Antimicrobial Resistance Traits of Escherichia coli Isolated from Dairy Manure and Freshwater Ecosystems Are Similar to One Another but Differ from Associated Clinical Isolates

Beattie, Rachelle E.; Bakke, Ellen; Konopek, Nicholas; Thill, Rebecca; Munson, Erik

doi:10.3390/microorganisms8050747

Cited by 9 publications

(12 citation statements)

References 37 publications

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“…Providencia spp. are Gram negative, rod shaped, and facultative anaerobic bacteria which are part of the family Enterobacteriaceae (Beattie et al, 2020;Esperón et al, 2020;Massé et al, 2021). The well-studied species include P. stuartii, P. alcalifaciens, and, P. rettgeri with these bacteria linked to gastroenteritis, Urinary Tract Infections (UTIs) and bacteraemia (Holman et al, 2016;Chuppava et al, 2019;Esperón et al, 2020;Yoshizawa et al, 2020;Congilosi and Aga, 2021).…”

Section: Providencia Sppmentioning

confidence: 99%

“…Information as to their infection of farm animals is limited, however, the genus has been cited as exhibiting high levels of antimicrobial resistance (AMR) (Esperón et al, 2020) and while infection by Providencia spp. is rare, there are potential reservoirs of ARGs in the wider environment (Beattie et al, 2020). It would be beneficial for future studies to investigate the prevalence the impact of Providencia spp.…”

Section: Providencia Sppmentioning

confidence: 99%

“…E. coli isolated from manure and environmental samples are considerably different from the clinical isolates, which were reported to show resistance patterns against ampicillin, ceftazidime, ceftriaxone, ciprofloxacin, gentamicin, trimethoprim-sulfamethoxazole and levofloxacin (Beattie et al, 2020). Compared to more virulent clinical E. coli isolates, those isolated from manure and environmental samples also harbor the plasmids and the potential to develop biofilms responsible for producing a plethora of ARGs (Beattie et al, 2020).…”

Section: Manure As a Vector Of Antimicrobial Resistancementioning

confidence: 99%

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The Fate of Foodborne Pathogens in Manure Treated Soil

et al. 2021

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The aim of this review was to provide an update on the complex relationship between manure application, altered pathogen levels and antibiotic resistance. This is necessary to protect health and improve the sustainability of this major farming practice in agricultural systems based on high levels of manure production. It is important to consider soil health in relation to environment and land management practices in the context of the soil microflora and the introduction of pathogens on the health of the soil microbiome. Viable pathogens in manure spread on agricultural land may be distributed by leaching, surface run-off, water source contamination and contaminated crop removal. Thus it is important to understand how multiple pathogens can persist in manures and on soil at farm-scale and how crops produced under these conditions could be a potential transfer route for zoonotic pathogens. The management of pathogen load within livestock manure is a potential mechanism for the reduction and prevention of outbreaks infection with Escherichia coli, Listeria Salmonella, and Campylobacter. The ability of Campylobacter, E. coli, Listeria and Salmonella to combat environmental stress coupled with their survival on food crops and vegetables post-harvest emphasizes the need for further study of these pathogens along with the emerging pathogen Providencia given its link to disease in the immunocompromised and its’ high levels of antibiotic resistance. The management of pathogen load within livestock manure has been widely recognized as a potential mechanism for the reduction and prevention of outbreaks infection but any studies undertaken should be considered as region specific due to the variable nature of the factors influencing pathogen content and survival in manures and soil. Mediocre soils that require nutrients could be one template for research on manure inputs and their influence on soil health and on pathogen survival on grassland and in food crops.

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Section: Providencia Sppmentioning

confidence: 99%

Section: Providencia Sppmentioning

confidence: 99%

Section: Manure As a Vector Of Antimicrobial Resistancementioning

confidence: 99%

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The Fate of Foodborne Pathogens in Manure Treated Soil

et al. 2021

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“…In areas that release treated wastewater into local receiving waters accessed by humans and animals, this evaluation is vital to reduce potential health risks. Previous studies by our labs have characterized antibiotic resistance phenotypes and genotypes of E. coli from manure, wastewater, sediment and surface waters, hospital wastewater, and clinical strains [ 10 , 18 , 19 ]. This study expands on our previous findings through the following aims: (i) to assess the presence of cefotaxime (CTX)-resistant Escherichia coli populations in hospital and urban WW; (ii) to compare resistance patterns among clinical ESBL-producing E. coli to urban and hospital WW strains within a defined geographical location; and (iii) to further assess the presence of virulence factors among cefotaxime resistant populations to provide a more complete public health risk analysis.…”

Section: Introductionmentioning

confidence: 99%

Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant Escherichia coli Wastewater and Clinical Isolates

et al. 2022

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The World Health Organization has identified antibiotic resistance as one of the largest threats to human health and food security. In this study, we compared antibiotic resistance patterns between ESBL-producing Escherichia coli from human clinical diseases and cefotaxime-resistant environmental strains, as well as their potential to be pathogenic. Antibiotic susceptibility was tested amongst clinical isolates (n = 11), hospital wastewater (n = 22), and urban wastewater (n = 36, both influent and treated effluents). Multi-drug resistance predominated (>70%) among hospitalwastewater and urban wastewater influent isolates. Interestingly, isolates from clinical and urban treated effluents showed similar multi-drug resistance rates (~50%). Most hospital wastewater isolates were Phylogroup A, while clinical isolates were predominately B2, with a more diverse phylogroup population in urban wastewater. ESBL characterization of cefotaxime-resistant populations identified blaCTX-M-1 subgroup as the most common, whereby blaKPC was more associated with ceftazidime and ertapenem resistance. Whole-genome sequencing of a carbapenemase-producing hospital wastewater E. coli strain revealed plasmid-mediated blaKPC-2. Among cefotaxime-resistant populations, over 60% of clinical and 30% of treated effluent E. coli encoded three or more virulence genes exhibiting a pathogenic potential. Together, the similarity among treated effluent E. coli populations and clinical strains suggest effluents could serve as a reservoir for future multi-drug resistant E. coli clinical infections.

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“…One of the published studies aimed to compare antimicrobial resistance (AMR) in extended-spectrum cephalosporin-resistant and generic E. coli from a One Health approach applied on a beef production system, identifying the municipal sewage as a hot spot for MDR emergence and dissemination [ 10 ]. Also published was a study that identified associations in resistance traits between E. coli isolated from clinical, dairy manure, and freshwater ecosystem environments concluding that manure and environmental isolates were significantly different from clinical isolates based on analyzed traits, suggesting more transmission occurs between these two sources in the sampled environment [ 11 ]. The same approach was held by a different study aiming at detecting several virulence factors genes (fimA, papC, papG III, cnf1, hlyA and aer) and to determine the conjugative capacity in a wide collection of ESBL-producing E. coli isolated from different sources (human, food, farms, rivers, and wastewater treatment plants), emphasizing the need of a specific surveillance program of AMR indicators in wastewaters from animal or human origin, in order to apply sanitary measures to reduce the burden of resistant bacteria arriving to risky environments such as WWTPs [ 12 ].…”

mentioning

confidence: 99%