Antimicrobial resistances of extraintestinal pathogenic Escherichia coli isolates from swine in China

Tang, Xibiao; Tan, Chen; Zhang, Xuan; Zhao, Zhanqin; Xia, Xin; Wu, Bin; Guo, Aizhen; Zhou, Rui; Chen, Huanchun

doi:10.1016/j.micpath.2011.01.004

Cited by 40 publications

(43 citation statements)

References 21 publications

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“…As shown in a previous study, ExPEC strain PCN033 is a multidrug-resistant strain (6). In our sequence, there are at least 50 multidrug resistance-associated genes, including 11 ABC-type multidrug transport system genes, 15 multidrug resistance efflux pump genes, and nine genes similar to arabinose efflux permease genes.…”

supporting

confidence: 63%

“…ExPEC strains are an important group of pathogens which can infect many organs outside the intestine; they mainly include uropathogenic E. coli (UPEC), newborn meningitis-causing E. coli (NMEC), and avian pathogenic E. coli (APEC) (2,3,4). From 2003 onward, many pig ExPEC strains with extensive multidrug resistance were isolated in China (6). These strains cause several diseases, including meningitis, septicemia, and pneumonia, and there have been few studies on pig ExPEC.…”

mentioning

confidence: 99%

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Genome Sequence of a Porcine Extraintestinal Pathogenic Escherichia coli Strain

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supporting

confidence: 63%

mentioning

confidence: 99%

Genome Sequence of a Porcine Extraintestinal Pathogenic Escherichia coli Strain

et al. 2011

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“…Moreover, animals can be reservoirs for these ExPEC strains, which can then be transmitted to humans [4, 5]. For example, in our previous studies, ExPEC isolates detected in 315/3127 (10.1%) pigs among 19 provinces of China exhibited high multidrug resistance (MDR) [6, 7]. One of the main reasons for the threat of ExPEC strains to human health is biofilm formation by ExPEC strains.…”

Section: Introductionmentioning

confidence: 99%

TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity

Hou

Meng

Zhang

et al. 2014

BioMed Research International

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While a high osmolarity medium activates Cpx signaling and causes CpxR to repress csgD expression, and efflux protein TolC protein plays an important role in biofilm formation in Escherichia coli, whether TolC also responds to an osmolarity change to regulate biofilm formation in extraintestinal pathogenic E. coli (ExPEC) remains unknown. In this study, we constructed ΔtolC mutant and complement ExPEC strains to investigate the role of TolC in the retention of biofilm formation and curli production capability under different osmotic conditions. The ΔtolC mutant showed significantly decreased biofilm formation and lost the ability to produce curli fimbriae compared to its parent ExPEC strain PPECC42 when cultured in M9 medium or 1/2 M9 medium of increased osmolarity with NaCl or sucrose at 28°C. However, biofilm formation and curli production levels were restored to wild-type levels in the ΔtolC mutant in 1/2 M9 medium. We propose for the first time that TolC protein is able to form biofilm even under high osmotic stress. Our findings reveal an interplay between the role of TolC in ExPEC biofilm formation and the osmolarity of the surrounding environment, thus providing guidance for the development of a treatment for ExPEC biofilm formation.

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“…Compared with antimicrobial resistance rates of E. coli isolates from other animals (13) and from the environment (30) in China, the rate of resistance to antimicrobials in isolates from giant pandas was much lower. For antibiotic resistance of E. coli isolates from pandas, Zhang et al (31) reported that 32% (19/59) of the fecal bacteria from pandas were resistant to at least one antimicrobial and that 17% (10/59) were resistant to three or more antimicrobials, rates which were lower than the rates found in this study (76% and 36%, respectively).…”

Section: Discussionmentioning

confidence: 88%

“…E. coli has been associated with systemic sepsis (9). E. coli strains from various animal species have been investigated extensively (10)(11)(12)(13)(14). However, little is known about the distribution and characteristics of E. coli in giant pandas.…”

mentioning

confidence: 99%

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

Wang

Yan

Xia

et al. 2013

Appl Environ Microbiol

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f Although Escherichia coli typically colonizes the intestinal tract and vagina of giant pandas, it has caused enteric and systemic disease in giant pandas and greatly impacts the health and survival of this endangered species. In order to understand the distribution and characteristics of E. coli from giant pandas, 67 fecal and 30 vaginal E. coli isolates from 21 giant pandas were characterized for O serogroups, phylogenetic groups, antimicrobial susceptibilities, and pulsed-field gel electrophoresis (PFGE) profiles. In addition, these isolates were tested for the presence of extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC) by multiplex PCR detection of specific virulence genes. The most prevalent serogroups for all E. coli isolates were O88, O18, O167, O4, and O158. ExPEC isolates were detected mostly in vaginal samples, and DEC isolates were detected only in fecal samples. Phylogenetic group B1 predominated in fecal isolates, while groups B2 and D were frequently detected in vaginal isolates. Resistance to trimethoprim-sulfamethoxazole was most frequently observed, followed by resistance to nalidixic acid and tetracycline. All except five isolates were typeable by using XbaI and were categorized into 74 PFGE patterns. Our findings indicate that panda E. coli isolates exhibited antimicrobial resistance, and potentially pathogenic E. coli isolates were present in giant pandas. In addition, these E. coli isolates were genetically diverse. This study may provide helpful information for developing strategies in the future to control E. coli infections of giant pandas.

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Antimicrobial resistances of extraintestinal pathogenic Escherichia coli isolates from swine in China

Cited by 40 publications

References 21 publications

Genome Sequence of a Porcine Extraintestinal Pathogenic Escherichia coli Strain

Genome Sequence of a Porcine Extraintestinal Pathogenic Escherichia coli Strain

TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

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