Antimicrobial resistance patterns and genotypic diversity between clinical and water systems isolates of Pseudomonas aeruginosa in Cali, Colombia

Chávez, Mónica; Cabezas, A F; Ferauds, M; Castillo, Jorge Enrique; Caicedo, Luis A.

doi:10.47665/tb.37.3.650

Cited by 3 publications

(2 citation statements)

References 24 publications

(41 reference statements)

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“…Within isolated environmental samples, it was observed that MDR microorganisms are present in environments such as wastewater treatment plants, as was the case for p3, which showed resistance to aztreonam, ciprofloxacin, and amikacin. However, no ST associated with HRC was found, which is a finding that has been previously described in other studies both inside and outside of Colombia [48][49][50][51]. These studies highlight the dissemination of microorganisms through water sources, which could spread bacterial resistance.…”

Section: Discussionsupporting

confidence: 79%

Genomic Differences Associated with Resistance and Virulence in Pseudomonas aeruginosa Isolates from Clinical and Environmental Sites

Aroca Molina,

Gutiérrez,

Benítez-Campo

et al. 2024

Microorganisms

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Pseudomonas aeruginosa is a pathogen that causes healthcare-associated infections (HAIs) worldwide. It is unclear whether P. aeruginosa isolated from the natural environment has the same pathogenicity and antimicrobial resistance potential as clinical strains. In this study, virulence- and resistance-associated genes were compared in 14 genomic sequences of clinical and environmental isolates of P. aeruginosa using the VFDB, PATRIC, and CARD databases. All isolates were found to share 62% of virulence genes related to adhesion, motility, secretion systems, and quorum sensing and 72.9% of resistance genes related to efflux pumps and membrane permeability. Our results indicate that both types of isolates possess conserved genetic information associated with virulence and resistance mechanisms regardless of the source. However, none of the environmental isolates were associated with high-risk clones (HRCs). These clones (ST235 and ST111) were found only in clinical isolates, which have an impact on human medical epidemiology due to their ability to spread and persist, indicating a correlation between the clinical environment and increased virulence. The genomic variation and antibiotic susceptibility of environmental isolates of P. aeruginosa suggest potential biotechnological applications if obtained from sources that are under surveillance and investigation to limit the emergence and spread of antibiotic resistant strains

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Section: Discussionsupporting

confidence: 79%

Genomic Differences Associated with Resistance and Virulence in Pseudomonas aeruginosa Isolates from Clinical and Environmental Sites

Aroca Molina,

Gutiérrez,

Benítez-Campo

et al. 2024

Microorganisms

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“…All the above underlines the need to improve our understanding of how antibiotic resistance develops and spreads in humans, animals, and the environment [ 17 ]. Although in Colombia these types of reports have focused mainly on the clinical perspective [ 18 ] because of a direct relationship between them, in the country there have also been studies of bacterial resistance to antibiotics in rivers and wastewater [ 19 , 20 ], in wastewater treatment plants [ 21 , 22 , 23 ] and in drinking water [ 24 ]. However, there are no studies that have characterized bacteria from landfill leachates and their association with antibiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

Beta-Lactam-Resistant Enterobacterales Isolated from Landfill Leachates

et al. 2022

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Antibiotic resistance is one of the main challenges worldwide due to the high morbidity and mortality caused by infections produced by resistant bacteria. In Colombia, this problem has been studied mainly from the clinical perspective; however, it is scarcely studied in the leachates produced in landfills. The objective of this study was to detect, identify and determine the antibiotic sensitivity profile of Enterobacterales isolated from a leachate treatment plant located in Cali, Colombia. Detection was performed using selective culture media, bacterial identification using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF, bioMérieux) and by sequencing the gene coding for the 16S ribosomal RNA subunit when discrepancies were observed between phenotypic characteristics and MALDI-TOF. Antibiotic sensitivity profiling was determined using the automated VITEK®2 system (bioMérieux). Twenty-one isolates were obtained, of which Klebsiella pneumoniae was the most frequent (23.8%), and 34% of the isolates showed decreased sensitivity to beta-lactam antibiotics such as cefoxitin, ampicillin/sulbactam and piperacillin/tazobactam. These findings suggest that leachates from landfills could be a reservoir of pathogenic bacteria carrying antibiotic resistance determinants, so periodic microbiological characterization of these effluents should be performed, promoting the One Health approach.

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Structure of Bacterial Community with Resistance to Antibiotics in Aquatic Environments. A Systematic Review

Sánchez-Baena

Caicedo-Bejarano

Vivas

2021

IJERPH

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Aquatic environments have been affected by the increase in bacterial resistant to antibiotics. The aim of this review is to describe the studies carried out in relation to the bacterial population structure and antibiotic resistance genes in natural and artificial water systems. We performed a systematic review based on the PRISMA guideline (preferred reporting items for systematic reviews and meta-analyzes). Articles were collected from scientific databases between January 2010 and December 2020. Sixty-eight papers meeting the inclusion criteria, i.e., “reporting the water bacterial community composition”, “resistance to antibiotics”, and “antibiotic resistance genes (ARG)”, were evaluated according to pre-defined validity criteria. The results indicate that the predominant phyla were Firmicutes and Bacteroidetes in natural and artificial water systems. Gram-negative bacteria of the family Enterobacteraceae with resistance to antibiotics are commonly reported in drinking water and in natural water systems. The ARGs mainly reported were those that confer resistance to β-lactam antibiotics, aminoglycosides, fluoroquinolones, macrolides and tetracycline. The high influence of anthropogenic activity in the environment is evidenced. The antibiotic resistance genes that are mainly reported in the urban areas of the world are those that confer resistance to the antibiotics that are most used in clinical practice, which constitutes a problem for human and animal health.

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Antimicrobial resistance patterns and genotypic diversity between clinical and water systems isolates of Pseudomonas aeruginosa in Cali, Colombia

Cited by 3 publications

References 24 publications

Genomic Differences Associated with Resistance and Virulence in Pseudomonas aeruginosa Isolates from Clinical and Environmental Sites

Genomic Differences Associated with Resistance and Virulence in Pseudomonas aeruginosa Isolates from Clinical and Environmental Sites

Beta-Lactam-Resistant Enterobacterales Isolated from Landfill Leachates

Structure of Bacterial Community with Resistance to Antibiotics in Aquatic Environments. A Systematic Review

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