Antimicrobial susceptibility and immunomodulatory properties of lamb isolate of lactobacillus mucosae, new probiotic candidate

Abstract

In the process of selecting a new probiotic candidate, several bacteria were isolated from the stomach mucosa of a lamb. Among them, three lactobacilli strains were identified and partially characterised. The strain, Lactobacillus mucosae D, showed several characteristics appropriate to the probiotics. In this study, we have focused on the further characterisation of L. mucosae D and testing of its ability to modulate metabolic and immunomodulatory activities of human mononuclear cells in vitro. L. mucosae D is resistant to antibiotics, like penicillin G, oxacillin, vancomycin and chemotherapeutics ofloxacin and ciprofloxacin. In in vitro conditions, L. mucosae D caused a significant increase in phagocytic activity and index (relative activities 1.05 and 1.44, respectively) of human monocytes. It decreased bactericidal activities of monocytes against Escherichia coli (relative activity 0.73) and Staphylococcus aureus (relative activity 0.36), whereas, candidacidal activity was enhanced (relative activity 1.15). Metabolic activities, lysozyme and peroxidase activity, of mononuclear cells were not changed or increased, respectively. L. mucosae D displayed the ability to enhance production of pro-inflammatory cytokine, IL-1β, in monocytes in vitro (relative activity 2.60). Therefore, we state that lamb isolate, L. mucosae D, has the required attributes for being a potential probiotic candidate.

Isolation and characterization of a novel exopolysaccharide secreted by Lactobacillus mucosae VG1

“…Strains of Lactobacillus mucosae (L. mucosae) are interesting potential candidates for use as probiotics [4][5][6][7][8][9][10][11] as they have the capacity to colonise host mucosal niches [12] and specific strains have been shown to be both bile and acid tolerant [5,13], which would facilitate their survival in the GIT. L. mucosae were first identified as a new species in 2000 by Roos et al [6] who used a genetic probe to detect bacterial strains with mucus-binding activity (using the mucin binding-gene probe mub).…”