Antimicrobial Susceptibility, Heavy Metals Tolerance and Plasmid Curing of &lt;i&gt;Shigella&lt;/i&gt; Species Isolated from El- Dakahlia, Egypt

El-Sherbiny, Gamal M.; Shehata, M E

doi:10.12691/ajmr-2-6-7

Cited by 12 publications

(13 citation statements)

References 34 publications

(28 reference statements)

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“…Moreover, the membrane mimics the extracellular matrix, since gelatin resembles collagen and connective tissue found in the body. [11,40] In addition, epithelial bending is essential for lumen morphogenesis, [41] hence the presented method could be applicable for tissue engineering of hollow organs. One lumen morphogenic pathway is apical constriction by which the epithelial cells undergo apical shrinkage while keeping their volume constant.…”

Section: Stretch Of Cell Culture On a Gelatin Hydrogel Membranementioning

confidence: 99%

“…Depending on the type of polymer, the fabrication method, and the crosslinking method, hydrogels' physical and/or chemical properties can be tuned to suit various fields such as biomedicine, soft electronics, sensors, and actuators. [9][10][11] Hydrogels have a potential use in the field of tissue engineering. Since the microenvironment of in vivo tissues is mechanically flexible, cells are exposed to mechanical forces of various durations, frequencies, and amplitudes.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of thin gelatin hydrogel membranes with balloon properties for dynamic tissue engineering

Jepsen¹,

Nielsen²,

Almdal³

et al. 2018

Biopolymers

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Cell or tissue stretching and strain are present in any in vivo environment, but is difficult to reproduce in vitro. Here, we describe a simple method for casting a thin (about 500 μm) and soft (about 0.3 kPa) hydrogel of gelatin and a method for characterizing the mechanical properties of the hydrogel simply by changing pressure with a water column. The gelatin is crosslinked with mTransglutaminase and the area of the resulting hydrogel can be increased up 13‐fold by increasing the radial water pressure. This is far beyond physiological stretches observed in vivo. Actuating the hydrogel with a radial force achieves both information about stiffness, stretchability, and contractability, which are relevant properties for tissue engineering purposes. Cells could be stretched and contracted using the gelatin membrane. Gelatin is a commonly used polymer for hydrogels in tissue engineering, and the discovered reversible stretching is particularly interesting for organ modeling applications.

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Section: Stretch Of Cell Culture On a Gelatin Hydrogel Membranementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of thin gelatin hydrogel membranes with balloon properties for dynamic tissue engineering

Jepsen¹,

Nielsen²,

Almdal³

et al. 2018

Biopolymers

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show abstract

“…The peak at 1598 cm À1 was attributed to the deformation vibration of amino groups, and the one at 1650 cm À1 was assigned to amide stretching vibrations. 17 From the infrared spectra of CMCS, we could see a strong new peak at 1735 cm À1 representing the carboxylate C¼ ¼O asymmetric stretching. The peak at 1384 cm À1 was assigned to the symmetric stretching vibration of carboxylate C¼ ¼O.…”

Section: Articlementioning

confidence: 93%

Preparation, characterization and the effect of carboxymethylated chitosan–cellulose derivatives hydrogels on wound healing

Fan

Tan

Wang

et al. 2012

J of Applied Polymer Sci

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Oxidized carboxymethyl cellulose (OCMC) was prepared by an oxidation reaction of carboxymethyl cellulose in the presence of sodium periodate. In situ crosslinked hydrogels were obtained through the crosslinking reaction between the active aldehyde of OCMC and the amino groups of the carboxymethyl chitosan (CMCS). The structure of the hydrogels was characterized by FTIR and scanning electron microscopy. Gelation time test showed that the hydrogel had the shortest gelation time of 24 s. The equilibrium fluid content, which represented the swelling degree, was evaluated and we found that the pH increased from 3.0 to 9.0, the equilibrium fluid content increased, and the highest equilibrium fluid content reached 312.83% as pH ¼ 9.0. The wound healing efficacy of the hydrogel was evaluated in experimental deep second degree burns using a rat model. Results indicated that the wound covered with hydrogel was completely filled with new epithelium within 2 weeks, without any significant adverse reactions. The in situ crosslinked hydrogel fulfilled many critical elements in a wound dressing material.

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“…[7] Hydrogels may require further chemical processing to introduce the important cellular chemical cues. [8] This introduces the potential for residual chemicals to be entrapped within the gel, influencing the reproducibility of cell development. These materials additionally have the potential to degrade after prolonged exposure to culture media.…”

Section: D Carbon Scaffolds For Neural Stem Cell Culture and Magnetimentioning

confidence: 99%

3D Carbon Scaffolds for Neural Stem Cell Culture and Magnetic Resonance Imaging

Fuhrer

Bäcker

Kraft

et al. 2017

Adv Healthcare Materials

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3D Carbon Scaffolds for Neural Stem Cell Culture and Magnetic Resonance ImagingErwin Fuhrer, Anne Bäcker, Stephanie Kraft, Friederike J. Gruhl, Matthias Kirsch, Neil MacKinnon, Jan G. Korvink, and Swati Sharma* DOI: 10.1002/adhm.201700915 the dimensionality of the culture system. Many in vitro assays are 2D substrate systems, which raises the question of the relevance to tissues that thrive in a 3D environment. Since it has been recognized that the scaffold topology imparts important cues for cell development and function, there has been a concerted effort to develop 3D scaffold systems that better reproduce the in vivo environment.Common materials used for 3D scaffold fabrication for various cell differentiation, angiogenesis, and tumor growth studies include commercially available Matrigel, hydrogels, and cryogels. [2] Matrigel is a gelatinous protein mixture that contains components of the basement membrane, such as laminin, collagen type IV, and entactin. [3] Hydrogels (water-containing polymer networks) are composed of either natural polymers such as collagen, hyaluronates, fibrin, and chitosan or synthetic polymers such as polyvinyl caprolactam and polyethylene glycol. [4] These materials are generally advantageous since a 3D hydrated network can be produced together with the desired chemical cues required for cell development (e.g., growth factors and cell adhesion promoters). In addition, the porous structure (size and shape) of the polymer network can be tuned by varying the freezing conditions and concentration of the cross-linker in the case of cryogels. [5,6] However, several disadvantages can be identified. Matrigel is often poorly chemically defined and thus culture-to-culture variation has been observed. [7] Hydrogels may require further chemical processing to introduce the important cellular chemical cues. [8] This introduces the potential for residual chemicals to be entrapped within the gel, influencing the reproducibility of cell development. These materials additionally have the potential to degrade after prolonged exposure to culture media. While not always an issue, in cases of extended culture times, cell cultures require a scaffold that will maintain its mechanical integrity. In this work, we propose glassy carbon as a material capable of overcoming these challenges.Conversion of polymer structures into glassy carbon using pyrolysis is a widespread process that is extensively used for the fabrication of carbon MEMS and NEMS, [9,10] battery and supercapacitor anodes, [11] and carbon nanofibers. [12,13] One very attractive yet rather unexplored feature of glassy carbon is its excellent cytocompatibility, [14][15][16][17][18] which, in combination with its mechanical strength, [9] inertness, and patternability, [10] makes it a very suitable material for the fabrication of 3D cell culture platforms.

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Antimicrobial Susceptibility, Heavy Metals Tolerance and Plasmid Curing of <i>Shigella</i> Species Isolated from El- Dakahlia, Egypt

Cited by 12 publications

References 34 publications

Characterization of thin gelatin hydrogel membranes with balloon properties for dynamic tissue engineering

Characterization of thin gelatin hydrogel membranes with balloon properties for dynamic tissue engineering

Preparation, characterization and the effect of carboxymethylated chitosan–cellulose derivatives hydrogels on wound healing

3D Carbon Scaffolds for Neural Stem Cell Culture and Magnetic Resonance Imaging

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