, 2000;TEJEDOR et al, 2003;MEKIĆ et al, 2011).…”

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 45%

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Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

2017

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“…However, panaminoglycoside resistance e.g., gentamicin, spectinomycin, streptomycin and amikacin has been increasingly reported in the P. aeruginosa dog and cat isolates [18,19,23]. Such resistance has been clarified to be predominantly due to a poorly understood mechanism namely "impermeability resistance" as a consequence of diminished drug uptake and/or accumulation [21,31].…”

mentioning

confidence: 99%

Contribution of the MexXY Multidrug Efflux Pump and Other Chromosomal Mechanisms on Aminoglycoside Resistance in <i>Pseudomonas aeruginosa</i> Isolates from Canine and Feline Infections

Poonsuk

Chuanchuen

2012

J. Vet. Med. Sci.

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ABSTRACT. As study of multidrug efflux pumps is a crucial step for development of efflux pump inhibitors for treatment of Pseudomonas aeruginosa infection, the objective of this study was to examine the contribution of the MexXY multidrug efflux systems and other chromosomal mechanisms in aminoglycoside (AMG) resistance in P. aeruginosa isolated from dogs and cats. Thirteen Pseudomonas aeruginosa isolates from canine and feline infections were examined for contribution of the MexXY multidrug efflux pump and four other chromosomally-encoded genes including PA5471, galU, nuoG and rplY to AMG resistance. All the isolates were resistant to multiple AMGs and expressed mexXY. Deletion of mexXY caused 2-to 16-fold reduction in AMG MICs. Overproduction of MexXY did not fully account for the observed AMG resistance. No good correlations were detected between MexXY transcription level and AMG MICs. While no mutations were found in mexZ, PA5471 expression varied and its impact on MexXY expression and AMG resistance is diverse. No mutations were found in galU. Only two isolates carried a single base change G-367-T in rplY. Complete transcription of nuoG was detected in all the isolates. In conclusion, the MexXY multidrug efflux pump plays a role in AMG resistance in the dog and cat P. aeruginosa isolates, while disruption of nuoG, rplY and galU did not have a significant impact. These results indicate the existence of uncharacterized AMG-resistance mechanisms. Pseudomonas aeruginosa is infamously known as a common cause of chronic and recurrent infections in both humans and animals, of which the most-notably diseases in dogs and cats include otitis externa/media, urinary tract infection and pyoderma [9,10,20]. Treatment of P. aeruginosa infection is challenging, because the pathogen intrinsically exhibits and efficiently develops high resistance to several antimicrobials structurally and functionally unrelated, leading to multidrug resistance [1,24].As many antipseudomonal drugs are available for therapeutic use in dogs and cats, aminoglycosides (AMGs) are considered a vital component of antipseudomonal chemotherapy [22] due to their efficacy, safety and reasonable price. However, panaminoglycoside resistance e.g., gentamicin, spectinomycin, streptomycin and amikacin has been increasingly reported in the P. aeruginosa dog and cat isolates [18,19,23]. Such resistance has been clarified to be predominantly due to a poorly understood mechanism namely "impermeability resistance" as a consequence of diminished drug uptake and/or accumulation [21,31]. The MexXY efflux system, a multidrug efflux pump in the resistance-nodulation-cell-division (RND) family, is involved in the reduced level of AMG accumulation implicated in both impermeability-type and adaptive-type AMG resistance [12,21,22]. The MexXY efflux pump is encoded by the mexXY operon and evidently serves as the major AMG-resistance mediating system in P. aeruginosa clinical isolates. This system additionally confers resistance to nonaminoglycosides, including tetracycline...

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“…However, there is likely a much larger impact from the widespread dissemination of other multidrug-resistant pathogens that are more common causes of infection in pets, such as meticillin-resistant Staphylococcus pseudintermedius (Perreten and others 2010) and, as reported by Mekić and others (2011) in this week's issue of Veterinary Record , multidrug resistant Pseudomonas species.…”

mentioning

confidence: 99%

Antimicrobial resistance: time for action

Weese

2011

Veterinary Record

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Antimicrobial susceptibility of Pseudomonas aeruginosa isolates from dogs with otitis externa

Cited by 61 publications

References 18 publications

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

Contribution of the MexXY Multidrug Efflux Pump and Other Chromosomal Mechanisms on Aminoglycoside Resistance in <i>Pseudomonas aeruginosa</i> Isolates from Canine and Feline Infections

Antimicrobial resistance: time for action

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