Antimicrobial resistance profiling andfla-typing of Irish thermophillicCampylobacterspp. of human and poultry origin

Abstract

Aims: To determine the genetic relationship between a random collection of Irish human and poultry Campylobacter isolates and to determine the frequency of antibiotic resistance. Methods and Results: Sixty‐six Campylobacter isolates (34 human and 32 poultry) were typed by restriction fragment length polymorphism of flagellin‐A (flaA) PCR products (flaA‐RFLP) and by sequencing of the short variable region of flaA (flaA‐SVR). FlaA‐RFLP identified 58 distinct profiles, while flaA‐SVR identified 28 different alleles. The highest level of antibiotic resistance was found for ampicillin (48·5%) followed by nalidixic acid (42·2%) and ciprofloxacin (31·8%). In general, poultry isolates displayed a higher incidence of resistance to the antimicrobials tested than the human isolates. Conclusions: A high level of genetic diversity existed among the Campylobacter strains confirming the weak clonality of this species. There was no relationship between antimicrobial resistance and specific genotypes determined by flaA typing suggesting that resistance was due to pressures of antimicrobial therapy. Significance and Impact of the Study: This study emphasizes the need for increased surveillance of antimicrobial susceptibility among Campylobacter isolates in Ireland and the implementation of more stringent control policies relating to the use of antimicrobials in the poultry industry.

Characterization of antimicrobial resistance and application of RFLP for epidemiological monitoring of thermophilic Campylobacter spp. isolated from dogs and humans in Korea

“…This result agrees with the previous studies which suggested that none of C. jejuni isolates from humans and companion animals was resistant [16,28]. However, other studies reported 3-6.8% of erythromycin resistance in C. jejuni isolated from humans [7,18].…”

“…can be recognized as a major public health concern [7,23]. In this study, the prevalence of antimicrobial resistance in Campylobacter spp.…”

β-Lactamase-Mediated β-Lactam Resistance in Campylobacter Species: Prevalence of Cj0299 ( bla OXA-61 ) and Evidence for a Novel β-Lactamase in C . jejuni

“…tested by participating laboratories worldwide during 2005 to 2006, 26.5% were resistant to ampicillin. Corcoran et al (4) reported that ampicillin resistance was prevalent (48.5%) among Irish isolates of Campylobacter spp. and that the incidence of antimicrobial resistance in poultry isolates was higher than in those from humans.…”

fla-typing, RAPD analysis, isolation rate and antimicrobial resistance profile of Campylobacter jejuni and Campylobacter coli of human origin collected from hospitals in Tehran, Iran

“…are considered as one of the major causative agents of acute gastro-enteritis in both humans and animals (Al-Mahmeed et al 2006;Allos 2001;Corcoran et al 2006;Feodoroff et al 2009). The clinical manifestations of the enteritis caused by Campylobacter spp.…”