Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective

Silva, Vanessa; Correia, Susana; Rocha, Jaqueline; Manaia, Célia M.; Silva, Adriana; García‐Díez, Juan; Pereira, José Eduardo López; Semedo-Lemsaddek, Teresa; Igrejas, Gilberto; Poeta, Patrícia

doi:10.3390/microorganisms10050941

Cited by 8 publications

(12 citation statements)

References 85 publications

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“…In three of our previous studies, the frequency of S. aureus and MRSA was investigated in healthy humans without recent contact with the hospital environment but with direct contact with livestock and pets, namely, cows, donkeys and dogs. Humans handling cows and donkeys were not colonized by MRSA but were S. aureus carriers [ 70 , 71 ]. However, several different clonal lineages were detected among humans, including ST398 and ST97 (animal-associated) and ST30 and ST8 (community-associated).…”

Section: Mrsa In Portugalmentioning

confidence: 99%

“…Nevertheless, other Portuguese studies have reported that 13.1% of the cows screened were colonized by S. aureus. The most common S. aureus lineage found was ST6-t16615 and most isolates were susceptible to all antimicrobials tested or showed resistance to penicillin [ 71 ]. In a study carried out by our research team, 30% of the healthy quails screened carried MRSA and only STs (ST398 and ST6831) and spa -types (t011 and t9747) were detected [ 37 ].…”

Section: Mrsa In Portugalmentioning

confidence: 99%

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MRSA in Humans, Pets and Livestock in Portugal: Where We Came from and Where We Are Going

et al. 2022

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Over the years, molecular typing of methicillin-resistant S. aureus (MRSA) has allowed for the identification of endemic MRSA strains and pathogenic strains. After reaching a peak of predominance in a given geographic region, MRSA strains are usually replaced by a new strain. This process is called clonal replacement and is observed worldwide. The worldwide spread of hospital-associated MRSA (HA-MRSA), community-associated MRSA (CA-MRSA) and livestock-associated MRSA (LA-MRSA) clones over the last few decades has allowed this microorganism to be currently considered a pandemic. In Portugal, most HA-MRSA infections are associated with EMRSA-15 (S22-IV), New York/Japan (ST5-II) and Iberian (ST247-I) clones. Regarding the strains found in the community, many of them are frequently associated with the hospital environment, namely the Pediatric, Brazilian and Iberian clones. On the other hand, a strain that is typically found in animals, MRSA clonal complex (CC) 398, has been described in humans as colonizing and causing infections. The ST398 clone is found across all animal species, particularly in farm animals where the economic impact of LA-MRSA infections can have disastrous consequences for industries. In contrast, the EMRSA-15 clone seems to be more related to companion animals. The objective of this review is to better understand the MRSA epidemiology because it is, undoubtedly, an important public health concern that requires more attention, in order to achieve an effective response in all sectors.

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Section: Mrsa In Portugalmentioning

confidence: 99%

Section: Mrsa In Portugalmentioning

confidence: 99%

MRSA in Humans, Pets and Livestock in Portugal: Where We Came from and Where We Are Going

et al. 2022

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“…The usage of antibiotics creates selective pressure, driving antimicrobial resistance (AMR) in both human and veterinary medicine [ 14 , 15 ]. The spread of AMR S. aureus strains between livestock and humans underscores the need for a One Health approach, with several studies indicating the zoonotic potential of certain S. aureus lineages [ 4 , 16 , 17 ]. Since cattle domestication, the proximity of humans and cows or the consumption of raw milk and dairy products have increased the risk of S. aureus spillovers [ 16 , 18 – 20 ].…”

Section: Introductionmentioning

confidence: 99%

An observational study demonstrates human-adapted Staphylococcus aureus strains have a higher frequency of antibiotic resistance compared to cattle-adapted strains isolated from dairy farms making farmstead cheese

Chakrawarti,

Casey,

Burk

et al. 2024

BMC Vet Res

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Background Staphylococcus aureus is a multi-host zoonotic pathogen causing human and livestock diseases. Dairy farms that make artisan cheese have distinctive concerns for S. aureus control. Antimicrobial-resistant (AMR) S. aureus is a public and animal health concern. There is a need to study the population structure of AMR S. aureus at the human-animal interface and understand the path of zoonotic transmission. This cross-sectional observational study aimed to assess the genetic diversity and AMR patterns of S. aureus isolated from cattle and humans on conventional and organic Vermont dairy farms that produce and sell farmstead cheese. Results A convenience sample of 19 dairy farms in Vermont was enrolled, and 160 S. aureus isolates were collected from cow quarter milk (CQM), bulk tank milk (BTM), human-hand and -nasal swabs. After deduplication, 89 isolates were used for the analysis. Sequence types (STs) were determined by multilocus sequence typing and cataloged to the PubMLST database. Nine defined and five novel STs were identified. For BTM and CQM samples, six STs were identified within cow-adapted CC97 and CC151. Two human-adapted STs were isolated from BTM and CQM. Seven human-adapted clonal complexes with eight STs were identified from human samples. One cow-adapted ST was isolated from a human. Antimicrobial susceptibility of the isolates was tested using disc diffusion and broth microdilution methods. Approximately 27% of the isolates were beta-lactam resistant and blaZ gene-positive. S. aureus isolates from human swabs were more likely to carry blaZ compared to isolates from CQM or BTM. S. aureus isolated from cows and humans on the same farm belonged to different STs. Conclusion Humans were more likely to carry beta-lactam-resistant S. aureus compared to cows, and on organic farms only human-adapted blaZ positive STs were isolated from BTM. Moreover, we identified potential spillover events of S. aureus sequence types between host species. The presence of penicillin-resistant-human-adapted S. aureus on both organic and conventional dairy farms highlights a “One Health” concern at the junction of public and animal health requiring further surveillance.

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“…Antibiotic resistance of pathogens is a significant and increasing problem in veterinary medicine [ 5 , 6 , 7 ]. Comprehensive efforts to control antibiotic resistance of bacterial isolates from animals have been made, given the importance of the problem for animal health itself, as well as the animal–human interactions in a One Health approach [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Clinical Aspects of Bacterial Distribution and Antibiotic Resistance in the Reproductive System of Equids

Tyrnenopoulou

Fthenakis

2023

Antibiotics

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Antibiotic administration is a standard therapeutic practice for the treatment of reproductive disorders of equids. This might lead to undesirable microbial imbalance and could favour the acquisition of antibiotic resistance. Therefore, it is imperative for clinicians to understand patterns of antibiotic resistance when considering and developing treatment regimes. Continued engagement of clinicians with novel alternative approaches to treat reproductive infections would be essential in order to address this rising threat within the One Health perspective. The objectives of the present review were to present the bacterial infections in the reproductive system of equids (horses, donkeys), to upraise the literature related to the issue of antibiotic resistance of bacteria causing these infections and to discuss the topic from a clinical perspective. Initially, the review summarised the various infections of the reproductive system of equids (genital system of females, genital system of males, mammary glands) and the causal bacteria, providing relevant information about horses and donkeys. Subsequently, the clinical therapeutics of these infections were presented, taking into account the significance of antibiotic resistance of bacteria as a limiting factor in treating the infections. Finally, approaches to circumvent antibiotic resistance in clinical settings were summarized. It was concluded that awareness regarding antibiotic resistance in equine reproductive medicine would increase, as we would recognise the multifaceted problem of resistance. Actions and initiatives within the One Health approach, minimizing the potential dissemination of resistant strains to humans and to the environment, with specific applications in medicine of equids should be appropriately instituted internationally.

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Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective

Cited by 8 publications

References 85 publications

MRSA in Humans, Pets and Livestock in Portugal: Where We Came from and Where We Are Going

MRSA in Humans, Pets and Livestock in Portugal: Where We Came from and Where We Are Going

An observational study demonstrates human-adapted Staphylococcus aureus strains have a higher frequency of antibiotic resistance compared to cattle-adapted strains isolated from dairy farms making farmstead cheese

Clinical Aspects of Bacterial Distribution and Antibiotic Resistance in the Reproductive System of Equids

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