Antimicrobial screening of some Egyptian plants and active flavones from Lagerstroemia indica leaves

Diab, Yasser; Atalla, Khaled; Elbanna, Кhaled

doi:10.5582/ddt.2012.v6.4.212

Cited by 10 publications

(9 citation statements)

References 26 publications

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“…This might be the reason that the copy number of E. coli K99 in CT group was significantly lower than that in the P-CON group. A considerable number of studies have demonstrated that flavonoids isolated from the leaves of many different plants show good antimicrobial activity against E. coli 26 , 31 , 33 , 34 . The antimicrobial activity of flavonoids is apparently due to their ability to penetrate biological membranes 35 .…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99

Yang

Diao

et al. 2017

Sci Rep

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The aim of this study was to investigate the effects of dietary supplementation with two alternatives to antibiotics (Candida tropicalis and mulberry leaf flavonoids) on intestinal microbiota of preweaned calves challenged with Escherichia coli K99. Sixty Holstein calves were randomly assigned to 5 treatments: fed a basal diet (N-CON); fed a basal diet and challenged with E.coli K99 (P-CON); fed a basal diet supplemented with C.tropicalis (CT), mulberry leaf flavonoids (MLF), and the combination of the two additives (CM), respectively, and challenged with E.coli K99. The MLF and CM groups had significantly higher average daily grain and feed efficiency, and significantly lower fecal scores compared with the P-CON group after E. coli K99 challenge. The supplementation groups increased the relative abundance, at the phylum level, of Bacteroidetes and Proteobacteria, whereas at the genus level, they increased the relative abundance of Prevotella, Lactobacillus, and Enterococcus. Quantitative PCR revealed that the CT, MLF, and CM groups had significantly lower copy numbers of E.coli K99 compared with the P-CON group. The CT, MLF, and CM treatments reduce days of diarrhea, improve intestinal health, and beneficially manipulate the intestinal microbiota in preweaned calves.

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Section: Discussionmentioning

confidence: 99%

Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99

Yang

Diao

et al. 2017

Sci Rep

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“…E. coli, P. aeruginosa and S. aureus are reference microorganisms to be used in the development of disinfection strategies (EN-1276(EN- 1997. Also, the strains used in this study are commonly used as routine quality control strains, and as reference for antimicrobial testing and for bacterial resistance testing (Ananou et al 2004;Diab et al 2012;Tabata et al 2003;UNE-CEN ISO/ TS 11133 2006). All microbial strains were stored at −80°C in cryovial, 30 % (v/v) glycerol, and subcultured in MuellerHinton Agar (MHA) (Merck, Darmstadt-Germany) at 30°C, before testing.…”

Section: Bacterial Strains and Growth Mediummentioning

confidence: 99%

Antibacterial activity and mode of action of selected glucosinolate hydrolysis products against bacterial pathogens

et al. 2014

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Plants contain numerous components that are important sources of new bioactive molecules with antimicrobial properties. Isothiocyanates (ITCs) are plant secondary metabolites found in cruciferous vegetables that are arising as promising antimicrobial agents in food industry. The aim of this study was to assess the antibacterial activity of two isothiocyanates (ITCs), allylisothiocyanate (AITC) and 2-phenylethylisothiocyanate (PEITC) against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes. The antibacterial mode of action was also characterized by the assessment of different physiological indices: membrane integrity, intracellular potassium release, physicochemical surface properties and surface charge. The minimum inhibitory concentration (MIC) of AITC and PEITC was 100 μg/mL for all bacteria. The minimum bactericidal concentration (MBC) of the ITCs was at least 10 times higher than the MIC. Both AITC and PEITC changed the membrane properties of the bacteria decreasing their surface charge and compromising the integrity of the cytoplasmatic membrane with consequent potassium leakage and propidium iodide uptake. The surface hydrophobicity was also non-specifically altered (E. coli and L. monocytogenes become less hydrophilic; P. aeruginosa and S. aureus become more hydrophilic). This study shows that AITC and PEITC have strong antimicrobial potential against the bacteria tested, through the disruption of the bacterial cell membranes. Moreover, phytochemicals are highlighted as a valuable sustainable source of new bioactive products.

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“…Characterization of surface modifications of nanoparticles was performed by methods that were previously optimized [14,52,[62][63][64][65][66]. The determination of the antioxidant activity of nanoparticles was performed by methods previously optimized using a BS-300 Chemical analyzer from Mindray (Shenzhen, China).…”

Section: Measurement Of Antioxidant Activity Of Silver Nanoparticlesmentioning

confidence: 99%

“…Crape myrtle (Lagerstroemia indica) is an ornamental woody plant used in medicine. Its extract contains many different alkaloids and flavonoids and has anti-inflammatory and antimicrobial effects [49][50][51][52]. Blackboard tree (Alstonia scholaris) has been used in traditional medicine primarily for the treatment of respiratory and inflammatory diseases, malaria, jaundice, and gastrointestinal disorders.…”

Section: Introductionmentioning

confidence: 99%

Effect of Biosynthesized Silver Nanoparticles on Bacterial Biofilm Changes in S. aureus and E. coli

et al. 2022

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One approach for solving the problem of antibiotic resistance and bacterial persistence in biofilms is treatment with metals, including silver in the form of silver nanoparticles (AgNPs). Green synthesis is an environmentally friendly method to synthesize nanoparticles with a broad spectrum of unique properties that depend on the plant extracts used. AgNPs with antibacterial and antibiofilm effects were obtained using green synthesis from plant extracts of Lagerstroemia indica (AgNPs_LI), Alstonia scholaris (AgNPs_AS), and Aglaonema multifolium (AgNPs_AM). Nanoparticles were characterized by transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDX) analysis. The ability to quench free radicals and total phenolic content in solution were also evaluated. The antibacterial activity of AgNPs was studied by growth curves as well as using a diffusion test on agar medium plates to determine minimal inhibitory concentrations (MICs). The effect of AgNPs on bacterial biofilms was evaluated by crystal violet (CV) staining. Average minimum inhibitory concentrations of AgNPs_LI, AgNPs_AS, AgNPs_AM were 15 ± 5, 20 + 5, 20 + 5 μg/mL and 20 ± 5, 15 + 5, 15 + 5 μg/mL against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, respectively. The E. coli strain formed biofilms in the presence of AgNPs, a less dense biofilm than the S. aureus strain. The highest inhibitory and destructive effect on biofilms was exhibited by AgNPs prepared using an extract from L. indica.

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Antimicrobial screening of some Egyptian plants and active flavones from Lagerstroemia indica leaves

Abstract: One hundred and twenty four plant extracts were evaluated for their antimicrobial a c t i v i t y a g a i n s t f o u r p a t h o g e n i c b a c t e r i a (Staphylococcus

Cited by 10 publications

References 26 publications

Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99

Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99

Antibacterial activity and mode of action of selected glucosinolate hydrolysis products against bacterial pathogens

Effect of Biosynthesized Silver Nanoparticles on Bacterial Biofilm Changes in S. aureus and E. coli

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