Antimicrobial resistance, virulence factors, and genotypes of Pseudomonas aeruginosa clinical isolates from Gorgan, northern Iran

Shahri, Fatemeh Nemati; Izanloo, Ahdieh; Goharrizi, Mohammad Ali Sheikh Beig; Jamali, Ailar; Bagheri, Hanieh; Hjimohammadi, Afsaneh; Ardebili, Abdollah

doi:10.1007/s10123-022-00256-7

Cited by 6 publications

(5 citation statements)

References 58 publications

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“…These investigations have revealed significant variations in the enzyme production across different clinical specimens, with hemolysin and protease being the most commonly produced enzymes [ 12 , 13 ]. These findings agree with previous research emphasizing the importance of hemolysin and protease in P. aeruginosa infections [ 14 , 15 ]. In addition, the absence or lower occurrence of certain enzymes, such as chondroitinase and hyaluronidase, in clinical isolates aligns with previous studies that reported the absence of these enzymes in specific strains of P. aeruginosa [ 16 , 17 ].…”

Section: Discussionsupporting

confidence: 93%

Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates

Aqel,

Sannan,

Foudah

et al. 2023

Antibiotics

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(1) Background: This study summarizes the findings of two studies investigating the inhibitory effects of Pseudomonas aeruginosa strains from clinical and environmental sources against gram-positive and gram-negative bacteria and fungi. The studies also analyzed the correlation between enzyme production and inhibitory effects to gain insights into the antimicrobial capabilities of P. aeruginosa strains; (2) Methods: Both studies employed similar methodologies, including the use of disk diffusion and well diffusion methods to assess the inhibitory effects of P. aeruginosa strains against target pathogens. Enzyme production was analyzed through various biochemical assays to determine the diversity and frequencies of enzyme secretion among the strains; (3) Results: A comparative analysis of enzyme production in P. aeruginosa strains from clinical sources revealed significant variations in enzyme production, with hemolysin and protease being the most commonly produced enzymes. Gelatinase production showed lower rates, whereas chondroitinase and hyaluronidase were absent or occurred less frequently. In contrast, a comparative analysis of enzyme production in environmental isolates showed different patterns, indicating adaptation to environmental conditions. Pyocyanin production was absent in all environmental isolates. The inhibitory effects against gram-positive and gram-negative bacteria varied among different P. aeruginosa strains, with strain-specific variations observed. Limited inhibitory effects were observed against fungi, primarily toward gram-positive bacteria; (4) Conclusions: The findings highlight the strain-specific nature of inhibitory effects and enzyme production in P. aeruginosa strains. The correlation between enzyme production and inhibitory effects against gram-positive bacteria suggest a potential role of specific enzymes, such as hemolysin and protease, in the antimicrobial activity. The complexity of the relationship between enzyme production and the inhibition of different pathogens requires further investigation. The results emphasize the potential of P. aeruginosa strains as sources for antimicrobial strategies, particularly against gram-positive bacteria. Future research should focus on understanding the mechanisms underlying these inhibitory effects and exploring their therapeutic applications.

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Section: Discussionsupporting

confidence: 93%

Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates

Aqel,

Sannan,

Foudah

et al. 2023

Antibiotics

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“…Our findings indicate that as well as imposing a fitness cost, colistin resistance can increase susceptibility of some mutants to meropenem and ceftazidime and, in a smaller number of cases, to tobramycin. Of the 232 isolates that were tested in this study, 7.8% were resistant to colistin, a frequency that is similar to or higher than other studies [ 12 – 15 ]. The extent to which the frequency of resistance is affected by the balance between colistin usage and fitness costs in clinical, animal and environmental isolates remains to be determined.…”

Section: Discussionsupporting

confidence: 85%

“…aeruginosa , with up to 10% of P . aeruginosa being resistant in some studies [ 12 – 15 ]. Resistance to an antibiotic can alter susceptibility to other antibiotics and can also affect bacterial fitness [ 16 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…However the development of an aerosilised form that can be inhaled directly to treat lung infections has reduced toxic side effects and it is commonly used in treatment of patients with cystic fibrosis or other forms of bronchiectasis [11]. An increased use of colistin has resulted in the emergence of colistin-resistant P. aeruginosa, with up to 10% of P. aeruginosa being resistant in some studies [12][13][14][15]. Resistance to an antibiotic can alter susceptibility to other antibiotics and can also affect bacterial fitness [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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The PitA protein contributes to colistin susceptibility in Pseudomonas aeruginosa

Erdmann,

Gardner,

Lamont

2023

PLoS ONE

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Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of problematic infections in individuals with predisposing conditions. Infections can be treated with colistin but some isolates are resistant to this antibiotic. To better understand the genetic basis of resistance, we experimentally evolved 19 independent resistant mutants from the susceptible laboratory strain PAO1. Whole genome sequencing identified mutations in multiple genes including phoQ and pmrB that have previously been associated with resistance, pitA that encodes a phosphate transporter, and carB and eno that encode enzymes of metabolism. Individual mutations were engineered into the genome of strain PAO1. Mutations in pitA, pmrB and phoQ increased the minimum inhibitory concentration (MIC) for colistin 8-fold, making the bacteria resistant. Engineered pitA/phoQ and pitA/pmrB double mutants had higher MICs than single mutants, demonstrating additive effects on colistin susceptibility. Single carB and eno mutations did not increase the MIC suggesting that their effect is dependent on the presence of other mutations. Many of the resistant mutants had increased susceptibility to β-lactams and lower growth rates than the parental strain demonstrating that colistin resistance can impose a fitness cost. Two hundred and fourteen P. aeruginosa isolates from a range of sources were tested and 18 (7.8%) were colistin resistant. Sequence variants in genes identified by experimental evolution were present in the 18 resistant isolates and may contribute to resistance. Overall our results identify pitA mutations as novel contributors to colistin resistance and demonstrate that resistance can reduce fitness of the bacteria.

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“…Various detection methods can be applied to capture these signal changes, including fluorescence detection, absorbance measurement, and electrochemical detection [23].…”

Section: Dnase I Sensormentioning

confidence: 99%

Advances in rapid detection of Pseudomonas aeruginosa with DNase-based sensors

Madan,

Chen

2023

BAB

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<p style="text-align: justify;">Pseudomonas aeruginosa is a common pathogen, and its presence in medical environments and water bodies has attracted widespread attention. Traditional detection methods are usually time-consuming and cumbersome, so it is necessary to develop a rapid and sensitive detection technology. DNase can specifically recognize and cut DNA molecules complementary to its substrate sequence. The researchers took advantage of this property to design various DNase-based sensors for detecting the presence of Pseudomonas aeruginosa. These sensors usually use DNase as a recognition element to identify target strains by hybridizing with specific DNA sequences. When the target strain is present, DNase is activated and begins to catalyze the cleavage reaction, producing a detectable signal. This DNase-based sensor has the advantages of rapidity, high sensitivity, and high specificity. In addition, the researchers also explored combining DNase with nanomaterials, fluorescent dyes, etc. to further improve the performance of the sensor. These improvements have improved the detection ability of the sensor in complex samples, laying the foundation for practical applications. With the continuous improvement of technology, these sensors are expected to be widely used in medical, environmental monitoring and other fields, and provide more efficient and convenient solutions for bacterial detection. This study reviewed the research progress of DNase-based sensors for the rapid detection of Pseudomonas aeruginosa.</p>

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Antimicrobial resistance, virulence factors, and genotypes of Pseudomonas aeruginosa clinical isolates from Gorgan, northern Iran

Cited by 6 publications

References 58 publications

Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates

Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates

The PitA protein contributes to colistin susceptibility in Pseudomonas aeruginosa

Advances in rapid detection of Pseudomonas aeruginosa with DNase-based sensors

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