Antimicrobial resistance profile of<i>Aeromonas</i>species isolated from Libya

Ghenghesh, Khalifa Sifaw; El-Mohammady, Hanan; Levin, Samuel Y.; Zorgani, Abdulazziz; Tawil, Khaled

doi:10.3402/ljm.v8i0.21320

Cited by 19 publications

(19 citation statements)

References 8 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In isolates from human samples, especially resistance to amoxicillin, amoxicillin-clavulanic acid, cefazolin cephalothin, colistin, nalidixic acid, trimethoprim-sulphamethoxazole and tetracyclines was detected (Aravena-Roman et al, 2012;Ghenghesh et al, 2013;Soltan Dallal et al, 2016). Many isolates showed multi-resistance to various antibiotic substances (Piotrowska & Popowska, 2014).…”

Section: Discussionsupporting

confidence: 51%

“…from fish were resistant to ampicillin, amoxicillin-clavulanic acid, piperacillin, ticarcillin-clavulanic acid, ceftriaxone, ceftazidime, ceftriaxone, cefotaxime, cefepime, aztreonam, imipenem, amikacin, netilmicin and chloramphenicol (Dias et al, 2012). In food samples, aeromonads resistant to amoxicillin, ampicillin, bacitracin, cefadroxil, cloxacillin, erythromycin, kanamycin, methicillin, neomycin, novobiocin, oxacillin, penicillin, polymyxin-B, rifampicin, tetracyclines, trimethoprimsulphamethoxazole and vancomycin are described (Ghenghesh et al, 2013;Stratev & Odeyemi, 2016;Vivekanandhan et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Diagnostic methods for identifying different Aeromonas species and examining their pathogenicity factors, their correlation to cytotoxicity and adherence to fish mucus

Jung‐Schroers

Jung

Ryll

et al. 2018

Journal of Fish Diseases

View full text Add to dashboard Cite

Aeromonas spp. are ubiquitous in the aquatic environment, acting as facultative or obligate pathogens for fish. Identifying Aeromonas spp. is important for pathogenesis and prognosis in diagnostic cases but can be difficult because of their close relationship. Forty‐four already characterized isolates of Aeromonas spp. were analysed by 16S rRNA gene sequencing, by gyrase B sequencing, by analysing their fatty acid profiles, by biochemical reactions and by MALDI‐TOF MS. To determine their pathogenicity, cytotoxicity, adhesion to mucus and the expression of 12 virulence factors were tested. The susceptibility of the isolates towards 13 different antibiotics was determined. MALDI‐TOF MS was found to be an acceptable identification method for Aeromonas spp. Although the method does not detect all species correctly, it is time‐effective and entails relatively low costs and no other methods achieved better results. A high prevalence of virulence‐related gene fragments was detected in almost all examined Aeromonas spp., especially in A. hydrophila and A. salmonicida, and most isolates exhibited a cytotoxic effect. Single isolates of A. hydrophila and A. salmonicida showed multiple resistance to antibiotics. These results might indicate the potentially pathogenic capacity of Aeromonas spp., suggesting a risk for aquatic animals and even humans, given their ubiquitous nature.

show abstract

Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Diagnostic methods for identifying different Aeromonas species and examining their pathogenicity factors, their correlation to cytotoxicity and adherence to fish mucus

Jung‐Schroers

Jung

Ryll

et al. 2018

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…Results of the present study are also in tune with the findings of Sharma et al (2010) who isolated ciprofloxacin and amikacin sensitive Aeromonas strains from various foods of animal origin including fish. Ghenghesh et al (2013) recorded significantly higher tetracycline resistance in aeromonads from chicken carcass (33%) in contrast with the present study. Tetracyclines are common additives in poultry feed which can contribute to acquired resistance in poultry.…”

Section: Discussioncontrasting

confidence: 99%

Prevalence of antimicrobial resistant Aeromonas in chicken and fish washings

Pratibha¹,

Rahul²,

Padmakar³

et al. 2014

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

This study was undertaken to assess prevalence of virulent and antibiotic-resistant Aeromonas species in chicken meat and fresh water fish washings procured from local market. Isolation was done on three selective agar viz. Aeromonas isolation media, ampicillin dextrin agar and Aeromonas starch DNA agar. Presumptive colonies were directly screened by multiplex polymerase chain reaction (PCR) targeting genus specific 16S rRNA gene, aerolysin (aerA) of Aeromonas hydrophila and hemolysin (asa1) gene of Aeromonas sobria. Of the 200 samples (100 each of chicken and fish washings), 21 isolates were confirmed as Aeromonas species. We could not detect aer A, however, asa1 of A. sobria was detected in six (28.57%) fish isolates. Aeromonas isolates exhibited 100% resistant to amoxicillin, ampicillin and 95.23% to carbenicillin. Moderate sensitivity was observed to kanamycin (90.47%) and neomycin (71.42%). Isolates were 100% sensitive to gentamicin and ciprofloxacin. Maximum sensitivity was recorded with chloramphenicol, tobramycin (95.23% each) and amikacin (80.95%). PCR characterization revealed presence of class 1 integron and Tet (C) genes in six and 10 isolates, respectively. PSE-1 β-lactamase was not detected in any of the isolates. This study demonstrate the incidence of antimicrobial resistant Aeromonas in chicken and fish environment, which may be a potential source of spread of this enteropathogen in food chain.

show abstract

“…Moreover, previous studies reported 100% Aeromonas resistance rates to ampicillin (Aoki, Egusa, Ogata, & Watanabe, ; Igbinosa, ; Rall et al, ). Most A. hydrophila isolates have intrinsic or chromosomally mediated resistance to ampicillin (Ghenghesh, El‐Mohammady, Levin, Zorgani, & Tawil, ; Rall et al, ).…”

Section: Discussionmentioning

confidence: 99%

Proteomic characterization and discrimination of Aeromonas species recovered from meat and water samples with a spotlight on the antimicrobial resistance of Aeromonas hydrophila

et al. 2019

View full text Add to dashboard Cite

Aeromonas is recognized as a human pathogen following ingestion of contaminated food and water. One major problem in Aeromonas identification is that certain species are phenotypically very similar. The antimicrobial resistance is another significant challenge worldwide. We therefore aimed to use mass spectrometry technology for identification and discrimination of Aeromonas species and to screen the antimicrobial resistance of Aeromonas hydrophila (A. hydrophila). A total of 150 chicken meat and water samples were cultured, and then, the isolates were identified biochemically by the Vitek® 2 Compact system. Proteomic identification was performed by MALDI‐TOF MS and confirmed by a microchannel fluidics electrophoresis assay. Principal component analysis (PCA) and single‐peak analysis created by MALDI were also used to discriminate the Aeromonas species. The antimicrobial resistance of the A. hydrophila isolates was determined by Vitek® 2 AST cards. In total, 43 samples were positive for Aeromonas and comprised 22 A. hydrophila, 12 Aeromonas caviae (A. caviae), and 9 Aeromonas sobria (A. sobria) isolates. Thirty‐nine out of 43 (90.69%) Aeromonas isolates were identified by the Vitek® 2 Compact system, whereas 100% of the Aeromonas isolates were correctly identified by MALDI‐TOF MS with a score value ≥2.00. PCA successfully separated A. hydrophila, A. caviae and A. sobria isolates into two groups. Single‐peak analysis revealed four discriminating peaks that separated A. hydrophila from A. caviae and A. sobria isolates. The resistance of A. hydrophila to antibiotics was 95.46% for ampicillin, 50% for cefotaxime, 45.45% for norfloxacin and pefloxacin, 36.36% for ceftazidime and ciprofloxacin, 31.81% for ofloxacin and 27.27% for nalidixic acid and tobramycin. In conclusion, chicken meat and water were tainted with Aeromonas spp., with a high occurrence of A. hydrophila. MALDI‐TOF MS is a powerful technique for characterizing aeromonads at the genus and species levels. Future studies should investigate the resistance of A. hydrophila to various antimicrobial agents.

show abstract

Antimicrobial resistance profile ofAeromonasspecies isolated from Libya

Cited by 19 publications

References 8 publications

Diagnostic methods for identifying different Aeromonas species and examining their pathogenicity factors, their correlation to cytotoxicity and adherence to fish mucus

Diagnostic methods for identifying different Aeromonas species and examining their pathogenicity factors, their correlation to cytotoxicity and adherence to fish mucus

Prevalence of antimicrobial resistant Aeromonas in chicken and fish washings

Proteomic characterization and discrimination of Aeromonas species recovered from meat and water samples with a spotlight on the antimicrobial resistance of Aeromonas hydrophila

Contact Info

Product

Resources

About