Antimicrobial Susceptibility and Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Tunisia: Results of a Multicenter Study

Mezghani, M; Jdidi, Trabelsi Jihene; Gustave, Claude-Alexandre; Boutiba, Ilhem; Mastouri, Maha; Sophia, Besbes; Barguellil, Farouk; Frédéric, Laurent; Hammami, Adnène

doi:10.23937/2474-3658/1510071

Cited by 6 publications

(5 citation statements)

References 65 publications

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“…In our study, all the MRSA isolates were susceptible to linezolid and tigecycline. The MIC 50 , MIC 90 and the mean MIC were similar to those reported in the previously study from Tunisia [21].…”

Section: Figure1 Population Analysis Profile Curves Of Four Isolatessupporting

confidence: 87%

“…Our study documented the presence of 0.6% hVISA and 2.5% teicoplanin resistance amongst MRSA isolates. The previously study in Tunisia reported hVISA and teicoplanin resistance prevalence of 0.8% [21]. No resistance to vancomycin was noted in our study and in Tunisia [21].…”

Section: Figure1 Population Analysis Profile Curves Of Four Isolatessupporting

confidence: 50%

“…The previously study in Tunisia reported hVISA and teicoplanin resistance prevalence of 0.8% [21]. No resistance to vancomycin was noted in our study and in Tunisia [21]. VRSA due to the acquisition of the vanA gene from enterococci are currently very low.…”

Section: Figure1 Population Analysis Profile Curves Of Four Isolatescontrasting

confidence: 41%

“…The pooled means of vancomycin MIC were 1.12 µg/ml in Europe, 1.17% in Asia and 1.37% in USA [20]. In Tunisia, only one multicenter study, conducted between 2011 and 2012, evaluated the activity of glycopeptides on MRSA by determination of MICs by BMD [21].By comparing the results of this multicenter study with the present study, we observed an increase in the geometric means for vancomycin MIC (0.73to 0.90 µg/ml) and for teicoplanin MIC (0.49 to 0.92 µg/ml), accompanied by an increase in the percentage of strains with vancomycin MIC > 1µg/ml (from 1.5% to 3.7%).…”

Section: Figure1 Population Analysis Profile Curves Of Four Isolatesmentioning

confidence: 99%

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In Vitro Susceptibility to Glycopeptides, Linezolid, Tigecycline, and Ceftaroline against Methicillin Resistant Staphylococcusaureus Isolates in a Tunisian University Hospital

Mezghani Maalej,

Gargouri,

Hammami

2020

Ann Microbiol Infect Dis

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Background: Vancomycin remains the drug of choice for treatment of serious methicillin-resistant Staphylococcus aureus (MRSA) infections. However, Strains with reduced susceptibility to vancomycin have been reported in several countries around the world.Therefore, new antibiotics are introduced into the treatment. Objectives: The present study aimed to determine the vancomycin, teicoplanin, linezolid, tigecycline and ceftaroline susceptibility patternand to investigate the presence and the frequency of heterogeneous vancomycin intermediate S. aureus (hVISA) among clinical isolates of MRSA in Tunisia. Methods: A total of 162 non duplicate MRSA strains isolated between 2017 and 2018 were investigated. Vancomycin, teicoplanin, tigecycline, linezolid and ceftaroline minimum inhibitory concentrations (MIC) values were detected by broth microdilution method and interpreted according to the European Committee on Antimicrobial Susceptibility testing criteria. Etest GRD, Etestmacromethod, Mueller-Hinton screen agar, and population analysis profile-area under the curve (PAP-AUC) methods were used to detect hVISA. Results: The MIC 50 , MIC 90 , and MIC ranges were respectively 1, 1, and 0.5-2 µg/ml for vancomycin; 1, 2, and 0.125-4 µg/ml for teicoplanin; 2, 2, and 0.5-4 µg/ml for linezolid; and 0.25, 0.5, and 0.064-0.5 µg/ml for tigecycline. Twelve strains were suspected as hVISA by EtestGRD, 28 by screen agar, and one by Etestmacromethod, but only one strain was confirmed hVISA by PAP-AUC. No vancomycin, tigecycline and linezolid resistance was found among MRSA isolates. Four strains were teicoplanin resistant, and four were intermediate to ceftaroline. Conclusion:The prevalence of hVISA, teicoplanin and ceftaroline resistance are low. Linezolid and tigecycline were found to be highly active against MRSA isolates. Therefore, they could be considered as alternative agents for the treatment of serious infections. Continuous and regular monitoring of MICs at local and regional level is necessary to guide clinician in their empiric antibiotic selection.

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Section: Figure1 Population Analysis Profile Curves Of Four Isolatessupporting

confidence: 87%

Section: Figure1 Population Analysis Profile Curves Of Four Isolatessupporting

confidence: 50%

Section: Figure1 Population Analysis Profile Curves Of Four Isolatescontrasting

confidence: 41%

Section: Figure1 Population Analysis Profile Curves Of Four Isolatesmentioning

confidence: 99%

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In Vitro Susceptibility to Glycopeptides, Linezolid, Tigecycline, and Ceftaroline against Methicillin Resistant Staphylococcusaureus Isolates in a Tunisian University Hospital

Mezghani Maalej,

Gargouri,

Hammami

2020

Ann Microbiol Infect Dis

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“…Moreover, strains clustered within a single pulso-type also showed similar antimicrobial resistance properties. Other studies have also highlighted correlations between strain relatedness and antimicrobial resistance patterns [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Clonal diversity of Staphylococcus aureus isolates in clinical specimens from selected health facilities in Ethiopia

Ibrahim,

Mekuria,

Wang

et al. 2023

BMC Infect Dis

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Staphylococcus aureus is among the top three causative agents of nosocomial infection in Ethiopia. The majority of studies in Ethiopia have focused on the epidemiology of S. aureus in hospital settings, with limited molecular genotyping results. Molecular characterization of S. aureus is essential for identification of strains, and contributes to the control and prevention of S. aureus infection. The aim of the current study was to determine the molecular epidemiology of methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolates recovered from clinical specimens in Ethiopia. A total of 161 MSSA and 9 MRSA isolates were characterized using pulsed-field gel electrophoresis (PFGE) and staphylococcal protein A (spa) typing. Based on the PFGE analysis, MSSA isolates were grouped into eight pulso-types groups (from A to I), while MRSA isolates clustered into three (A, B and C) pulso-types with more than 80% similarity. The spa typing analysis showed diversity of S. aureus with 56 distinct spa types. Spa type t355 was most prevalent (56/170, 32.9%), while eleven new spa types were detected including t20038, t20039, and t20042. The identified spa types were clustered into 15 spa-clonal complexes (spa-CCs) using BURP analysis; novel/unknown spa types were further subjected to MLST analysis. The majority of isolates belonged to spa-CC 152 (62/170, 36.4%), followed by spa-CC 121 (19/170, 11.2%), and spa-CC 005 (18 /170, 10.6%). Of the nine MRSA isolates, 2 (22.2%) were spa-CC 239 with staphylococcal cassette chromosome (SCC)mec III. These findings highlight the diversity of S. aureus strains in Ethiopia, as well as the presence of potentially epidemic strains circulating in the country necessitating further characterization of S. aureus for antimicrobial resistance detection and infection prevention purposes.

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A review of horses as a source of spreading livestock-associated methicillin-resistant Staphylococcus aureus to human health

et al. 2022

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) was first discovered in horses in 1989. Since then, LA-MRSA has begun to be considered an important strain of pathogenic bacteria in horses, which can cause LA-MRSA infection and colonization in humans with public health impacts. The anterior nares are the primary site of LA-MRSA colonization in horses, although LA-MRSA colonization may also occur in the gastrointestinal tract in horses. LA-MRSA-infected horses typically exhibit clinical infection or may not exhibit clinical infection. There are two potential risks associated with LA-MRSA colonization in horses: The possibility of disease development in horses infected with LA-MRSA and the possibility of LA-MRSA transfer to humans and other horses. The diagnosis of LA-MRSA in horses can be made by conducting in vitro sensitivity testing for oxacillin and cefoxitin, and then followed by a molecular test using polymerase chain reaction. LA-MRSA transmission in animal hospitals and on farms is most likely due to contact with horses infected or colonized by LA-MRSA. The history of prior antibiotic administration, history of prior LA-MRSA colonization, and length of equine hospitalization were described as risk factors in cases of infection and colonization of LA-MRSA in horses. Nebulized antibiotics may be a viable alternative to use in horses, but nebulized antibiotics are only used in horses that are persistently colonized with LA-MRSA. Controlling the spread of LA-MRSA in horses can be done by regularly washing horses, eradicating vectors in horse stalls such as rats, and maintaining the cleanliness of the stable and animal hospital environment. Meanwhile, cleaning hands, using gloves, and donning protective clothes are ways that humans can prevent the transmission of LA-MRSA when handling horses. This review will explain the definition of LA-MRSA in general, LA-MRSA in horses, the epidemiology of LA-MRSA in horses, the diagnosis of LA-MRSA in horses, the transmission of LA-MRSA in horses, risk factors for spreading LA-MRSA in horses, public health impact, treatment of LA-MRSA infection in horses, and control of the spread of LA-MRSA in horses.

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Antimicrobial Susceptibility and Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Tunisia: Results of a Multicenter Study

Cited by 6 publications

References 65 publications

In Vitro Susceptibility to Glycopeptides, Linezolid, Tigecycline, and Ceftaroline against Methicillin Resistant Staphylococcusaureus Isolates in a Tunisian University Hospital

In Vitro Susceptibility to Glycopeptides, Linezolid, Tigecycline, and Ceftaroline against Methicillin Resistant Staphylococcusaureus Isolates in a Tunisian University Hospital

Clonal diversity of Staphylococcus aureus isolates in clinical specimens from selected health facilities in Ethiopia

A review of horses as a source of spreading livestock-associated methicillin-resistant Staphylococcus aureus to human health

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