Antimicrobial resistance profiling and molecular typing of ruminant-borne isolates of Clostridium perfringens from Xinjiang, China

Wang, Xiaoting; Ning, Chengcheng; Ji, Chunhui; Li, Yan; Li, Jing; Meng, Qingling; Qiao, Jun; Wang, Lixia; Kuojun, Cai; Zhang, Jinsheng; Zhang, Zaichao; Yu, Weiwu; Yelong, Peng; Xue-peng, Cai

doi:10.1016/j.jgar.2021.08.003

Cited by 8 publications

(9 citation statements)

References 18 publications

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“…MLST analysis revealed that 186 C. perfringens isolates exhibited 135 sequence types (STs) with a high degree of genetic diversity, including 93 new STs. In the segments of colA (18), groEL (16), sodA (20), plc (23), gyrB (13), sigK (10), pgk (9), and nadA (20), up to 129 new alleles were identified, resulting in new genotypes. Among the 132 STs, the most frequent ST was ST221 (7/186, 3.74%), followed by ST408 (5/186, 2.67%), ST479 (5/186, 2.67%), ST62 (4/186, 2.14%), and ST493 (4/186, 2.14%).…”

Section: Resultsmentioning

confidence: 99%

Section: Evolution and Phylogenetic Analysis Of C Perfringens Isolate...mentioning

confidence: 99%

“…The copyright holder for this this version posted September 24, 2022. ; https://doi.org/10.1101/2022.09. 23.509295 doi: bioRxiv preprint 8). Recently, an increase in C. perfringens antimicrobial resistance has been noted, and some investigations have indicated that most C. perfringens strains of animal origin are multidrug resistant (MDR) (9,10).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Zhong

Zheng

Wang

et al. 2022

Preprint

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Clostridium perfringens (C. perfringens) is a significant foodborne pathogen and a common cause of intestinal diseases in both animals and humans. Altogether, 186 isolates were obtained from humans (n = 147), animals (n = 25), and food (n = 14), comprising 174 type A strains (93.55%), 11 type F strains (5.91%), and one type D strain (0.54%); and were analyzed by multilocus sequence typing (MLST) and antimicrobial susceptibility testing. Additionally, some specific ST complexes were analyzed by cgMLST and cgSNP to investigate genetic relatedness. MLST indicated the most prevalent STs of C. perfringens of human and animal origin were as follows: ST221 (5/147), ST62 (4/147), ST408 (4/147), and ST493 (4/147) were predominant in humans, while ST479 (5/25) was the major type in animals. Within the same ST complex, genetically unrelated relationships or potential clustering/transmission events were further recognized by cgMLST and cgSNP, illustrating that these two methods are valuable in defining outbreaks and transmission events. All tested isolates were susceptible to vancomycin and meropenem. The rates of resistance to metronidazole, penicillin, cefoxitin, moxifloxacin, and clindamycin were low (metronidazole: 1.08%; penicillin: 9.68%; cefoxitin: 0.54%; moxifloxacin: 6.45%; and chloramphenicol: 3.76%). Interestingly, 49.66% of human origin were clindamycin-resistant, and 18.2% were penicillin-insensitive. Importantly, the portion of MDR isolates was significantly lower than in previous reports. The study provides an overview of the epidemiological characteristics of C. perfringens with different origins and hosts in China. C. perfringens demonstrated remarkable genetic diversity and distinct molecular features compared to antibiotic-resistance profiles from other studies.

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Section: Resultsmentioning

confidence: 99%

Section: Evolution and Phylogenetic Analysis Of C Perfringens Isolate...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Zhong

Zheng

Wang

et al. 2022

Preprint

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“…Furthermore, ST19 has been found mostly in human clinical Salmonella isolates, but also in animals and the environment, and successful in South African and China. ST19 was only occasionally found in United States and Mexico and coexists with quinolone resistance genes qnrS (Kariuki and Onsare, 2015;Gómez-Baltazar et al, 2019;Monte et al, 2020;Yao et al, 2020;Xiaoting et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of blaCTX-M-Positive Salmonella Typhimurium From Diarrhoeal Outpatients in Guangdong, China, 2010–2017

Jiang

et al. 2022

Front. Microbiol.

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Salmonella enterica can lead to intestinal diarrhea, and the emergence and spread of cephalosporin-resistant Salmonella have brought great challenges to clinical treatment. Therefore, this study investigated the prevalence and transmission of blaCTX-M genes among S. Typhimurium from diarrhoeal outpatients in Guangdong, China, from 2010 to 2017. A total of 221 blaCTX-M-positive isolates were recovered from 1,263 S. Typhimurium isolates from the facal samples of diarrhoea patients in 45 general hospitals from 11 cities. The most popular CTX-M gene was blaCTX-M-55 (39.6%, 72/182) in the CTX-M-1 group, followed by blaCTX-M-14 (22.5%, 41/182) and blaCTX-M-65 (19.2%, 35/182) in the CTX-M-9 group. The isolates that carried blaCTX-M-9G had significantly higher resistance rates to multiple antibacterials compared with blaCTX-M-1G (p < 0.01). Meanwhile, PFGE analysis not only showed the clonal transmission of blaCTX-M-55/14/65-positve isolates of diarrhoeal outpatients’ origins from different hospitals in Guangdong province, but also the characteristic of blaCTX-M-55/14/65-positve isolates’ bacterial persistence. Multilocus sequence typing (MLST) analysis indicated that these S. Typhimurium isolates possessed ST34 and ST19. Furthermore, genomic Beast phylogenomic analysis provided the evidence of a close relationship of blaCTX-M-positive S. Typhimurium isolates between the outpatients and pork. Most blaCTX-M-55/14/65 genes were transmitted by non-typeable or IncI1/IncFII/IncHI2 plasmids with the size of ranging from ~80 to ~280 kb. Moreover, whole-genome sequencing (WGS) analysis further revealed that blaCTX-M-55/14/65 coexisted with other 25 types of ARGs, of which 11 ARGs were highly prevalent with the detection rates >50%, and it first reported the emergence of blaTEM-141 in S. Typhimurium. This study underscores the importance of surveillance for blaCTX-M-positive microbes in diarrhea patients.

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“…Due to the large societal and economic impact of Cp and Cd infections, and concerns regarding increasing antibiotic resistance related to Cp ( Xiaoting et al, 2021 ) and Cd ( Imwattana et al, 2019 ), effective treatment and prevention strategies are in high demand. Among those, the use of bacteriophages (phage therapy) and phage-derived endolysins to treat bacterial infections has attracted recent attention.…”

Section: Introductionmentioning

confidence: 99%

Battling Enteropathogenic Clostridia: Phage Therapy for Clostridioides difficile and Clostridium perfringens

2022

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The pathogenic Clostridioides difficile and Clostridium perfringens are responsible for many health care-associated infections as well as systemic and enteric diseases. Therefore, they represent a major health threat to both humans and animals. Concerns regarding increasing antibiotic resistance (related to C. difficile and C. perfringens) have caused a surge in the pursual of novel strategies that effectively combat pathogenic infections, including those caused by both pathogenic species. The ban on antibiotic growth promoters in the poultry industry has added to the urgency of finding novel antimicrobial therapeutics for C. perfringens. These efforts have resulted in various therapeutics, of which bacteriophages (in short, phages) show much promise, as evidenced by the Eliava Phage Therapy Center in Tbilisi, Georgia (https://eptc.ge/). Bacteriophages are a type of virus that infect bacteria. In this review, the (clinical) impact of clostridium infections in intestinal diseases is recapitulated, followed by an analysis of the current knowledge and applicability of bacteriophages and phage-derived endolysins in this disease indication. Limitations of phage and phage endolysin therapy were identified and require considerations. These include phage stability in the gastrointestinal tract, influence on gut microbiota structure/function, phage resistance development, limited host range for specific pathogenic strains, phage involvement in horizontal gene transfer, and—for phage endolysins—endolysin resistance, -safety, and -immunogenicity. Methods to optimize features of these therapeutic modalities, such as mutagenesis and fusion proteins, are also addressed. The future success of phage and endolysin therapies require reliable clinical trial data for phage(-derived) products. Meanwhile, additional research efforts are essential to expand the potential of exploiting phages and their endolysins for mitigating the severe diseases caused by C. difficile and C. perfringens.

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Antimicrobial resistance profiling and molecular typing of ruminant-borne isolates of Clostridium perfringens from Xinjiang, China

Cited by 8 publications

References 18 publications

Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Epidemiology of blaCTX-M-Positive Salmonella Typhimurium From Diarrhoeal Outpatients in Guangdong, China, 2010–2017

Battling Enteropathogenic Clostridia: Phage Therapy for Clostridioides difficile and Clostridium perfringens

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