Antimicrobial resistance in Escherichia coli isolates from food animals, animal food products and companion animals in China

Lei, Tao; Tian, Wei; Liu, He; Huang, Xianhui; Sun, Yanwu; Deng, Yuting; Sun, Yi-Ming; Lv, Dian-Hong; Wu, Congming; Huang, Li; Shen, Jianzhong; Liu, Jianhua

doi:10.1016/j.vetmic.2010.04.025

Cited by 76 publications

(62 citation statements)

References 19 publications

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“…These two molecules (streptomycin and tetracycline) are used very frequently and for a long time in the poultry sector. Studies made by other authors [14,19] were in agreement with our fi ndings. Indeed, they showed a high rates of resistance to tetracycline (90.8-95.2 %), and streptomycin (46 %).…”

Section: Discussionsupporting

confidence: 93%

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Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Abbassi¹

2017

J Clin Microbiol Biochem Technol

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A total of 174 E. coli isolates collected from healthy poultry, bovine and ovine recovered between December 2009 and June 2013 in different geographic location in Tunisia, were assessed and examinated for resistance to antimicrobial agents. Avian isolates showed the highest rates of antibiotic resistance: tetracycline (74.7 %), trimethoprim/sulfamethoxazole and amoxicillin with the same rate of resistance (57 %). Prevalences of resistance to the same four antimicrobials in bovine isolates were 33.3 %, 65 %, 30 %, 28.3 %, respectively. However, for ovine isolates, low resistance rates were observed, except for tetracycline (40 %) and amoxicillin (22.85 %). Only one ESBL-producing isolate from chicken was detected. In addition, seventy-seven (44.2 %) isolates were resistant to three or more classes of antibiotics and were considered multidrug resistant (MDR). Interestingly, avian E. coli isolates were more resistant than bovine and ovine ones. These results provide novel insights into the epidemiological characteristics of poultry, bovine and ovine E.coli isolates in Tunisia, and suggest the need for the prudent use of antimicrobial agents in husbandry and the urgent need to establish a national antibiotic resistance monitoring program.

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Section: Discussionsupporting

confidence: 93%

“…were observed worldwide [14,19,37]. For ovine isolates, the frequencies of resistance were low and almost all isolates were susceptible to the tested antibiotics, except for tetracycline (40 %) and to amoxicillin (22,85 %).…”

Section: Discussionmentioning

confidence: 98%

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Abbassi¹

2017

J Clin Microbiol Biochem Technol

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“…In our study, the presence of class 1 integrons was demonstrated in 18 isolates, while class 2 integrons were detected in only 1 strain. These results are consistent with other studies that showed the dominance of class 1 integrons over class 2 integrons in E. coli of human and animal origin [20,21]. The class 1 integrons were functional and capable of integrating multiple genes cassettes in their variable regions, including their expression, and consequently by providing a common promoter [26].…”

Section: Discussionsupporting

confidence: 92%

“…The results also showed low levels of resistance to amoxicillin/clavulanic acid, ceftazidime, and cefotaxime. Similar results have been reported in E. coli strains isolated from animal origins, especially avian isolates, in many countries including Tunisia [20,21]. High rates of antimicrobial resistance in E. coli have been reported in Tunisian patients [22][23][24].…”

Section: Discussionsupporting

confidence: 83%

Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia

Kilani

Abbassi

Ferjani

et al. 2017

J Infect Dev Ctries

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Introduction: The virulent Escherichia coli strains responsible for extraintestinal infections were mainly belonged to B2 and D phylogroups. However, no past studies have determinate via the presence of virulence genes the frequency of E. coli pathovars recovered from animals housed in farms in Tunisia. The aims of this study were to investigate 26 E. coli isolated from healthy and diarrheic animals and to determinate via the presence of virulence genes the frequency of pathovars. Methodology: Twenty-six E. coli isolates of phylogroups B2 (n = 14), B22 (n = 9), B23 (n = 5), and D2 (n = 12) were characterized. Genes encoding virulence factors (fimH,eaeA,aggC,papC, papG allele III, hlyA, east1, cnf1, exhA,stx1, stx2, iutA, fyuA, ibeA,and ipaH), and antibiotic resistance as well as class 1 and 2 integrons were searched by polymerase chain reaction (PCR). The genetic relationship of isolates was done by PFGE. Results: According to the occurrence of specific genes the 26 isolates were classified as:9 EAEC, 2 EHEC, 4 UPEC, 3 EPEC/EHEC and 1 NTEC. Therefore, 2 Ex-PEC and 5 APEC were presented amongst our strains. Some isolates (12) were clonal and the remaining was unrelated. Conclusions: Higher diversity of pathovars which carried diverse combinations of virulence genes in healthy isolates. In addition, it seems that the infections were caused by different mechanisms.

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“…The present study explored virulence gene frequencies and the association between virulence gene profiles, phylogenetic group, ST type, and fluoroquinolone resistance among E. coli from dogs and cats in China. Our study revealed high resistance to tetracycline, ampicillin, amoxicillin/clavulanic acid, trimethoprimsulfamethoxazole, gentamicin, and enrofloxacin, and showed a similar pattern found in another study among E. coli isolates from companion animals in China [20]. The resistance rate to fluoroquinolones (60.9%) was similar to that found in previous studies among foodproducing animals and humans in China [21,22], while it was significantly greater than the fluoroquinolone resistance rates (about 10%) in companion animals in the United States and Portugal [23,24].…”

Section: Discussionsupporting

confidence: 88%

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Liu

et al. 2017

J Infect Dev Ctries

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Introduction: Escherichia coli is not only a commensal organism in humans and animals, but also a causative agent of diarrhea and extraintestinal infections. Information about the relationship between population structure, virulence gene profiles, and fluoroquinolone resistance of E. coli in dogs and cats in China is limited. Methodology: A total of 174 pathogenic and commensal E. coli isolates were evaluated in terms of phylogenetic group, virulence gene profile, sequence types (STs), and fluoroquinolone susceptibility. Results: A total of 46.6% of isolates belonged to phylogenetic group B2. Isolates displayed high resistance to tetracycline (82.2%), amoxicillin/clavulanic acid (73.6%), gentamicin (62.1%), and enrofloxacin (60.9%). fimH (81.6%) was the most prevalent virulence gene, and 83.9% of isolates contained one or more investigated virulence genes. The majority of the investigated virulence genes were more prevalent in fluoroquinolone-susceptible isolates and pathogenic isolates. Multilocus sequence typing (MLST) showed that E. coli isolates analyzed were assigned to 65 STs. Among of them, pathogenic-resistant and pathogenic-susceptible isolates had 44 and 10 STs, respectively, while there were 8 and 3 STs in the commensal resistant and susceptible isolates, respectively. Conclusions: Phylogenetic group B2 was the dominant group, accounting for 46.6% of the isolates. Pathogenic isolates and fluoroquinolonesusceptible isolates possessed more virulence genes. Pathogenic isolates and fluoroquinolone-resistant isolates exhibited high population diversity, and pandemic clone ST131 appeared in 9.8% of isolates.

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Antimicrobial resistance in Escherichia coli isolates from food animals, animal food products and companion animals in China

Cited by 76 publications

References 19 publications

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

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