Antimicrobial Resistance Profiles of<i>Enterococcus faecalis</i>and<i>Enterococcus faecium</i>Isolated from Artisanal Food of Animal Origin in Argentina

Delpech, Gastón; Pourcel, Gisela; Schell, Celia María; Luca, María De; Basualdo, Juan Ángel; Bernstein, Judith Celina; Grenóvero, Silvia; Sparo, Mónica

doi:10.1089/fpd.2012.1192

Cited by 25 publications

(26 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, at the same time of avoparcin ban in the EU, vancomycin-resistant E. faecium were not isolated from animal food products in the US [16,17]. In Argentina, Delpech et al [18] recovered vancomycin-resistant E. faecium from artisanal salami, cow cheese and goat cheese.…”

Section: Introductionmentioning

confidence: 99%

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Pourcel¹,

Sparo²,

Alej³

et al. 2017

Clin Microbiol

Self Cite

View full text Add to dashboard Cite

Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n:13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed among seven clonal types; esp gene was detected in clinical strains. There was no clonal relationship with food vanA E. faecium, but these strains could pose a risk in intra/inter genus transfer of vanA determinant to human-adapted strains. High-level gentamicin resistant E. faecalis (n:7) were recovered from human and food samples. Glycopeptide resistance was not observed; cylA gene was detected in most of the clinical high-level gentamicin resistant E. faecalis isolates. PFGE patterns showed four clonal types in high-level gentamicin resistant E. faecalis strains; there was demonstrated clonal relatedness between isolates from different origin. In Argentina, this is the first study showing a clonal relationship between high-level gentamicin resistant E. faecalis isolated from food and humans. These results encourage the study of dissemination of clonal complexes with mobile resistance genes.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Pourcel¹,

Sparo²,

Alej³

et al. 2017

Clin Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the expression of virulence genes is mainly related to quorum sensing. Further studies must be performed to examine whether the E. faecalis isolates in this study are pathogenic (Huebner et al, 1999; Delpech et al, 2012). All the 58 collected E. faecalis isolates were cylA -negative and hyl -negative, which is not consistent with previous studies (Creti et al, 2004; Medeiros et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Genetic Diversity of Enterococcus faecalis Isolates from Mineral Water and Spring Water in China

Lei

Zhang

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

Enterococcus faecalis is an important opportunistic pathogen which is frequently detected in mineral water and spring water for human consumption and causes human urinary tract infections, endocarditis and neonatal sepsis. The aim of this study was to determine the prevalence, virulence genes, antimicrobial resistance and genetic diversity of E. faecalis from mineral water and spring water in China. Of 314 water samples collected from January 2013 to January 2014, 48 samples (15.3%) were contaminated E. faecalis. The highest contamination rate occurred in activated carbon filtered water of spring water (34.5%), followed by source water of spring water (32.3%) and source water of mineral water (6.4%). The virulence gene test of 58 E. faecalis isolates showed that the detection rates of asa1, ace, cylA, gelE and hyl were 79.3, 39.7, 0, 100, 0%, respectively. All 58 E. faecalis isolates were not resistant to 12 kinds of antibiotics (penicillin, ampicillin, linezolid, quinupristin/dalfopristin, vancomycin, gentamicin, streptomycin, ciprofloxacin, levofloxacin, norfloxacin, nitrofurantoin, and tetracycline). Enterobacterial repetitive intergenic consensus-PCR classified 58 isolates and three reference strains into nine clusters with a similarity of 75%. This study is the first to investigate the prevalence of E. faecalis in mineral water and spring water in China. The results of this study suggested that spring water could be potential vehicles for transmission of E. faecalis.

show abstract

“…Resistance to vancomycin is increased among Enterococci in different countries (15). Although a lot of efforts have been concentrated on studying vancomycin high level resistance Enterococcus, there are very little published studies on VLRE (16)(17)(18). VLRE infections are posing a serious problem in healthcare settings because their detection is difficult and treatment of these infections may not be successful because these species are miss-identified as vancomycin susceptible (19)(20)(21).…”

Section: Discussionmentioning

confidence: 99%

“…Teicoplanin resistance patterns in VRE have been reported to depend on presence of vanA or vanB genes (18,24).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of VanA and B Genotype Among Vancomycin Low Resistant Enterococcus in Fecal Normal Flora and Clinical Samples Isolated From Tehran’s Hospitals

Teymournejad¹,

Mobarez²,

Doust³

et al. 2015

Int J Enteric Pathog

View full text Add to dashboard Cite

Background: Vancomycin-Resistant Enterococci (VRE) pose an emerging problem in Iran hospitals. Objectives: The present study was undertaken to determine the prevalence of van genes among vancomycin low resistant Enterococccus (VLRE) isolate in Tehran hospitals. Materials and Methods: Totally 162 and 152 isolates of enterococcal species were obtained from fecal and clinical samples of hospitalized patients between March and November of 2012. The antibiotic susceptibility of the isolates and minimum inhibitory concentration (MIC) were determined according to CLSI. The presence of the van A and B resistant genes in VLREs isolates were evaluated by PCR method. Results: VLRE accounted for 162 (38.4%) and 159 (38.7%) of Enterococal isolates from clinical and fecal flora samples respectively (MIC's in the range of 16 to 64 µg/mL). VanA and vanB genotypes were detected with polymerase chain reaction (PCR) in 85 (27%) and 35 (11%) of VLRE isolates, respectively. Conclusions: VLRE cause serious problems in healthcare settings because their detection is difficult and treatment of these infections may not be successful. These species are miss-identified as vancomycin susceptible isolates. By detection of VLRE, we can evaluate perspective of vancomycin high level resistant Enterococcus rate in future.

show abstract

Antimicrobial Resistance Profiles ofEnterococcus faecalisandEnterococcus faeciumIsolated from Artisanal Food of Animal Origin in Argentina

Cited by 25 publications

References 36 publications

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Prevalence and Genetic Diversity of Enterococcus faecalis Isolates from Mineral Water and Spring Water in China

Prevalence of VanA and B Genotype Among Vancomycin Low Resistant Enterococcus in Fecal Normal Flora and Clinical Samples Isolated From Tehran’s Hospitals

Contact Info

Product

Resources

About