Antimicrobial Susceptibility and Enterotoxin-Encoding Genes inStaphylococcusspp. Recovered from Kitchen Equipment from a University Hospital in Rio de Janeiro, Brazil

Abstract

This study was conducted to determine the occurrence of antimicrobial resistance and enterotoxin-encoding genes (EEGs) in Staphylococcus spp. recovered from equipment used to prepare hospital meals, in a university hospital in Rio de Janeiro, Brazil. Sixty samples were collected from semi-industrial equipment (one blender and one mixer) in the hospital's kitchen. Resistance genes and SCCmec types were detected by PCR. From the 40 isolates of Staphylococcus spp. identified, 8 were Staphylococcus aureus. Thirty-two (80%) Staphylococcus spp. isolates were resistant to at least one antimicrobial agent. Resistance genetic determinants were detected: erm gene (Staphylococcus epidermidis [n = 2]; Staphylococcus hominis [n = 1]), mecA gene (S. epidermidis [n = 2]), and aa(6')-aph(2'') gene (Staphylococcus caprae [n = 1], S. epidermidis [n = 2], S. hominis [n = 1], Staphylococcus pausteri [n = 1], Staphylococcus simulans [n = 1], and Staphylococcus warneri [n = 1]). The presence of at least one EEG in 83% (n = 33) of the isolates was identified. Two strains of S. epidermidis were methicillin-resistant S. epidermidis (MRSE) and harboring SCCmec type IV. Staphylococcus spp. contaminated some hospital kitchen's equipment, indicating that hygiene procedures should be improved. Results also indicate that meals can be a vehicle to disseminate multiresistant Staphylococcus spp., including MRSE, and Staphylococcus with EEGs.

Hygiene management for long-term ventilated persons in the home health care setting: a scoping review

“…This is potentially hazardous to immunocompromised patients. Even in the hospital environment, the occurrence of Staphylococcus bacteria in kitchen equipment was shown [ 46 ]. Taché et al [ 43 ] stated in their review on hygiene in the home kitchen that settings not under the control of competent authority are at higher risk for food-borne infectious diseases.…”

Avaliação da qualidade microbiológica de fórmulas infantis desidratadas após reconstituição e durante o armazenamento no lactário de um Hospital Universitário

“…No que diz respeito à contaminação advinda do uso de equipamentos, Miyahira et al 18 , em pesquisa conduzida em um Hospital Universitário do Rio de Janeiro, constataram que o liquidificador e a batedeira utilizados no preparo de alimentos eram veículos de contaminação por Staphylococcus spp. Tal resultado reforça a importância da correta higienização dos utensílios e equipamentos antes dos processos produtivos.…”

Invasive Diseases Due to Fatal Staphylococcus caprae Infection: A Case Series and Literature Review

“…Staphylococcus caprae belongs to the broad family of coagulase-negative Staphylococci (conS), and is a potential colonizer of human skin. Initially isolated from goat milk, its has been sufficiently characterized from a bacteriological point of view since the year 1995 [1][2][3][4][5][6][7][8][9][10][11], although some unexpected features like the possible production of S. aureus-like enterotoxins deserve further studies [7,12,13].…”