Antimicrobial resistance genes, virulence markers and prophage sequences in Salmonella enterica serovar Enteritidis isolated in Tunisia using whole genome sequencing

Ksibi, Boutheina; Ktari, Sonia; Ghedira, Kaïs; Othman, Houcemeddine; Maâlej, Sami; Mnif, Basma; Fabre, Laëtitia; Rhimi, Faouzia; Hello, Simon Le; Hammami, Adnène

doi:10.1016/j.crmicr.2022.100151

Cited by 4 publications

(5 citation statements)

References 38 publications

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“…These invasion proteins, such as invasins and intimin, manipulate the host cell’s signaling pathways, enabling the bacteria to enter and replicate within the host cells. Virulence genes targeted by PCR include invA [ 107 ], a component of the Salmonella pathogenicity island 1, which is crucial for efficient cell invasion [ 108 , 109 ]. fimA can also be researched, as it is necessary for the assembly of type 1 fimbriae [ 110 ], and the enterotoxin gene from S. typhimurium ( stn ) can also be targeted [ 111 ].…”

Section: How To Translate Microbial Analysis In Term Of Public Health...mentioning

confidence: 99%

A Comprehensive Review for the Surveillance of Human Pathogenic Microorganisms in Shellfish

Desdouits,

Reynaud,

Philippe

et al. 2023

Microorganisms

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Bivalve molluscan shellfish have been consumed for centuries. Being filter feeders, they may bioaccumulate some microorganisms present in coastal water, either naturally or through the discharge of human or animal sewage. Despite regulations set up to avoid microbiological contamination in shellfish, human outbreaks still occur. After providing an overview showing their implication in disease, this review aims to highlight the diversity of the bacteria or enteric viruses detected in shellfish species, including emerging pathogens. After a critical discussion of the available methods and their limitations, we address the interest of technological developments using genomics to anticipate the emergence of pathogens. In the coming years, further research needs to be performed and methods need to be developed in order to design the future of surveillance and to help risk assessment studies, with the ultimate objective of protecting consumers and enhancing the microbial safety of bivalve molluscan shellfish as a healthy food.

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Section: How To Translate Microbial Analysis In Term Of Public Health...mentioning

confidence: 99%

A Comprehensive Review for the Surveillance of Human Pathogenic Microorganisms in Shellfish

Desdouits,

Reynaud,

Philippe

et al. 2023

Microorganisms

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“…Suez et al (2013) reported the universal presence of SPIs 1-5, 9, 13, and 14, the absence of SPIs 7, 8 and 15, and the variable or mosaic presence of SPIs 6, 10-12 and 16-19 in the genome of all non-typhoidal Salmonella isolates. Moreover, an analysis of 45 strains of Salmonella Enteritidis demonstrated that six SPIs, including SPI-1, SPI-2, SPI-3, SPI-9, SPI-13, and CS54, are conserved in all isolates while SPI-11 and SPI-12 were absent (Ksibi et al, 2022).…”

Section: B C 4 Discussionmentioning

confidence: 99%

“…The intact regions correspond to Salmon_118970_sal3 and Gifsy-2. These are common regions for the serotype Enteritidis (Ksibi et al, 2022). Gifsy-2 is a lambdarelated prophage that may play an important role in resistance to oxidative stress in Salmonella Enteritidis PT4 578 since it it harboredsuperoxide dismutase sodCI.…”

Section: B C 4 Discussionmentioning

confidence: 99%

“…Quorum sensing generalist strains, whereas most host-adapted strains cannot form biofilms . Genetic comparisons between serotypes demonstrated that fimbrial operons, especially the csg operon, are conserved in the genus Salmonella (Suez et al, 2013;Ksibi et al, 2022).…”

Section: Motilitymentioning

confidence: 99%

“…Rev. 44,[106][107][108][109][110][111][112][113][114][115][116][117][118][119][120][121][122].1093/femsre/fuz029 Ksibi, B., Ktari, S., Ghedira, K., Othman, H., Maalej, S., Mnif, B., et al (2022)…”

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Whole genome sequencing analysis and biofilm formation in Salmonella Enteritidis PT4 578

Carneiro

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Salmonella is an important and versatile foodborne pathogen responsible for numerous infections and deaths worldwide. The difficulty of controlling and eradicating this pathogen is related to its ability to form complex aggregates of cells, called biofilms. In a biofilm, cells are surrounded by a matrix of self-produced extracellular polymeric substances that protect against various types of stress, such as desiccation, sanitization, and host defense. The Salmonella biofilm matrix is composed mainly of curli fimbriae and cellulose. A highly complex genetic network regulates the synthesis of these components. The main regulators associated with biofilm formation are the CsgD protein and the bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP), which activate the biosynthesis of most polymers. Quorum sensing, a cellular communication mechanism, is also described as an important regulator of biofilm formation in Salmonella. The objective of the present work was to sequence the genome of Salmonella enterica Enteritidis PT4 578, analyze the main characteristics related to virulence and biofilm formation, and compare the genome and morphotype formation, biofilm and motility phenotypes with four related serotypes. Furthermore, evaluate the influence of temperature, atmosphere, and quorum sensing on biofilm formation by this serotype. Genome analyzes showed that Salmonella Enteritidis PT4 578 has 165 virulence genes, representing 3.66% of the coding sequences. Twelve Salmonella pathogenicity islands (SPI) were identified, some extremely conserved, such as SPI1 and SPI2, that encode the apparatus of intracellular invasion and colonization,. A total of thirteen gene clusters related to fimbriae biosynthesis, including the csg operon, were also annotated. Under aerobic and anaerobic conditions, Salmonella Enteritidis PT4 578 can form a biofilm on stainless steel coupons at 28 °C and only under aerobic conditions at 37 °C. However, adding of N-hexanoyl homoserine lactone (C12-HSL) to the anaerobic culture medium reverses the biofilm formation phenotype at 37 °C. In this condition, C12-HSL increased the expression of the adrA gene, a diguanylate cyclase related to the synthesis of c-di-GMP and the luxS gene, which is part of another quorum sensing mechanism that uses autoinducer-2 (AI-2), present in Salmonella. Although it did not influence biofilm formation at 28 ºC, C12- HSL also increased luxS expression. The data presented here reinforce the role of conditions of atmosphere and temperature, as well as quorum sensing in Salmonella biofilm formation. Finally, it reports a possible connection between this pathogen's two main quorum sensing mechanisms. Understanding the factors that influence Salmonella biofilm formation can contribute to developing control and eradication strategies. Keywords: Genome analyses. Biofilm. Quorum sensing.

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Probiotic and Safety Evaluation of Twelve Lactic Acid Bacteria as Future Probiotics

Hu,

Xie,

et al. 2023

Foodborne Pathogens and Disease

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Antimicrobial resistance genes, virulence markers and prophage sequences in Salmonella enterica serovar Enteritidis isolated in Tunisia using whole genome sequencing

Cited by 4 publications

References 38 publications

A Comprehensive Review for the Surveillance of Human Pathogenic Microorganisms in Shellfish

A Comprehensive Review for the Surveillance of Human Pathogenic Microorganisms in Shellfish

Whole genome sequencing analysis and biofilm formation in Salmonella Enteritidis PT4 578

Probiotic and Safety Evaluation of Twelve Lactic Acid Bacteria as Future Probiotics

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