Antimicrobial resistance Salmonella isolates recovered from food products of animal origin in the Russian Federation

Abstract

The study was aimed at Salmonella isolation from samples of animal food products submitted for testing from various regions of the Central part of the RF and serotyping of the recovered isolates and their testing for antibiotic resistance. A total of 2,342 tests were performed and 87 (3.7%) Salmonella isolates were recovered. Most of them (54 isolates) were recovered from poultry meat and poultry meat preparation samples submitted for testing. Besides, 25 isolates were recovered from pork and pork preparation samples, 7 isolates – from beef samples, 1 isolate – from hard cheese samples. Serotyping of 64 Salmonella isolates showed that the majority of the isolates (57.8%) belonged to О7 group. Also, Salmonella isolates belonging to О9 (21.9%), О8 (9.4%), О4,5 (6.2%) and О10 (4.7%) were detected in food products. S. Enteritidis, (23.3%), and S. infantis (18.7%), were predominant based on the number of detections. Also, the following serovars were identified: S. typhimurium, S. nigeria, S. montevideo, S. derby, S. meleagridis, S. virchov, S. oranienburg. Tests of 87 Salmonella isolates for their antibiotic resistance with disk diffusion method revealed that they were highly resistant to nalidixic acid (70.1%), tetracycline (49.4%), trimethoprim/sulfamethoxazol (40.2%). Moreover, nalidixic acid-resistance was common for all identified isolates. Seventeen isolates (19.5%) demonstrated multiple antibiotic resistance and two isolates were found to be resistant to ≥7 antibiotics. All recovered isolates were susceptible to gentamicin, amikacin, meropenem and imipenem. Obtained results indicate the necessity of Salmonella antibiotic resistance monitoring to gain understanding of Salmonellas’ antibiotic resistance emergence and trends.