Antimicrobial resistance pattern and virulence profile of S. aureus isolated from household cattle and buffalo with mastitis in Egypt

El-Ashker, Maged; Gwida, Mayada; Monecke, Stefan; El-Gohary, Fatma A.; Ehricht, Ralf; El-Sayed, M. M.; Akinduti, P. A.; El-Fateh, Mohamed; Maurischat, Sven

doi:10.1016/j.vetmic.2019.108535

Cited by 37 publications

(28 citation statements)

References 17 publications

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“…The accessory gene regulator (agr) system of S. aureus regulates numerous virulence and pathogenicity factors. CC97 MRSA strains have been repeatedly typed as agr type I in pigs and bovine isolates [17,45,46]. Nevertheless, in our study all CC97 isolates belonged to agr type III, which may be characteristic of ST2855 since in the only study reporting ST2855 in rabbits, all ST2855 isolates from farm rabbit infections were also typed as agr III [31].…”

Section: Characterization Of Cc97 Mrsa Isolatescontrasting

confidence: 53%

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Silva

Sousa

Gómez

et al. 2020

Foods

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Methicillin-resistant Staphylococcus aureus (MRSA) are one of the main pathogens associated with purulent infections. MRSA clonal complex 97 (CC97) has been identified in a wide diversity of livestock animals. Therefore, we aimed to investigate the antibiotic resistance profiles of MRSA strains isolated from purulent lesions of food-producing rabbits. Samples from purulent lesions of 66 rabbits were collected in a slaughterhouse in Portugal. Samples were seeded onto ORSAB plates with 2 mg/L of oxacillin for MRSA isolation. Susceptibility to antibiotics was tested by the disk diffusion method against 14 antimicrobial agents. The presence of resistance genes, virulence factors and the immune evasion cluster (IEC) system was studied by polymerase chain reaction. All isolates were characterized by multilocus sequence typing (MLST), agr and spa typing. From the 66 samples analyzed, 16 (24.2%) MRSA were detected. All strains were classified as multidrug-resistant as they were resistant to at least three classes of antibiotics. All isolates showed resistance to penicillin, erythromycin and clindamycin. Seven isolates were resistant to gentamicin and harbored the aac(6 )-Ie-aph (2 )-Ia gene. Resistance to tetracycline was detected in 10 isolates harboring the tet(K) gene. The IEC genes were detected in three isolates. MRSA strains belonged to CC97, CC1, CC5, CC15 or CC22. The isolates were assigned to six different spa types. In this study we found a moderate prevalence of multidrug-resistant MRSA strains in food-producing rabbits. This may represent concern for food Foods 2020, 9, 439; doi:10.3390/foods9040439 www.mdpi.com/journal/foods Foods 2020, 9, 439 2 of 10 safety and public health, since cross-contamination may occur, leading to the spread of MRSA and, eventually, the possibility of ingestion of contaminated meat.

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Section: Characterization Of Cc97 Mrsa Isolatescontrasting

confidence: 53%

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Silva

Sousa

Gómez

et al. 2020

Foods

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“…Yang et al (2020) found high frequencies of SE genes in MRSA isolates of bovine mastitis cases from China. SE genes were also detected in MRSA strains of raw milk from Italy (Riva et al, 2015) and Egyptian dairy herds (El-Ashker et al, 2020). In contrast, Kadlec et al (2019) and Kreausukon et al (2012) did not detect any SE genes in LA-MRSA strains from German dairy farms.…”

Section: Low Risk For Public Healthmentioning

confidence: 83%

Genomic Distinctions of LA-MRSA ST398 on Dairy Farms From Different German Federal States With a Low Risk of Severe Human Infections

et al. 2021

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Methicillin-resistant Staphylococcus aureus (MRSA) have been found on German dairy farms and may be the cause of difficult-to-treat bovine mastitis. Considering the one health approach, MRSA might be transmitted from animals to humans raising the risk for severe infections. On 17 German dairy farms with a history of MRSA detection, MRSA strains were isolated from quarter milk, bulk tank milk, and swab samples of calves, heifers, pigs, and the environment. A selection of 33 isolates was analyzed using whole-genome sequencing and antimicrobial resistance testing. All detected MRSA strains were attributed to the livestock-associated sequence type 398. Methicillin-resistance was associated with the mecA gene in the staphylococcal cassette chromosome (SCC)mec types IVa (7/33) or V (26/33). The MRSA strains across the German federal states showed large allelic differences indicating independent development and distribution. On one farm, a clonal MRSA isolate was widely spread among different animals and the milking equipment. Moreover, MRSA transmission between two dairy farms in one federal state seems to be likely. In depth studies indicated that the resistance gene prediction and phenotypic resistance are in good agreement. Twenty eight strains were determined to exhibit a non-wildtype phenotype (resistant) against up to seven antimicrobial substances with an overall resistance to β-lactams and tetracycline. Ten different phenotypic antimicrobial resistance patterns were found among the MRSA strains. The strains harbored a wide virulence gene repertoire, of which some of them are related to bovine mastitis. However, the isolates lacked typical human infection associated factors such as the immune evasion cluster genes, staphylococcal enterotoxin genes, or Panton-Valentine leukocidin genes leading to the assumption for a low risk for severe human infections and foodborne diseases.

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“…A utilização de extratos vegetais com ação antimicrobiana foi demonstrada com eficiência em testes in vitro e in vivo na anti-sepsia de tetos antes e após a ordenha, embasando estudos que visam a promoção à integridade do animal e da qualidade do leite a partir de antimicrobianos alternativos (SCHUCH et al, 2008;AVANCINI;FACCIN et al, 2016). Devido as suas características na medicina popular mexicana, Jatropha spp.…”

Section: Resultsunclassified

Qualidade Microbiologica Do Leite: Jatropha Dioica Contra Bacterias De Mastite Bovina

Silva¹,

Labrecht²,

Carla³

et al. 2020

Ciência, Tecnologia E Inovação: Do Campo À Mesa

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Antimicrobial resistance pattern and virulence profile of S. aureus isolated from household cattle and buffalo with mastitis in Egypt

Cited by 37 publications

References 17 publications

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Genomic Distinctions of LA-MRSA ST398 on Dairy Farms From Different German Federal States With a Low Risk of Severe Human Infections

Qualidade Microbiologica Do Leite: Jatropha Dioica Contra Bacterias De Mastite Bovina

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