Antimicrobial susceptibility of Bacillus anthracis strains from Hungary

Kreizinger, Zsuzsa; Sulyok, Kinga M.; Makrai, László; Rónai, Zsuzsanna; Fodor, László; Jánosi, Szilárd; Gyuranecz, Miklós

doi:10.1556/004.2016.015

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…However, no such effects were observed in our experiments. During our literature search, three other antibiotics, rifampicin, daptomycin and linezolid, were also identified as potentially interesting substances (Table 2) which, apparently, can be tolerated by B. anthracis in higher concentrations than its related species (Dabbs et al 1995;Bryskier 2002;Cavallo et al 2002;Mohammed et al 2002;Citron and Appleman 2006;Luna et al 2007;Horii et al 2011;Ikeda et al 2015;Kreizinger et al 2016). During our experiments, these substances were not able to improve CEFOMA selectivity with the tested panel of non-target reference strains.…”

Section: Discussionmentioning

confidence: 88%

“…Not surprisingly, the susceptibility to most antibiotics among the B. cereus sensu lato group was, in general, rather similar. However, in case of the two macrolide antibiotics erythromycin and azithromycin, it was noted that strains of B. anthracis exhibited higher tolerance towards these substances than many other strains of the B. cereus sensu lato group (Yamamoto et al 2001;Bryskier 2002;Cavallo et al 2002;Mohammed et al 2002;Frean et al 2003;Jones et al 2003;Schlegelova et al 2003;Turnbull et al 2004;Citron and Appleman 2006;Luna et al 2007;Ikeda et al 2015;Kreizinger et al 2016). It is important to note that many strains of B. anthracis are defined as being sensitive to erythromycin and azithromycin (Turnbull 2008).…”

Section: Agar Development and Specificity Testingmentioning

confidence: 99%

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Development of a novel selective agar for the isolation and detection of Bacillus anthracis

et al. 2020

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Aims: The aim of this study was to develop a novel selective agar for the specific isolation and detection of Bacillus anthracis. Methods and Results: Based on published data on antibiotic resistance and susceptibility of B. anthracis and other closely related species of the Bacillus cereus sensu lato group, a new selective agar formulation termed CEFOMA (Bacillus CEreus sensu lato group-specific antibiotics, FOsfomycin, MAcrolides) was developed and evaluated. All tested strains of B. anthracis were able to grow on CEFOMA with the same colony number as on non-selective media, whereas CEFOMA inhibited the growth of the other species within the B. cereus sensu lato group. In comparison to other selective agars, CEFOMA had a superior performance and considerably reduced the total amount of accompanying flora in soil. Furthermore, B. anthracis was successfully isolated from deliberately spiked soil samples. Conclusions: CEFOMA is a highly promising selective agar for the efficient isolation of B. anthracis from environmental samples with a large bacterial background flora. Significance and Impact of the Study: The isolation of B. anthracis from environmental samples is severely impaired by the lack of adequate selective agars which suppress the growth of other bacteria. CEFOMA agar represents an important improvement and suitable alternative to currently used selective agars.

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Section: Discussionmentioning

confidence: 88%

Section: Agar Development and Specificity Testingmentioning

confidence: 99%

Development of a novel selective agar for the isolation and detection of Bacillus anthracis

et al. 2020

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“…In order to validate clade-specific SNPs identified by whole genome SNP-discovery, and to determine the distribution of these SNPs in additional B. anthracis DNAs, novel PCR assays were designed and tested. The new assay method is a probe-less real-time qPCR hybrid based on the Melt-MAMA test [20], previously used by us [4,13] or others [21], and an older melt-independent approach employing two separate PCR-reactions to interrogate for alternate allele states, ARMS (Amplification Refractory Mutation System) [22]. Our hybrid method makes use of mismatch (forward) primers featuring an additional mismatch to both alleles at the antepenultimate (-3) position [20] and the allele-specific match/mismatch at the 3'-position like in melt-MAMA and ARMS.…”

Section: Interrogation Of Snps Via Pcr By Relative Ct-value Analysis mentioning

confidence: 99%

Phylogenetic Placement of Isolates Within the Trans-Eurasian Clade A.Br.008/009 of Bacillus anthracis

et al. 2019

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The largest phylogenetic lineage known to date of the anthrax pathogen Bacillus anthracis is the wide-spread, so-called Trans-Eurasian clade systematically categorized as the A.Br.008/009 group sharing two defining canonical single-nucleotide polymorphisms (canSNP). In this study, we genome-sequenced a collection of 35 B. anthracis strains of this clade, derived from human infections, animal outbreaks or soil, mostly from European countries isolated between 1936 and 2008. The new data were subjected to comparative chromosomal analysis, together with 75 B. anthracis genomes available in public databases, and the relative placements of these isolates were determined within the global phylogeny of the A.Br.008/009 canSNP group. From this analysis, we have detected 3754 chromosomal SNPs, allowing the assignation of the new chromosomal sequences to established sub-clades, to define new sub-clades, such as two new Spanish, one Bulgarian or one German group(s), or to introduce orphan lineages. SNP-based results were compared with that of a multilocus variable number of tandem repeat analysis (MLVA). This analysis indicated that MLVA typing might provide additional information in cases when genomics yields identical genotypes or shows only minor differences. Introducing the delayed mismatch amplification assay (DMAA) PCR-analysis, we developed a cost-effective method to interrogate for a set of ten phylogenetically informative SNPs within genomes of A.Br.008/009 canSNP clade strains of B. anthracis. By this approach, additional 32 strains could be assigned to five of ten defined clades.

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Diverging Characteristics – Mercury Resistance and Antibiotic Susceptibility of Bacillus anthracis Isolated from Different Estuarine Environments

Saranya,

Sundaramanickam,

Dineshkumar

et al. 2024

Geomicrobiology Journal

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Antimicrobial susceptibility of Bacillus anthracis strains from Hungary

Cited by 4 publications

References 13 publications

Development of a novel selective agar for the isolation and detection of Bacillus anthracis

Development of a novel selective agar for the isolation and detection of Bacillus anthracis

Phylogenetic Placement of Isolates Within the Trans-Eurasian Clade A.Br.008/009 of Bacillus anthracis

Diverging Characteristics – Mercury Resistance and Antibiotic Susceptibility of Bacillus anthracis Isolated from Different Estuarine Environments

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