Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use

Klare, Ingo; Konstabel, Carola; Werner, Guido; Huys, Geert; Vankerckhoven, Vanessa; Kahlmeter, Gunnar; Hildebrandt, Bianca; Müller-Bertling, Sibylle; Witte, Wolfgang; Goossens, Herman

doi:10.1093/jac/dkm035

Cited by 310 publications

(245 citation statements)

References 54 publications

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“…However, the resistance towards vancomycin has been demonstrated being as intrinsic (Tynkkynen et al, 1998). Lactobacilli are usually susceptible to chloramphenicol, erythromycin and clindamycin (protein synthesis inhibitors) (Coppola et al, 2005;Klare et al, 2007). In addition, resistance against trimethoprim (nucleic acid synthesis inhibitor), seems to be intrinsic (Ammor et al, 2007).…”

Section: Antibiotic Resistance Of Probiotic Lactobacillimentioning

confidence: 99%

Assessment of Antibiotic Resistance in Probiotic Lactobacilli

Fukao¹,

Yajima²

2012

Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium

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Section: Antibiotic Resistance Of Probiotic Lactobacillimentioning

confidence: 99%

Assessment of Antibiotic Resistance in Probiotic Lactobacilli

Fukao¹,

Yajima²

2012

Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium

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“…Characteristics such as total security for the host, resistance to gastric acidity and pancreatic secretions, adhesion to intestinal epithelial cells, antimicrobial activity, inhibition of the adhesion of pathogenic bacteria, antibiotic resistance, tolerance to food additives and stability in the food matrix, are all considered relevant in tracing the probiotic profile of a specific strain (Soccol et al 2010). Pediococci are used as beneficial microorganisms in the context of food and livestock microbiology (Haakensen et al 2009) and can be used as starter cultures or as probiotics (Klare et al 2007). P. acidilactici has interesting properties, such as resistance to heat, cold, pH, proteolytic treatments, and the capacity for the production of antimicrobial peptide (pediocin) with anti-listerial activity and antimicrobial spectrum, which makes it interesting as a food biopreservative (Papagianni and Anastasiadou 2009).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of probiotic properties of Pediococcus acidilactici B14 in association with Lactobacillus acidophilus ATCC 4356 for application in a soy based aerated symbiotic dessert

Ribeiro

Vandenberghe

Spier

et al. 2014

Braz. arch. biol. technol.

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“…Thumu & Halami (2012) and Nawaz et al (2011) reported that many LAB resistant to erythromycin, tetracycline, and chloramphenicol related to the presence of gene encoding resistant due to mutation or mobile genes. In contrast, Klare et al (2007) reported that gram-positive bacteria are generally sensitive to antibiotics that inhibit the protein synthesis such as erythromycin and chloramphenicol. Isolates of E5, E7, and E8 did not resistant to erythromycin, chloramphenicol, and tetracycline.…”

Section: Susceptibility To Antibioticsmentioning

confidence: 99%

Selection of Lactic Acid Bacteria as Probiotic Candidate for Chicken

Hamida

Wiryawan

Meryandini

2015

Med Pet

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Lactic acid bacteria (LAB) regarded as safe microorganisms; they can naturally live in gastrointestinal tract, so appropriately used as a probiotic for chicken. This study aimed to select six isolates of LAB (E1223, E3, E4, E5, E7, and E8) to obtain the isolates potentially as probiotic candidate for chicken. The six isolates were derived from spontaneous fermented corn obtained from Laboratory of Animal Biotechnology and Biomedical, PPSHB, Bogor Agricultural University, Indonesia. LAB isolates were tested their susceptibility to antibiotics (bambermycin, erythromycin, chloramphenicol, and tetracycline) then were examined in vitro for their tolerance to gastrointestinal pH (2, 3, 4, and 7.2) and 0.5% bile salt condition, antimicrobial activity against Salmonella enteritidis and Enterococcus casseliflavus, and ability to adhere to chicken ileal cells. The results showed the isolates E5, E7, and E8 were sensitive to tetracycline and chloramphenicol, they could survive at pH 2, 3, 4, and 7.2, could survive at 0.5% bile salts, produced antimicrobial activity, and able to adhere to ileal cells (9.40±0.00 Log CFU/cm 2 of E8) and were significantly (P<0.05) higher than those of control (5.30±0.14 Log CFU/cm 2 ). In conclusion, this study showed that isolate E8 had better potential compared to isolates E5 and E7 in most in vitro assays as a probiotic candidate for chicken. E5, E7, and E8 were closely related with Pediococcus pentosaceus based on 16S rRNA gene. Key words: LAB, probiotic, chicken, in vitro ABSTRAKBakteri asam laktat (BAL) dianggap sebagai mikroorganisme aman, dapat hidup di dalam saluran pencernaan, sehingga tepat digunakan sebagai mikroorganisme probiotik untuk ayam. Penelitian ini bertujuan menyeleksi secara in vitro enam isolat BAL (E1223, E3, E4, E5, E7, E8) untuk memperoleh BAL berpotensi sebagai kandidat probiotik ayam. Enam isolat berasal dari jagung fermentasi yang diperoleh dari Laboratorium Bioteknologi Hewan dan Biomedis, PPSHB, Institut Pertanian Bogor, Indonesia. Isolat BAL diuji sensitifitasnya terhadap antibiotik (bambermisin, eritromisin, kloramfenikol, dan tetrasiklin) lalu diuji secara in vitro ketahanannya terhadap pH saluran pencernaan (2; 3; 4; dan 7,2) dan 0,5% garam empedu, aktivitas antimikroba terhadap Salmonella enteritidis dan Enterococcus casseliflavus, dan kemampuan menempel pada ileum ayam. Hasil penelitian menunjukkan bahwa isolat E5, E7, dan E8 sensitif terhadap tetrasiklin dan kloramfenikol, toleran pada pH pencernaan dan garam empedu, menghasilkan aktivitas antimikrob, dan mampu menempel pada ileum ayam (sel isolat E8 yang menempel sebesar 9,40±0,00 Log CFU/cm 2 ) signifikan (P<0,05) lebih tinggi dibandingkan dengan kontrol (5,30±0,14 Log CFU/cm 2 ). Kesimpulan penelitian ini menunjukkan bahwa isolat E8 memiliki potensi lebih baik daripada isolat E5 dan E7 di sebagian besar uji in vitro sebagai kandidat probiotik ayam. Isolat E5, E7, dan E8 memiliki homologi 99% dengan Pediococcus pentosaceus berdasarkan gen 16S rRNA.

show abstract

Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use

Abstract: Finding of acquired resistance genes in isolates intended for probiotic or nutritional use highlights the importance of antimicrobial susceptibility testing in documenting the safety of commercial LAB.

Cited by 310 publications

References 54 publications

Assessment of Antibiotic Resistance in Probiotic Lactobacilli

Assessment of Antibiotic Resistance in Probiotic Lactobacilli

Evaluation of probiotic properties of Pediococcus acidilactici B14 in association with Lactobacillus acidophilus ATCC 4356 for application in a soy based aerated symbiotic dessert

Selection of Lactic Acid Bacteria as Probiotic Candidate for Chicken

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