Antimicrobial Resistance in Bacterial Pathogens of Canine Otitis

Abstract

Abstract:Otitis is one of the most frequent infections in dogs. This is attributed to the misuse of drugs enabling generation of multi-resistant micro-organisms. The emergence of multiresistant bacterial strains in veterinary medicine is a reality that must be studied and evaluated by the professionals. The objective of this study was to isolate and evaluate the antimicrobial susceptibility of bacterial pathogens of otitis in dogs. Otologic swabs were collected from 36 dogs with clinical otitis. 41 bacterial strains were isolated and antimicrobial susceptibility tests were performed by disk diffusion method with 34 antimicrobial agents. Presence of the resistance gene mecA of Staphylococcus was examined for 22 strains of staphylococci by PCR. A total of 1108 ratings antimicrobial agents were performed. The percentage of drug resistance was 34.66% (n = 384) of the assessments with partial or total resistance. Major bacterial pathogens were Staphylococcus spp. (65.85%), Pseudomonas spp. (12.19%) and Enterobacteria species (19.51%). 53.66% of the isolates were considered multiresistant. Antimicrobial agents considered most resistant in the strains studied were penicillin (75.00%), tetracyclin (50.00%), amoxicillin (48.78%), trimethoprimsulfamethoxazole (46.15%), clindamycin and rifampicin (43.24%). 11 strains were phenotypically characterized as MRS, 4 genotypically as MRS, 2 as MLSB-MRS and 2 as gram negative ESBL-producing.

Isolation of multidrug-resistant Escherichia coli, Staphylococcus spp., and Streptococcus spp. from dogs in Chattogram Metropolitan Area, Bangladesh

“…isolates, high resistance was found toward penicillin (63%) and ceftriaxone (54%), which was higher than that in the study conducted by Norton et al [ 31 ]. The resistance to ampicillin, erythromycin, and azithromycin was 38%, 29%, and 4%, respectively, which is lower than the results obtained in an earlier study [ 32 ]. In this study, we found that Streptococcus spp.…”

Susceptibilidad a antibióticos en cepas de Pseudomonas aeruginosa aisladas de canes con otitis externa

“…Considerando las clases de antibióticos, los mayores porcentajes de resistencia se observaron en el grupo de las sulfamidas 80% (16/20), seguido de los betalactámicos 75% (30/40) y las lincosaminas 75% (6/8). Este hecho concuerda con datos obtenidos por otros investigadores 2,15,26 , quienes en cada clase casi siempre probaron un número diferente de antibióticos, los cuales también podían pertenecer a diferentes grupos dentro de una misma clase.…”

“…En cuanto a la multirresistencia, el 70% de las cepas (14/20) presentaron esta característica, notándose que entre ellas, 10 cepas mostraron resistencia a tres clases de antibióticos, 2 frente a cuatro clases y otras 2 frente a cinco clases. Porcentajes menores han sido reportados por otros autores 7,26 .…”

Multidrug-Resistant Bacterial Pathogens Assessment in Canine Ophthalmic Infections

“…The PCR was performed according to Sfaciotte et al (2015a) with primers SMAswF (5'-GAT GAT ACC TTC GTT CCA C-3' nt 622-640) and SMAswR (5'GTA TGT GCG ATT GTA TTG C-3' nt 917-935) that amplify a 314 bp.…”