Antimicrobial resistance and integron gene cassette arrays in commensal Escherichia coli from human and animal sources in IRI

Kheiri, Roohollah; Akhtari, Leili

doi:10.1186/s13099-016-0123-3

Cited by 51 publications

(35 citation statements)

References 40 publications

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“…In this study, we investigated the occurrence and cassette region composition of Class 1 integrons among 50 E. coli isolates from minks. Among the 50 isolates recovered from minks, 7 isolates (7/50, 14.0 %) carried Class 1 integrons, which was similar to that (16.0 %) of a previous study in sheep [6] but was lower than that of rabbits and cattle [45,46]. These different results may be due to differences in species, environments and regions.…”

Section: Biomed Research Internationalsupporting

confidence: 80%

“…Antibiotics have long been considered as the first line of defense to prevent pathogenic E. coli infections. The treatment of pathogenic E. coli infections is becoming complicated because of the rapid emergence and dissemination of antibiotic-resistant strains, which may consequently result in an increasing number of clinical treatment failures in bacterial-mediated diseases and further threaten public health with the possibility of transmission to humans through aerosols, environmental contact or other methods [2,6]. The level of antibiotic resistance among pathogenic and commensal E. coli has steadily increased and has become a global health concern [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Molecular and Phenotypic Characteristics ofEscherichia coliIsolates from Farmed Minks in Zhucheng, China

Qiu

Jiang

Zhang

et al. 2019

BioMed Research International

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In this study, the prevalence, phenotypes, and clonal relationships of Escherichia coli (E. coli) strains isolated from minks were investigated. In July 2017, a total of 62 fresh faecal swab samples were randomly collected from one large-scale mink farm in Zhucheng, Shandong Province, China. In all the samples, 50 E. coli strains were isolated and then assigned to serotyping, antimicrobial susceptibility test, detection of antimicrobial resistance genes and the Class 1 integrons, and multilocus sequence typing (MLST). Four pathogenic serotypes were identified among all the isolates, while the most common serotype was enterohemorrhagic E. coli O104:H4 (6.0 %). Antimicrobial sensitivity testing revealed that most isolates were susceptible to cefoxitin (96.0 %) and amikacin (82.0 %), while most isolates were resistant to ampicillin (92.0 %) and tetracycline (90.0 %). An analysis of the nucleotide sequences revealed that 7 isolates (14.0%) carried 4 types of Class 1 integron cassette, including dfrA27+aadA2+qnrA (57.1%), dfrA17+aadA5 (14.3%), dfrA12+aadA2 (14.3%), and dfrA1+aadA1 (14.3%). PCR screening showed that 14 antibiotic resistance genes were presented in 50 isolates, while the most prevalent resistance gene was qnrS, which was detected in 60.0 % of isolates, followed by sul2 (40.0%) and oqxA (38.0%). MLST analysis showed that 32 sequence types (STs) were identified, while ST46 was the predominant genotype among all isolates. Clonal complex 3 (CC3) was dominant. Compared with 340 human E. coli STs reported in China, the ST10 clonal complex, known as the largest human clonal complex, was also found in the 50 mink E. coli isolates. Meanwhile, mink-derived strain ST206 formed a new clonal complex, CC206, which was different from human ST strains. Our results showed that farmed minks could be reservoirs of antimicrobial-resistant E. coli with Class 1 integron cassettes and resistance genes, which were likely to pose a threat to public health. Therefore, continuous inspections and monitoring of E. coli in minks are essential for detecting and controlling emerging E. coli with different serovars as well as antibiotic resistance.

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Section: Biomed Research Internationalsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Molecular and Phenotypic Characteristics ofEscherichia coliIsolates from Farmed Minks in Zhucheng, China

Qiu

Jiang

Zhang

et al. 2019

BioMed Research International

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“…The dfrA1 and dfrA17 alleles were detected in 11.1 and 61.1 % of UPEC isolates, respectively. The two different dfr alleles detected in this population are among the most widespread worldwide, together with other dfr alleles (dfrA5, dfrA7 and dfrA12) [14,[30][31][32]. The high prevalence of dfr observed in this study may be related to the selective pressure exerted by trimethoprim-sulfamethoxazole, which is often used to treat urinary tract infections.…”

Section: Molecular Characterization Of Gene Cassettesmentioning

confidence: 55%

Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

Oliveira-Pinto

Diamantino

Oliveira

et al. 2017

Journal of Medical Microbiology

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Purpose. Class 1 integrons are among the main vehicles that facilitate the spread of antibiotic-resistance genes, with serious public health consequences. The aim of this cross-sectional study was to investigate the presence of class 1 integrons and to characterize their variable regions, as well as the antimicrobial resistance profiles and phylogenetic groups of a collection of Escherichia coli isolates recovered from healthy subjects (n=42) and those with urinary infection (n=40).Methodology. The methods used included PCR, sequencing and antimicrobial susceptibility testing.Results. PCR screening for the integrase gene (intI1) revealed a higher incidence of class 1 integrons in uropathogenic E. coli (65 %, UPEC) than in commensal isolates (11.9 %). Eight of 31 intI1-positive isolates, all of them UPEC, harboured empty integrons. The variable regions of the other 23 contained gene cassettes encoding resistance to b-lactams (blaOXA-1), aminoglycosides (aadA1 and aadA5), trimethoprim (dfrA1 and dfrA17) and an ORF. To our knowledge this is the first report of an ORF identified as a putative phage tail protein associated with a class 1 integron. The aadA1 and dfrA17-addA5 arrays prevailed in commensal E. coli and UPEC, respectively. UPEC isolates were highly resistant to the antimicrobials tested, in contrast to commensal isolates. The E. coli isolates carrying gene cassettes associated with class 1 integrons were found to be unrelated to any phylogroup or multiresistance.Conclusion. Co-resistance to clinically relevant fluoroquinolone and trimethoprim-sulfamethazole in all UPEC isolates is a cause for concern. These results expand the current knowledge of gene cassettes in both commensal and pathogenic E. coli.

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“…However, the lack of gene cassettes in this study could be due to the absence of resistance genes in gene cassette arrays or lack of 3′ conserved region or absence of primer hybridization site or occurrence of early stop codon (Barlow et al . ; Kheiri and Akhtari ).…”

Section: Resultsmentioning

confidence: 98%

Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug-resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata)

Hossain

Silva

Wickramanayake

et al. 2019

Letters in Applied Microbiology

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Aeromonas sp. are opportunistic pathogenic bacteria which are associated with various diseases in ornamental fish, aquaculture raised species and wild fisheries. In our study, antimicrobial resistance patterns, antimicrobial resistance genes and class 1 integron gene cassettes of 52 guppy‐borne Aeromonas sp. were examined. The isolates were identified as A. veronii (n = 34), A. dhakensis (n = 10), A. hydrophila (n = 3), A. caviae (n = 3) and A. enteropelogenes (n = 2) by gyrB gene sequencing. Every isolate was resistant to at least four antimicrobials in disc diffusion test. The resistance to amoxicillin, nalidixic acid and oxytetracycline was 100% among the tested isolates. 92·30, 76·92, 71·15, 51·92, 51·92 and 50·00% of the isolates were resistant to ampicillin, rifampicin, imipenem, cephalothin, tetracycline and trimethoprim respectively. The multiple antibiotic resistance index values ranged from 0·28 to 0·67. PCR amplification of antimicrobial resistance genes implied the occurrence of tetracycline resistance (tetA (65·39%), tetE (25·00%) and tetB (15·38%)), plasmid‐mediated quinolone resistance (qnrS (26·92%) and qnrB (17·31%)) and aminoglycoside resistance (aphaAI‐IAB (7·69%) and aac (6′)‐Ib (3·84%)) genes in the isolates. The IntI gene was positive for 36·54% of the isolates and four class 1 integron gene cassette profiles (aadA2, qacE2‐orfD, aadA2‐catB2 and dfrA12‐aadA2) were identified. These data suggest that ornamental guppy can be a reservoir of multidrug‐resistant Aeromonas sp. which comprise different antimicrobial resistance genes and class 1 integrons. Significance and Impact of the Study Antimicrobial resistance genes and integron gene cassettes of ornamental fish‐borne aeromonads are poorly studied. The antimicrobial resistance patterns, antimicrobial resistance genes and class 1 integron gene cassettes of Aeromonas sp. isolated from ornamental guppy were characterized for the first time in Korea. The incidence of different antimicrobial resistance genes and class 1 integron gene cassettes were observed in multidrug‐resistant Aeromonas isolates. This result suggests that better management practices are necessary to prevent and address the serious consequences of indiscriminate and inappropriate antimicrobial use, and the distribution of multidrug‐resistant bacteria.

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Antimicrobial resistance and integron gene cassette arrays in commensal Escherichia coli from human and animal sources in IRI

Cited by 51 publications

References 40 publications

Molecular and Phenotypic Characteristics ofEscherichia coliIsolates from Farmed Minks in Zhucheng, China

Molecular and Phenotypic Characteristics ofEscherichia coliIsolates from Farmed Minks in Zhucheng, China

Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug-resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata)

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