Antimicrobial susceptibility of <i>Acinetobacter</i> strains identified by DNA‐DNA hybridization

Tjernberg, Ingela

doi:10.1111/j.1699-0463.1990.tb01039.x

Cited by 14 publications

(8 citation statements)

References 21 publications

(29 reference statements)

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“…baumannii strains were generally more resistant than strains identified as species other than A. baumannii. This is consistent with three recently published reports (8,19,20). Within A. baumannii strains, isolates belonging to biotype 9 were more resistant than other biotypes, confirming previous data (10).…”

Section: Materuils and Methodssupporting

confidence: 93%

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Antimicrobial susceptibility of Acinetobacter species

Seifert

Baginski

Schulze

et al. 1993

Antimicrob Agents Chemother

143

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The in vitro activities of 16 antimicrobial agents against 180 Acinetobacter strains isolated from blood cultures (n = 162), central venous catheters (n = 11), and cerebrospinal fluids (n = 7) were studied. MICs were determined by a microtiter broth dilution method. Considerable differences in antimicrobial drug susceptibility against strains belonging to different species could be demonstrated. Acinetobacter baumannii isolates (n = 108) were generally more resistant than isolates identified as species other than A. baumannii. Multidrug resistance was common among A. baumannii isolates. Of the antimicrobial agents tested, imipenem was highly active against all A. baumannii isolates, and the other agents tested were only moderately active or inactive. Good activity against Acinetobacter species strain 3 was demonstrated for imipenem, amikacin, and ciprofloxacin.Most of the strains belonging to other species were susceptible to imipenem, ciprofloxacin, expanded-spectrum cephalosporins, amoxicillin-clavulanate, and the aminoglycosides but were resistant to ampicillin and older cephalosporins.

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Section: Materuils and Methodssupporting

confidence: 93%

“…Among species other than A. baumannii, strains of Acinetobacter species strains 3 and 10 and A. johnsonii showed the highest resistance. The most susceptible strains were found in the species A. junii and A. Iwoffii; similar data were published recently (19).…”

Section: Materuils and Methodssupporting

confidence: 86%

Antimicrobial susceptibility of Acinetobacter species

Seifert

Baginski

Schulze

et al. 1993

Antimicrob Agents Chemother

143

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“…The proper identification of Acinetobacter spp. at the species level is important for the application of the appropriate therapy to infections, because differences in antimicrobial efficacy against strains belonging to different species have been demonstrated (26,28).…”

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confidence: 99%

Application of a Microsphere-Based Array for Rapid Identification of Acinetobacter spp. with Distinct Antimicrobial Susceptibilities

et al. 2008

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Acinetobacter spp. have emerged as important nosocomial and multidrug-resistant pathogens in the last decade. A. calcoaceticus, A. baumannii, Acinetobacter genospecies 3, and Acinetobacter genospecies 13TU are genetically closely related and are referred to as the A. calcoaceticus-A. baumannii complex (ACB complex). Distinct Acinetobacter spp. may be associated with differences in antimicrobial susceptibility, so it is important to identify Acinetobacter spp. at the species level. We developed a microsphere-based array that combines an allele-specific primer extension assay and microsphere hybridization for the identification of Acinetobacter spp. This assay can discriminate the 13 different Acinetobacter spp. in less than 8.5 h, and it has high specificity without causing cross-reactivity with 14 other common nosocomial bacterial species. The sensitivity of this assay was 100 A. baumannii cells per ml of blood, and it could discriminate multiple species in various mixture ratios. The developed assay could differentiate clinical Acinetobacter spp. isolates with a 90% identification rate. The antimicrobial susceptibility test showed that A. baumannii isolates were resistant to most antimicrobial agents other than imipenem, while the genospecies 3 and 13TU isolates were more susceptible to most antimicrobial agents, especially ciprofloxacin and ampicillinsulbactam. These results supported the idea that this assay possibly could be applied to clinical samples and provide accurate species identification, which might be helpful for clinicians when they are treating infections caused by Acinetobacter spp.

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“…All strains were identified as belonging to the genus Acinetobacter on the basis of following characteristics: oxidase negative, Gram-negative, strictly aerobic, non-motile, cocco bacilli. Identification was done according to the newest terminology by a numerical approach (10, 25) using a simplified identification scheme based on the data in (31). A strain was considered identified if the identification score (normalized likelihooda parameter stating the probability of identification of a strain to one of the species or DNA groups in the identification scheme) was 20.95 and the modal likelihood fraction (a parameter stating the degree of deviation of a strain from the typical reaction pattern of a strain in that species or DNA group) was 20.0001.…”

Section: Phenotypical Identificationmentioning

confidence: 99%

“…The present study was undertaken to eludicate the occurrence of Acinetobacter in the whole of Denmark, including an attempt to identify the isolates according to the newest terminology using a simplified phenotypical identification scheme containing 14 biochemical and susceptibility reactions adopted from Gerner-Smidt et al (16) and Tjernberg (31), and to evaluate the clinical relevance of the newly described different Acinetobacter species. In addition, the antibiotic susceptibility toward 10 antibiotics, including the 5 P-lactam antibiotics proposed by Joly-Guillou et al , was recorded.…”

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confidence: 99%

Acinetobacterin Denmark: I. Taxonomy, antibiotic susceptibility, and pathogenicity of 112 clinical strains

Gerner‐Smidt

Frederiksen

1993

Apmis

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One hundred and twelve clinical strains of Acinetobacter were collected during a 7‐month period in 1990–1991 from Danish clinical microbiology departments. According to the old notation, 37 strains were biovar anitratus and 75 b.lwoffii. Using the newest terminology, 35 strains were unambiguously identified as the species A. haemolyticus, A. johnsonii, A. radioresistens, and as the unnamed phenons 6, 10, and 14, by a numerical approach using a simplified phenotypical identification scheme. Most of the remaining strains were identified to the genotypically heterogeneous A. calcoaceticus‐A. baumannii complex and the A. lwoffii phenotype. Sixteen strains (14%) were left unidentified. Eight A. lwoffii strains were glucose‐oxidizing and were thus classified as b. anitratus using the old terminology. The strains were tested for susceptibility to ampicillin, piperacillin, ticarcillin, cefotaxime, ceftazidime, imipenem, gentamicin, tetracycline, sulphonamide, and nalidixic acid. All strains were susceptible to imipenem. The susceptibility to the remaining β‐lactams was variable, the A. lwoffii isolates being the most and the A. calcoaceticus‐A. baumannii complex strains the least susceptible. All non‐A. calcoaceticus‐A. baumannii complex strains were susceptible to all other antibiotics tested, except for one A. lwoffii strain that was sulphonamide resistant. Twenty‐two percent of the strains in the A. calcoaceticus‐A. baumannii complex showed reduced susceptibility or resistance to gentamicin, but only sporadic resistance to sulphonamides, tetracyclines, and nalidixic acid. Eight infections were recorded: three cases of septicaemia, three cases of peritonitis related to continuous ambulatory peritoneal dialysis, and two cases of recurrent urinary tract infection. No epidemics were detected.

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Antimicrobial susceptibility of Acinetobacter strains identified by DNA‐DNA hybridization

Cited by 14 publications

References 21 publications

Antimicrobial susceptibility of Acinetobacter species

Antimicrobial susceptibility of Acinetobacter species

Application of a Microsphere-Based Array for Rapid Identification of Acinetobacter spp. with Distinct Antimicrobial Susceptibilities

Acinetobacterin Denmark: I. Taxonomy, antibiotic susceptibility, and pathogenicity of 112 clinical strains

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