Antimicrobial Silver Chloride Nanoparticles Stabilized with Chitosan Oligomer for the Healing of Burns

Kang, Yun Ok; Jung, Jongwan; Cho, Donghwan; Kwon, Oh Hyeong; Cheon, Ja Young; Park, Won Ho

doi:10.3390/ma9040215

Cited by 50 publications

(28 citation statements)

References 31 publications

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“…The crystalline nature of Ag/AgCl‐NPs and the presence of silver and silver chloride were confirmed by XRD. The formation of AgCl‐NPs was explained by Kang et al who revealed that synthesis of AgCl‐NPs from oligomeric chitosan occurred in two steps 26 . At first, Ag ions readily reacted with Cl ions and the amino and hydroxyl groups stabilized the AgCl‐NPs.…”

Section: Discussionmentioning

confidence: 99%

Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo

Kabir

Dai

Nurujjaman

et al. 2020

J Cellular Molecular Medi

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The importance of biogenic silver/silver chloride nanoparticles has become increasing day by day. In the present study, silver/silver chloride nanoparticles (Ag/AgCl‐NPs) were synthesized from Kaempferia rotunda tuberous rhizome extract to evaluate the antiproliferative activity against human glioblastoma stem cells (GSCs) in vitro and Ehrlich ascites carcinoma (EAC) cells in vivo in mice. Synthesis of nanoparticles was confirmed by colour change and UV‐visible spectrum and characterized by TEM, XRD, TGA, AFM and FTIR. K rotunda and recently synthesized Zizyphus mauritiana fruit extract‐mediated Ag/AgCl‐NPs inhibited 77.2% and 71% of GSCs growth at 32 µg/mL concentration with the IC50 values of 6.8 and 10.4 µg/mL, respectively. Cell morphological studies and caspase‐3 immunofluorescence assay revealed that both biogenic nanoparticles induced apoptosis in GSCs. Expression levels of several genes were checked by real‐time PCR after treatment with K rotunda tuberous rhizome‐mediated Ag/AgCl‐NPs. PARP, EGFR, NOTCH2 and STAT3 gene expression were decreased with the increase of NFκB, TLR9, IL1, TNFα, IKK and p21 gene that would be the cause of induction of apoptosis in GSCs. The cell cycle arrest at G2/M phase was confirmed by flow cytometric assay. Both nanoparticles were injected intraperitoneally to rapidly growing EAC cells for 5 consecutive days. Approximately, 32.3% and 55% EAC cells growth were inhibited by K rotunda tuberous rhizome‐mediated Ag/AgCl‐NPs at 6 and 12 mg/kg/day doses, respectively while only 20% cell growth inhibition was monitored at 12 mg/kg/day dose of Z mauritiana‐mediated Ag/AgCl‐NPs. From the above results, it can be concluded that presently synthesized nanoparticles would be a potent anticancer agent.

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Section: Discussionmentioning

confidence: 99%

Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo

Kabir

Dai

Nurujjaman

et al. 2020

J Cellular Molecular Medi

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“…It is also possible that Ag/ AgCl-NPs interact with the actin cytoskeleton, causing structural and functional damage to cytoskeletal elements (Urbańska et al 2015). In addition, the active surface of AgNPs can directly induce the generation of free radicals and the dissolution of AgNPs into Ag ions, which is a characteristic shared by silver-based nanoparticles, including Ag/AgCl-NPs (Kang et al 2016). Ag ions damage the DNA and disrupt of the mitochondrial membrane potential, leading to the release of cytochrome c and to mitochondrial-dependent apoptosis (Hsin et al 2008;Piao et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Silver/silver chloride nanoparticles inhibit the proliferation of human glioblastoma cells

et al. 2018

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Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of biogenically produced Ag/AgCl-NPs (from yeast cultures) on the proliferation of GBM02 glioblastoma cells (and of human astrocytes) by automated, image-based high-content analysis (HCA). We compared the effect of 0.1-5.0 µg mL Ag/AgCl-NPs with that of 9.7-48.5 µg mL temozolomide (TMZ, chemotherapy drug currently used to treat glioblastomas), alone or in combination. At higher concentrations, Ag/AgCl-NPs inhibited GBM02 proliferation more effectively than TMZ (up to 82 and 62% inhibition, respectively), while the opposite occurred at lower concentrations (up to 23 and 53% inhibition, for Ag/AgCl-NPs and TMZ, respectively). The combined treatment (Ag/AgCl-NPs + TMZ) inhibited GBM02 proliferation by 54-83%. Ag/AgCl-NPs had a reduced effect on astrocyte proliferation compared with TMZ, and Ag/AgCl-NPs + TMZ inhibited astrocyte proliferation by 5-42%. The growth rate and population doubling time analyses confirmed that treatment with Ag/AgCl-NPs was more effective against GBM02 cells than TMZ (~ 67-fold), and less aggressive to astrocytes, while Ag/AgCl-NP + TMZ treatment was no more effective against GBM02 cells than Ag/AgCl-NPs monotherapy. Taken together, our data indicate that 2.5 µg mL Ag/AgCl-NPs represents the safest dose tested here, which affects GBM02 proliferation, with limited effect on astrocytes. Our findings show that HCA is a useful approach to evaluate the antiproliferative effect of nanoparticles against tumor cells.

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“…In particular, metal-chitosan (Me-CS) composites have many fascinating applications in catalysis, biosensors and biomaterials [ 24 , 25 ]. A number of studies have been issued on CS combination with different inorganic nanostructures, such as Pd [ 26 , 27 ], Au [ 28 , 29 , 30 , 31 , 32 ], Ag and its compounds [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ], Pt [ 27 , 41 ], Fe and its oxides [ 42 , 43 , 44 ], ZnO [ 20 , 45 , 46 ], zeolite [ 47 ], silica [ 48 ] and graphene [ 49 ], just to cite a few. The inclusion of copper nanophases in CS matrix is quite diffused, as well [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic Characterization of Copper-Chitosan Nanoantimicrobials Prepared by Laser Ablation Synthesis in Aqueous Solutions

et al. 2016

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Copper-chitosan (Cu-CS) nanoantimicrobials are a novel class of bioactive agents, providing enhanced and synergistic efficiency in the prevention of biocontamination in several application fields, from food packaging to biomedical. Femtosecond laser pulses were here exploited to disrupt a Cu solid target immersed into aqueous acidic solutions containing different CS concentrations. After preparation, Cu-CS colloids were obtained by tuning both Cu/CS molar ratios and laser operating conditions. As prepared Cu-CS colloids were characterized by Fourier transform infrared spectroscopy (FTIR), to study copper complexation with the biopolymer. X-ray photoelectron spectroscopy (XPS) was used to elucidate the nanomaterials’ surface chemical composition and chemical speciation of the most representative elements. Transmission electron microscopy was used to characterize nanocolloids morphology. For all samples, ξ-potential measurements showed highly positive potentials, which could be correlated with the XPS information. The spectroscopic and morphological characterization herein presented outlines the characteristics of a technologically-relevant nanomaterial and provides evidence about the optimal synthesis parameters to produce almost monodisperse and properly-capped Cu nanophases, which combine in the same core-shell structure two renowned antibacterial agents.

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Antimicrobial Silver Chloride Nanoparticles Stabilized with Chitosan Oligomer for the Healing of Burns

Cited by 50 publications

References 31 publications

Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo

Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo

Silver/silver chloride nanoparticles inhibit the proliferation of human glioblastoma cells

Spectroscopic Characterization of Copper-Chitosan Nanoantimicrobials Prepared by Laser Ablation Synthesis in Aqueous Solutions

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