Antimicrobial susceptibility and genetic characteristics of Haemophilus influenzae isolated from community-acquired respiratory tract infection patients in Shanghai City, China

Qin, Liang; ZhaoYan, Zhou; Hu, Bijie; Yamamoto, Taro; Watanabe, Hiroshi

doi:10.1007/s10156-012-0372-0

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…Qin et al showed that most H. influenzae isolates (30/37, 81.1%) causing community-acquired pneumonia (CAP) in adults were NTHi in Shanghai in 2009. In addition, the rates of strains with β-lactamase and of BLNAR accounted for 27.0 and 29.8%, respectively (23). In our study, we found all isolates from adult patients were nontypeable, the 33.3% of the isolates enrolled produced β-lactamase and 11.76% possessing mutation in PBP3 were resistant to ampicillin, which indicated that the production of β-lactamase was increased and BLNAR was more frequent in adult patients than pediatric patients.…”

Section: Discussionmentioning

confidence: 47%

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Xiao

et al. 2020

Front. Public Health

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Background: The serotype and antimicrobial resistance of Haemophilus influenzae in adult patients have changed due to the application of antimicrobials and H. influenzae type b (Hib) vaccine worldwide. However, the epidemiologic characteristics of H. influenzae in Shanghai are still unavailable. Objective: To determine the serotype distribution, antimicrobial resistance and multilocus sequence type (MLST) of H. influenzae in adult patients in Shanghai. Methods: A total of 51 clinical isolates from adult patients were consecutively collected. Serotypes were determined according to specific capsule gene, bexA, amplified by PCR. Antimicrobial susceptibility test was carried out by the broth microdilution method. β-lactamase production was detected by cefinase disk and the ftsI gene were amplified and sequenced to determine the penicillin binding protein 3 (PBP3) mutation. Molecular epidemiology was performed by MLST analyses. Results: All isolates studied were nontypeable H. influenzae (NTHi) and three of them (5.88%) caused invasive infection. The resistant rates of ampicillin and trimethoprim/sulfamethoxazole were both 45.10%. One third of these isolates produced TEM-1 type β-lactamase and 11.76% were β-lactamase negative ampicillin resistant strains (BLNAR). The PBP3 mutation was detected in 74.51% of the isolates, of which 12 belonged to group III. A total of 36 sequence types (STs) were identified among all isolates. Four isolates of ST103 (7.84%) all produced β-lactamase without mutation of PBP3. Conclusion: H. influenzae infections among adults in Shanghai are predominately caused by NTHi with genetic diversity among adult patients. The prevalence of both β-lactamase production and PBP3 mutation may contribute to high ampicillin resistance rate in Shanghai.

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Section: Discussionmentioning

confidence: 47%

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Xiao

et al. 2020

Front. Public Health

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“…In the past, low-BLNAR isolates were clinically predominant (10,11,15,18,19). However, high-BLNAR isolates have emerged since 1998 (19).…”

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confidence: 99%

“…In the past, low-BLNAR isolates were clinically predominant (10,11,15,18,19). However, high-BLNAR isolates have emerged since 1998 (19). Whereas low-BLNAR isolates constitute the majority of BLNAR strains in the United States and many European countries (7, 9-11, 14, 15), high-BLNAR isolates have significantly increased in prevalence in Japan and South Korea since 2005 (6,8,13,(20)(21)(22)(23).…”

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confidence: 99%

Multiclonal Expansion and High Prevalence of β-Lactamase-Negative Haemophilus influenzae with High-Level Ampicillin Resistance in Japan and Susceptibility to Quinolones

Honda

Sato

Shinagawa

et al. 2018

Antimicrob Agents Chemother

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β-Lactam-resistant is a clinical concern. A high prevalence (>40%) of β-lactamase-negative high-level ampicillin-resistant (high-BLNAR) isolates in Japan has been reported. However, the reasons for the expansion are unknown. High-BLNAR strains possess an amino acid substitution, either Asn526Lys (group III) or Arg517His (group III-like) in addition to Ser385Thr, in penicillin-binding protein 3 (PBP3). To determine the current prevalence of high-BLNAR strains and the mechanisms behind their expansion in Japan, their prevalence, PBP3 types, multilocus sequence types, and susceptibilities to quinolones approved in Japan as alternatives were determined. Sixty percent of clinical isolates (62/104 isolates) were β-lactamase-negative ampicillin-resistant (BLNAR) strains. Among BLNAR isolates, 92% (57/62 isolates) were high-BLNAR strains. Most isolates were classified as belonging to group III, which contained many genotypes (11 PBP3 types and 25 sequence types). These results indicated that the expansion of high-BLNAR isolates was multiclonal and such strains are still predominant in Japanese clinical settings. One high-BLNAR isolate harbored the novel amino acid substitution Asn526Met in addition to Ser385Thr in PBP3, suggesting a new group (group IV). No quinolone-resistant isolates were identified. The MICs for the quinolones (moxifloxacin, garenoxacin, and tosufloxacin) were similar to that for levofloxacin, whereas sitafloxacin exhibited a lower MIC. However, we obtained 4 isolates with decreased quinolone susceptibility with the amino acid substitution Ser84Leu in GyrA, and 3 of those isolates were high-BLNAR isolates. In summary, this study shows that multiclonal high-BLNAR strains predominate in a Japanese university hospital. Isolates remain sensitive to quinolones, but vigilance is required to prevent the development of fluoroquinolone resistance in high-BLNAR strains.

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“…On the other hand, strain P3271/A isolated from the blood showed a positive serology result for type b and positive PCR result for capsule determination and capsular type, which confirms that this isolate was a regular Hib strain. MLST analysis was performed as previously described by Meats et al (2003) and typing based on the bacterial genomic DNA fingerprinting pattern obtained by PFGE has been reported to determine whether the two isolates were the same strain (Smith & Cantor, 1987;Tenover et al, 1995;Saito et al, 1999;Qin et al, 2012). Both strains were classified as ST-6 by MLST analysis and PFGE analysis confirmes the MLST result, since the two strains showed identical fingerprinting patterns (Fig.…”

mentioning

confidence: 82%

Haemophilus influenzae serotype b and a capsule-deficient type mutant (b−) invasive disease in a partially vaccinated child in Brazil

Caldeira

Filippis

Arruda

et al. 2013

Journal of Medical Microbiology

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We report a rare case of infection by two different types of Haemophilus influenzae strains in a child who received only one dose of the H. influenzae serotype b (Hib) conjugate vaccine (DTwP+Hib). The strains were recovered from blood and cerebrospinal fluid (CSF) and were phenotypically identified as Hib and non-typable H. influenzae, respectively, after serological tests. The two strains were characterized by PCR capsular typing, multilocus sequence typing and PFGE. Our results suggest that the infection was caused by the bloodstream invasion by a single Hib strain, followed by the diffusion of the bacteria across the blood-brain barrier and into the CSF. The strain recovered from the CSF, however, was identified as a capsule-deficient type mutant (b " ) strain. Despite the high efficacy of the Hib conjugate vaccine, the increase in the numbers of strains able to escape the immune system of the vaccinated population advocates continued surveillance.

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Antimicrobial susceptibility and genetic characteristics of Haemophilus influenzae isolated from community-acquired respiratory tract infection patients in Shanghai City, China

Cited by 7 publications

References 30 publications

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Multiclonal Expansion and High Prevalence of β-Lactamase-Negative Haemophilus influenzae with High-Level Ampicillin Resistance in Japan and Susceptibility to Quinolones

Haemophilus influenzae serotype b and a capsule-deficient type mutant (b−) invasive disease in a partially vaccinated child in Brazil

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