Antimicrobial resistance pattern of Pseudomonas aeruginosa isolated from various clinical samples in a tertiary care hospital, South Odisha, India

Dash, Muktikesh; Padhi, Sanghamitra; Narasimham, Moningi Venkat; Pattnaik, Swetalona

doi:10.4103/2278-0521.130200

Cited by 18 publications

(28 citation statements)

References 15 publications

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“…This result indicates high incidence of MDR-isolated clinical bacteria. Similar high rate (84%) was reported by Dash et al (23). Likewise, the findings of Okonko et al (22) who reported MDR to 5 antibiotics (ampicillin, chloramphenicol, Co-trimoxazole, nitrofurantoin, and tetracycline).…”

Section: Discussionsupporting

confidence: 90%

Surveillance of Antibiotic-Resistant Bacteria in King Khalid Hospital, Hafr Al-Batin, Saudi Arabia, During 2013

Yousef

2016

Jundishapur J Microbiol

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BackgroundResearch to understand and control the emergence and spread of antimicrobial resistance has become a public health priority.ObjectivesThis study was conducted to study epidemiology and resistant pattern of bacteria causing infection in different King Khalid hospital units.Patients and MethodsAll samples were sent to the lab and routinely processed according to the standard microbiological procedures. Then, the cultures yielding growth were selected for the study. Identification and antibiotic susceptibility test for all clinical isolates were processed by using MicroScan instrumentation. A total of 428 clinical samples were collected within 8 months; out of them, 300 clinical isolates were subjected to validation test.ResultsEscherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa were the commonly identified Gram-negative bacteria. Staphylococcus aureus was the only identified Gram-positive bacterium. The most common infections were taken from the wounds (39.0%), urinary tract (32.3%), and bloodstream (17.8%). The most common antibiotic-resistant bacteria were found on female surgical ward (100%) followed by ICU (90.2%), and male surgical ward (88.2%). The overall results of antibiotic resistance were 100% for S. aureus, 93.3% K. pneumonia, 75.7 % E. coli, and 100% for P. aeruginosa. Staphylococcus aureus showed high resistance to ampicillin and linezolid (94.1%). High (86.95%) and full resistance (100%) against ampicillin were observed from E. coli and K. pneumonia, respectively. P. aeruginosa was fully resistant to 4 antibiotics of cefazoline, cefoxitin, tetracycline, and trimethoprim-sulfamethoxazole.ConclusionsThe study was useful in determining the risk factors and defining different hospital units which should be targeted for measures to prevent infection.

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Section: Discussionsupporting

confidence: 90%

Surveillance of Antibiotic-Resistant Bacteria in King Khalid Hospital, Hafr Al-Batin, Saudi Arabia, During 2013

Yousef

2016

Jundishapur J Microbiol

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“…As shown in Table 2, imipenem was the most effective antibiotic against Pseudomonas species with 99.5% sensitivity followed by ciprofloxacin with 97.5% sensitivity. The high sensitivity of imipenem against gram-negative bacteria has been shown by others as well (Mokaddas and Sanyal, 1999;El-Tahawy, 2000;Bahashwan and Shafey, 2013;Dash et al, 2014). Ceftazine showed a sensitivity of 96.3%.…”

Section: Resultsmentioning

confidence: 87%

In vitro antibiotic resistance patterns of Pseudomonas spp. isolated from clinical samples of a hospital in Madinah, Saudi Arabia

Saeed¹,

Ghanem²,

Shafey³

et al. 2018

Afr. J. Microbiol. Res.

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Pseudomonas spp. are the leading cause of nosocomial infections. Rise in multidrug resistance among clinical isolates limit therapeutic options and hence increase mortality rate. Clinical samples (6840) from a hospital in Madinah, Saudi Arabia were collected for a duration of 14 months to study the frequency, antimicrobial sensitivity pattern and seasonal variations of Pseudomonas isolates. Conventional biochemical tests were done to identify the probable organism and antibiotic susceptibility was performed by disc diffusion method and Phoenix automated microbiology 100 ID/AST system. Pseudomonas represented 6.5% of all positive samples of which 65% were from males. Majority of the organisms (85%) were isolated from sputum and wound swabs followed by catheter tips (6.4%) and throat aspirates (3.4%). From the remaining samples, less than 1% organisms were obtained. Assessment of antimicrobial susceptibility to 11 different antibiotics revealed that imipenem was the most effective with highest sensitivity of 99.5%, and low intermediate resistance of only 0.5%. This was followed by ciprofloxacin (97.5%), ceftazidime (96.3%), cefpiramide/amikacin (94.1%), aztreonam (93.2%), gentamycin (87.7%), ampicillin (83%), and cotrimoxazole (80.1%). The most resistant drugs included augmentin (25%), cotrimoxazole (19.9%), ampicillin (17%) and gentamycin (12.3%) while the least resistant were ciprofloxacin (1.5%) and imipenem (0%). Results recommend imipenem as a promising antibiotic against Pseudomonas infections. In case of resistance to imipenem, ciprofloxacin, ceftazidime, cefpiramide, amikacin, and aztreonam may be recommended. In acute cases, Pseudomonas infections may require combined antimicrobial therapy. Frequency of these infections was the lowest (17%) during spring. It was the highest (30%) during summers and winters but reduced to 22% during autumn maybe due to better hygiene during pilgrimage season.

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“…In a study of 411 P. aeruginosa isolates in Asian hospitals, the MDR, XDR ,and PDR rates of P. aeruginosa were 42.8%, 4.9%, and 0.7%, respectively (24). In a hospital in India, 84.7% P. aeruginosa isolates were MDR, of which 35.7% were XDR, and no PDR isolate was obtained (26). Mohanasoundaram et al (27) reported a similar MDR rate (71%) in a study in Tamil Nadu, India.…”

Section: Discussionmentioning

confidence: 99%

Determination of Acquired Resistance Profiles of Pseudomonas aeruginosa Isolates and Characterization of an Effective Bacteriocin-Like Inhibitory Substance (BLIS) Against These Isolates

Shokri

Khorasgani

Zaghian

et al. 2016

Jundishapur J Microbiol

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BackgroundThe emergence of pan-drug resistant strains (PDR) of Pseudomonas aeruginosa has led to renewed efforts to identify alternative agents, such as bacteriocins and bacteriocin-like inhibitory substances (BLISs).ObjectivesThe aims of this study were to determine the acquired resistance profiles of multidrug-resistant (MDR), extensively drug-resistant (XDR), and PDR P. aeruginosa isolates based on the revised definitions of the CDC and ECDC and to screen and characterize effective BLISs against these isolates.Patients and MaterialsIn a cross-sectional study, 96 P. aeruginosa strains were isolated during a 12-month period. The resistance profiles of these isolates were determined as MDR, XDR, and PDR, and the data were analyzed using WHONET5.6 software. A BLIS against the P. aeruginosa strains was characterized based on its physicochemical properties, size, growth curves, and production profiles.ResultsAmong the 96 isolates of P. aeruginosa, 2 (2.1%), 94 (97.9%), and 63 (65.6%) were non-MDR, MDR, and XDR, respectively, and 1 (1.1%) was PDR. The most effective antibiotics against these isolates were polymyxins and fosfomycin. A BLIS isolated from the P. aeruginosa DSH22 strain had potent activity against 92 (95.8%) of the 96 isolates. The BLIS was heat stable, (up to 100°C for 10 min), UV stable, and active within a pH range of 3 - 9. The activity of BLIS disappeared when treated with trypsin, proteinase K, and pepsin, indicating its proteinous nature. Based on its size (25 kDa), the BLIS may belong to the large colicin-like bacteriocin family. BLIS production started in the midexponential phase of growth, and the maximum level (2700 AU/mL) occurred in the late-stationary phase after 25 hours of incubation at 30°C.ConclusionsThis BLIS with broad-spectrum activity may be a potential agent for the treatment or control of drug-resistant strains of P. aeruginosa infection.

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Antimicrobial resistance pattern of Pseudomonas aeruginosa isolated from various clinical samples in a tertiary care hospital, South Odisha, India

Cited by 18 publications

References 15 publications

Surveillance of Antibiotic-Resistant Bacteria in King Khalid Hospital, Hafr Al-Batin, Saudi Arabia, During 2013

Surveillance of Antibiotic-Resistant Bacteria in King Khalid Hospital, Hafr Al-Batin, Saudi Arabia, During 2013

In vitro antibiotic resistance patterns of Pseudomonas spp. isolated from clinical samples of a hospital in Madinah, Saudi Arabia

Determination of Acquired Resistance Profiles of Pseudomonas aeruginosa Isolates and Characterization of an Effective Bacteriocin-Like Inhibitory Substance (BLIS) Against These Isolates

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