Antimicrobial Resistance of Listeria monocytogenes Strains Isolated from Humans, Animals, and Food Products in Russia in 1950–1980, 2000–2005, and 2018–2021

Abstract

Susceptibility of 117 L. monocytogenes strains isolated during three time periods (1950–1980; 2000–2005, and 2018–2021) to 23 antibiotics was tested by the disk diffusion method. All strains were sensitive to aminoglycosides (gentamicin, kanamycin, neomycin, streptomycin), glycopeptides (vancomycin and teicoplanin), clarithromycin, levofloxacin, amoxicillin/clavulanic acid, and trimethoprim/sulfamethoxazole. Resistance to clindamycin was observed in 35.5% of strains. Resistance to carbapenems, imipenem and meropenem was found in 4% and 5% of strains, respectively. Resistance to erythromycin, penicillin G, trimethoprim, and ciprofloxacin was found in 4%, 3%, 3%, and 2.5% of strains, respectively. Resistance to tylosin, ampicillin, enrofloxacin, linezolid, chloramphenicol, and tetracycline was found in less than 2%. Three strains with multiple antibiotic resistance and 12 strains with resistance to two antibiotics were revealed. Comparison of strains isolated in different time periods showed that the percentage of resistant strains was the lowest among strains isolated before 1980, and no strains with multiple antibiotic resistance were found among them. Statistical analysis demonstrated that the temporal evolution of resistance in L. monocytogenes has an antibiotic-specific character. While resistance to some antibiotics such as ampicillin and penicillin G has gradually decreased in the population, resistance to other antibiotics acquired by particular strains in recent years has not been accompanied by changes in resistance of other strains.

Prevalence and Antimicrobial Resistance of Virulent Listeria monocytogenes and Cronobacter sakazakii in Dairy Cattle, the Environment, and Dried Milk with the In Vitro Application of Natural Alternative Control

“…In another study [ 34 ], all the isolates of the Listeria species were observed to be resistant against penicillin and imipenem (100%), followed by trimethoprim (75%). Another study [ 35 ] showed resistance to carbapenems, imipenem, and meropenem in 4% and 5% of strains collected from humans, animals, and food products in Russia, respectively. On other hand, 100% resistance to imipenem was recorded in [ 34 ]; however, gentamicin and meropenem appeared to be the most effective antibiotics, as all Listeria species isolates were susceptible to them.…”

“…Vancomycin susceptibility (97.9%) was the highest among all tested antibiotics, as recorded in [ 2 ], which found that all L. monocytogenes strains (100) were susceptible to vancomycin. In contrast, all L. monocytogenes strains were resistant to vancomycin in [ 35 ]. Our findings reveal that 100% of the examined L. monocytogenes had MAR ranging from 0.33 to 0.88, which matches the result of Elsayed et al [ 2 ].…”

Distribution of antimicrobial resistance among clinical and food Listeria monocytogenes isolated in Moscow in 2019–2021

“…№2 АНТИБИОТИКОРЕЗИСТЕНТНОСТЬ ным значением между устойчивыми и чувствительными штаммами L. mono cytogenes [23]; для 8 штаммов размер зоны подавления роста составил 17 мм (Рисунок 4). Для более наглядного представления результатов мы срав нили размеры зон подавления роста к ампициллину и бензилпенициллину у исследованных штаммов и у штам мов, выделенных на территории России в 1950-1980 гг., чувствительность которых была определена ранее с ис пользованием дисков того же производителя (Рисунок 4) [14]. Аналогичный сдвиг в размере зон подавления ро ста наблюдали и в устойчивости к бензилпенициллину.…”

“…Том 24 . №2 АНТИБИОТИКОРЕЗИСТЕНТНОСТЬ числе множественной, устойчивости к антибиотикам, особенно среди клинических изолятов и изолятов, выде ленных из окружающей среды [14][15][16].…”

Synthetic oxepanoprolinamide iboxamycin is highly active against human pathogen Listeria monocytogenes

“…Antibiotic treatment options for L. monocytogenes infections include cell wall synthesis disruptors ampicillin and vancomycin, folic acid synthesis inhibitors sulfamethoxazole and trimethoprim, and protein synthesis inhibitors, such as gentamicin and azithromycin [33]. L. monocytogenes strains reported in recent years are often resistant to clindamycin, with the resistant fraction ranging from 29% to 76%, depending on the collection [34][35][36][37], thus excluding clindamycin as a viable option for treatment of L. monocytogenes infections. Importantly, just as E. faecalis encodes the ABCF ATPase LsaA, L. monocytogenes encodes the ARE ABCF PLSA resistance factor VgaL/Lmo0919 in its core genome [38].…”